Development and validation of a protocol for cell line identification by MALDI-TOF MS

Intact cell MALDI-TOF mass spectrometry, a promising proteomic profiling method in farm animal clinical and reproduction research

“…A known amount of cells (usually 2×10 3 to 1×10 6 cells) is deposited on the target of a conductive MALDI metallic plate and mixed with an adequate matrix solution. The matrix consists of crystallized molecules, the three most commonly used being 3,5-dimethoxy-4hydroxycinnamic acid (sinapinic acid) [24][25][26][27][29][30][31][32][33], α-cyano-4-hydroxycinnamic acid (CHCA, alphacyano or alpha-matrix) [34,35] and 2,5-dihydroxybenzoic acid (DHB) [36]. A solution of one of these molecules is made, often in a mixture of highly purified water and UV-absorbing weak organic acid such as acetonitrile (ACN), which is present in a concentration ranging 40-60%.…”

Exosomes: A Paradigm in Drug Development against Cancer and Infectious Diseases

“…The detection and profiling are often hindered by the requirements of high purity and large sample amount. To circumvent these issues, several new, sensitive, and selective methodologies based on surface plasmon resonance (SPR), fluorescence fluctuation spectroscopy (FFS), and so forth have been applied in several reports in Table 2 [14][15][16][17][18][19]. Among these, SPR is the most popular label-free, real-time sensing technique [99].…”

“…Among these, SPR is the most popular label-free, real-time sensing technique [99]. Vogel and coworkers have developed a label-free SPR based methodology for the detection of exosomes derived from breast cancer cell lines (MCF-7, BT-474, and MDA-MB-231) [15]. They reported immunosensor surface has the ability to identify various exosomes as well as exosomal biomarkers [98,100].…”