Development and validation of a high-performance liquid chromatography method using diode array detection for the simultaneous quantification of aripiprazole and dehydro-aripiprazole in human plasma

Development and validation of a rapid and sensitive LC-MS/MS method for the determination of aripiprazole in human plasma: Application to a bioequivalence study

“…Previous reports have used either liquid-liquid extraction (LLE) [13,15,18,20,21] or solid phase extraction (SPE) [14,19] for quantitative extraction of ARP from human plasma. However, protein precipitation was tried initially (due to high protein binding of the drug) using methanol, ethanol and acetonitrile as precipitating agents, but the response was insufficient with some ion suppression.…”

“…However, protein precipitation was tried initially (due to high protein binding of the drug) using methanol, ethanol and acetonitrile as precipitating agents, but the response was insufficient with some ion suppression. Thus, LLE was carried out in different solvent systems like dichloromethane, n-butyl chloride, diethyl ether, heptane, and MTBE, alone and in combination as described previously [13,15,18,20,21]. However, the response found was inconsistent especially at the LLOQ level in majority of the solvent systems (>20%), except MTBE.…”

“…Several analytical methods are reported to quantify ARP in different biological matrices, either alone [11][12][13][14][15][16], together with its active metabolite [17][18][19][20][21], or in combination with other antipsychotic and antidepressant drugs [22][23][24][25]. Therapeutic monitoring of ARP in serum samples by highperformance liquid chromatography (HPLC) with column-switching and spectrophotometric detection has been proposed by Kirschbaum and coworkers [11].…”

“…An improved LC-MS/MS method to determine serum levels of ARP and its metabolite in patients has been proposed by Caloro and co-workers [17]. All other methods [13][14][15][18][19][20][21] describe estimation of ARP in human plasma by gas chromatography-mass spectrometry (GC-MS) [19], capillary electrophoresis [14], HPLC [14,20], or LC-MS/MS [13,15,18,21] techniques. The salient features of these methods have been compiled in Table I.…”

“…The salient features of these methods have been compiled in Table I. Majority of these methods have a sensitivity which is ≥2 ng mL −1 [13-15, 19, 20] with very long chromatographic run time (5-17 min) [13][14][15][18][19][20][21]. Kubo et al [18] have developed a highly sensitive method for ARP and its active metabolite with a linear calibration range of 0.1-100 ng mL −1 .…”