Development and Validation of a Sensitive UHPLC-MS/MS Method for the Measurement of Gardneramine in Rat Plasma and Tissues and Its Application to Pharmacokinetics and Tissue Distribution Study

Zhao, Nan; Tan, Hao-ran; Chen, Qili; Sun, Qi; Wang, Lin; Song, Yang; Olounfeh, Kamara Mohamed; Meng, Fan‐hao

doi:10.3390/molecules24213953

Cited by 5 publications

(2 citation statements)

References 19 publications

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“…Nowadays, UHPLC-MS/MS has become one of the most efficient methods for analyzing complex biological samples due to its high selectivity and sensitivity [ 16 , 17 , 18 ]. Therefore, a simple, sensitive, and reliable UHPLC-MS/MS method was developed and validated to measure itampolin A in rat plasma and tissues for the first time in this paper.…”

Section: Introductionmentioning

confidence: 99%

Pharmacokinetics and Tissue Distribution of Itampolin A following Intragastric and Intravenous Administration in Rats Using Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry

Sun,

Liang,

Zhang

et al. 2024

Molecules

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Itampolin A, a natural brominated tyrosine alkaloid isolated from the sponge Iotrochota purpurea, has been shown to have good inhibitory effects in lung cancer cells as a p38α inhibitor. A simple, sensitive, and reliable ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) method has been established, validated, and applied to the study of the pharmacokinetics and tissue distribution of itampolin A following intragastric and intravenous administration. Itampolin A and theophylline (internal standard, IS) were extracted by the simple protein precipitation technique using methanol as the precipitating solvent. Chromatographic separation was achieved by using the optimized mobile phase of a 0.1% formic acid aqueous solution and acetonitrile in the gradient elution mode. Itampolin A and IS were detected and quantified using positive electrospray ionization in the multiple reaction monitoring mode with transitions of m/z 863.9 → 569.1 for itampolin A and m/z 181.1 → 124.1 for IS, respectively. The assay exhibited a linear dynamic range of 1–1600 ng/mL for itampolin A in biological samples and the low limit of quantification was 1 ng/mL. Non-compartmental pharmacokinetic parameters indicated that itampolin A was well-absorbed into the systemic circulation and rapidly eliminated after administration. The apparent distribution volume of itampolin A was much higher after intragastric administration than that after intravenous administration. A tissue distribution study showed that itampolin A could be detected in different tissues and maintained a high concentration in the lung, which provided a material basis for its effective application in lung cancer. The pharmacokinetic process and tissue distribution characteristics of imtapolin A were expounded in this study, which can provide beneficial information for the further research and clinical application of itampolin A.

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Section: Introductionmentioning

confidence: 99%

Pharmacokinetics and Tissue Distribution of Itampolin A following Intragastric and Intravenous Administration in Rats Using Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry

Sun,

Liang,

Zhang

et al. 2024

Molecules

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“…A sensitive UHPLC-MS/MS method has been published by F.-H. Meng et al in their interesting article entitled “ Development and Validation of a Sensitive UHPLC-MS/MS Method for the Measurement of Gardneramine in Rat Plasma and Tissues and Its Application to Pharmacokinetics and Tissue Distribution Study ” [ 6 ]. Gardneramine is a monoterpenoid indole alkaloid with an exceptional central depressive effect and actions on myocardium.…”

mentioning

confidence: 99%