Development and validation of a quantitative ELISA for the measurement of PSA concentration

Acevedo, Boris; Perera, Yasser; Ruiz, M. Pilar; Rojas, Gertrudis; Benítez, Jesús; Ayala, Marta; Gavilondo, Jorge V.

doi:10.1016/s0009-8981(01)00749-5

Cited by 93 publications

(46 citation statements)

References 28 publications

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“…Since its development in the early 1970's, the key word BELISA^has appeared in over 550,000 articles in the scientific database BWeb of Science^ (Fu et al 2006). ELISA is widely used in detecting and quantifying proteins such as prostate-specific antigen (Acevedo et al 2002), cytokines (Leng et al 2008), immunoglobulins (Engvall and Perlmann 1971;Griffin et al 2005) and breast cancer markers (Levesque et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Acoustic micromixing increases antibody-antigen binding in immunoassays

Gao

Tran

Petkovic-Duran

et al. 2015

Biomed Microdevices

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Sound wave-assisted acoustic micromixing has been shown to increase the binding of molecules in small volumes (10-100 μL) where effective mixing is difficult to achieve through conventional techniques. The aim of this work is to study whether acoustic micromixing can increase the binding efficiency of antibodies to their antigens, a reaction that forms the basis of immunoassays, including enzyme-linked immunosorbent assay (ELISA). Using a procedure from a general ELISA and immobilizing an antigen on wells of 96-well plates, it was found that acoustic micromixing at 125-150 Hz increased the initial rate of antibody-antigen binding by over 80 % and the total binding at the end point (i.e., 45 min) by over 50 %. As a result, acoustic micromixing achieved a binding level in 9 min that would otherwise take 45 min on a standard platform rocking mixer. Therefore acoustic micromixing has the potential to increase the detection sensitivity of ELISA as well as shorten the antigen-antibody binding times from typically 45-60 min to 15 min.

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Section: Introductionmentioning

confidence: 99%

Acoustic micromixing increases antibody-antigen binding in immunoassays

Gao

Tran

Petkovic-Duran

et al. 2015

Biomed Microdevices

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“…In recent years, most detection methods for PSA such as enzyme linked immunosorbent assay (ELISA) [7], radioimmunoassay [8], chemiluminuescent immunoassay [9] and immunosensors [10] are based on antibody due to their high selectivity to the antigen. However, the modification and the in-vivo preparation of antibodies are difficult, high-cost and time consuming.…”

Section: Introductionmentioning

confidence: 99%

Detection of the human prostate-specific antigen using an aptasensor with gold nanoparticles encapsulated by graphitized mesoporous carbon

Liu

Hua

et al. 2012

Microchim Acta

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We report on a sensitive electrochemical aptasensor for the detection of human prostate specific antigen (PSA). It is based on the signal amplification of the biotin-avidin system using a sensing platform that is making use of a graphite electrode modified with gold nanoparticles that were covered with graphitized mesoporous carbon nanoparticles (AuNPs@GMCs). The AuNPs@GMCs hybrid was prepared by linking 1,6-hexanedithiol-functionalized GMCs and gold nanoparticles via Au-S groups. Then, streptavidin was immobilized on the electrode modified with the AuNPs@GMCs so to enlarge the amount of biotin-aptamer which led to enhanced detection sensitivity. If an PSA aptamer captures the target PSA on the electrode, the differential pulse voltammetric (DPV) signal of the hexacyanoferrate redox system decreases. Factors affecting the performance of the aptasensor were studied in detail. Under optimal conditions, the DPV signal changes could be used to quantitatively detect PSA in the concentration range from 0.25 to 200 ng mL −1 , with a lowest limit of detection as small as 0.25 ng mL −1 . The aptasensor is highly specific and displays acceptable precision, good stability and repeatability.

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“…Unfortunately, most of the devices developed to diagnose various cancers such as prostate and lung cancer are too complicated and expensive to operate without scientific experience and information at outside of hospital and laboratory. For example, enzyme immunoassays (EIAs) with several detections (e.g., colorimeter, chemiluminescence, electrochemical, and fluorescence) capable of quantifying prostate specific antigen (PSA) in human serum were developed to early diagnose and prognose prostate cancer (Acevedo et al, 2002;Lee et al, 2010;Panini et al, 2008;Zheng et al, 2008). Unfortunately, sandwich EIAs using expensive capture antibody and detection antibody-conjugated enzyme (e.g., horseradish peroxidase and alkaline phosphatase) produced from the sacrifice of small animals are complicated and time-consuming even though the method is very sensitive (Acevedo et al, 2002;Lee et al, 2010;Panini et al, 2008;Zheng et al, 2008).…”

Section: Introductionmentioning

confidence: 99%