Development and validation of a real time PCR for the detection of myxoma virus based on the diploid gene M000.5L/R

A real time Taqman RT-PCR for the detection of rabbit hemorrhagic disease virus 2 (RHDV2)

“…Screening for classical RHDV strains was performed by conventional RT-PCR followed by sequencing analysis of the amplicons obtained with primers RC-9 and RC-10 [23], also using the One Step RT-PCR kit (Qiagen, Hilden, Germany). Myxoma virus was investigated by qPCR as described by Duarte et al [24] with the FastStart TaqMan Probe Master Kit (Roche, Roche Diagnostics GmbH, Manheim, Germany). Cq values above 40 were considered negative.…”

Rabbit haemorrhagic disease virus 2 (RHDV2) outbreak in Azores: Disclosure of common genetic markers and phylogenetic segregation within the European strains

“…Screening for RHDV was performed by sequencing analysis of the amplicons obtained with primers RC-9 and RC-10, published by (Tham et al, 1999). The presence of myxoma virus DNA was ruled out with the method published by (Duarte et al, 2014a).…”

Tracking the Origin of a Rabbit Haemorrhagic Virus 2 Outbreak in a Wild Rabbit Breeding Centre in Portugal; Epidemiological and Genetic Investigation

“…All liver samples (n=13) were positive to RHDV2 by RT-qPCR [22] and negative to myxoma virus by qPCR [24]. Sequencing analysis of vp60 386 bp long fragments ruled out the concomitant presence of classical RHDV strains.…”

“…Screening for classical RHDV strains was performed by conventional RT-PCR followed by sequencing analysis of the amplicons obtained with primers RC-9 and RC-10 [23], also using the One Step RT-PCR kit (Qiagen, Hilden, Germany). Myxoma virus was investigated by qPCR as described by Duarte et al [24] with the FastStart TaqMan Probe Master Kit (Roche, Roche Diagnostics GmbH, Manheim, Germany). Cq values above 40 were considered negative.…”