Development and validation of a screening procedure of microalgae for biodiesel production: Application to the genus of marine microalgae Nannochloropsis

Taleb, Ahmed; Pruvost, Jérémy; Legrand, Jack; Marec, H.; Le-Gouic, B.; Mirabella, Boris; Légeret, Bertrand; Bouvet, Séverine; Peltier, Gilles; Li‐Beisson, Yonghua; Taha, Samir; Takache, Hosni

doi:10.1016/j.biortech.2014.11.068

Cited by 59 publications

(52 citation statements)

References 29 publications

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“…It consisted of six small-scale photobioreactors (bubble columns) run in parallel, each tube having a volume V r = 3 · 10 −5 m 3 , an illuminated area of S L = 0.008 m 2 and a specific illuminated area of a light = S/V r of 266.7 1/m. A full description of the EOSS-PBR is done in Taleb et al [18].…”

Section: Culture Medium Screening In Efficient Overproducing Screeninmentioning

confidence: 99%

“…Before inoculation, the culture medium was filtered through a 0.45 μm pore size filter to remove undesirable small particles. The pH in the culture medium was around 7.5 for the freshwater microalgae species and 8 for the marine microalgae species, according to Pruvost et al and Taleb et al [17,18]. The culture agitation was provided by continuous injection of air with 2 vol.% CO 2 at a flow rate of 3 mL/min; the incident photon flux density (PFD), by a set of 6 fluorescent white tubes was~150 μmol/m 2 ·s.…”

Section: Culture Medium Screening In Efficient Overproducing Screeninmentioning

confidence: 99%

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Microalgae cultivation in urban wastewater: Nutrient removal and biomass production for biodiesel and methane

et al. 2015

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The freshwater microalgae species Chlorella kessleri and Chlorella vulgaris, and the marine microalgae species Nannochloropsis oculata were cultivated in urban wastewater. The freshwater species demonstrated the possibility of growing in urban wastewater reaching high biomass production and nutrient removal when cultured in batch mode using a flat-panel airlift photobioreactor. Both microalgae species reached high biomass dry weights, 2.70 ± 0.08 g/L and 2.91 ± 0.02 g/L respectively, accompanied by nitrogen concentration reduction around 96% and 95%, and a phosphorous concentration reduction around 99% and 98% respectively. N. oculata was able to uptake nutrients from wastewater to grow but with less efficiency, indicating the need of microalgae acclimation or process optimisation to achieve high nutrient removals. During C. kessleri and C. vulgaris cultivation, the nitrogen consumption led to a progressive N-starvation process which increased the microalgae potential for biofuels production; both species produced 346 ± 3 mL CH 4 /g VS and 415 ± 2 mL CH 4 /g VS during anaerobic digestion, and 7.4 ± 0.2 g Biodiesel /100 g VS and 11.3 ± 0.1 g Biodiesel /100 g VS respectively.

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Section: Culture Medium Screening In Efficient Overproducing Screeninmentioning

confidence: 99%

Section: Culture Medium Screening In Efficient Overproducing Screeninmentioning

confidence: 99%

Microalgae cultivation in urban wastewater: Nutrient removal and biomass production for biodiesel and methane

et al. 2015

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“…Some of the challenges include the wide biodiversity of microalgae and the dependency of their photosynthetic activity and especially their lipid metabolism on their cultivation conditions. Previously, Taleb et al, (2015) showed that TAG accumulation capacity cannot be the only criterion used to select the ideal strain. Many species are well known to accumulate large amount of neutral lipids and TAGs in nitrogen starvation conditions .…”

Section: Introductionmentioning

confidence: 99%

Screening of freshwater and seawater microalgae strains in fully controlled photobioreactors for biodiesel production

Taleb

Kandilian

Touchard

et al. 2016

Bioresource Technology

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Strain selection is one of the primary hurdles facing cost-effective microalgal biodiesel production. Indeed, the strain used affects both upstream and downstream biodiesel production processes. This study presents a screening procedure that considers the most significant criteria in microalgal biodiesel production including TAG production and wet extraction and recovery of TAGs. Fourteen freshwater and seawater strains were investigated. Large variation was observed between the strains in all the screening criteria. The overall screening procedure ultimately led to the identification of Parachlorella kessleri UTEX2229 and Nannochloropsis gaditana CCMP527 as the best freshwater and seawater strains, respectively. They featured the largest areal TAG productivity equal to 2.7×10(-3) and 2.3×10(-3)kgm(-2)d(-1), respectively. These two strains also displayed encouraging cell fragility in a high pressure bead milling process with 69% and 98% cell disruption at 1750bar making them remarkable strains for TAG extraction in wet environment.

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“…TAG) can be quantified [38–40] should be routinely adopted. The predictability of such high-throughput screenings should be validated at lab scale with the selected strains and processes should be developed under simulated outdoor production conditions [34]. Finally, the selected strains should be tested outdoors in pilot PBRs under relevant climate conditions [24, 41, 42].…”

Section: Discussionmentioning

confidence: 99%

Towards microalgal triglycerides in the commodity markets

Benvenuti

Ruiz²,

Lamers

et al. 2017

Biotechnol Biofuels

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BackgroundMicroalgal triglycerides (TAGs) hold great promise as sustainable feedstock for commodity industries. However, to determine research priorities and support business decisions, solid techno-economic studies are essential. Here, we present a techno-economic analysis of two-step TAG production (growth reactors are operated in continuous mode such that multiple batch-operated stress reactors are inoculated and harvested sequentially) for a 100-ha plant in southern Spain using vertically stacked tubular photobioreactors. The base case is established with outdoor pilot-scale data and based on current process technology.ResultsFor the base case, production costs of 6.7 € per kg of biomass containing 24% TAG (w/w) were found. Several scenarios with reduced production costs were then presented based on the latest biological and technological advances. For instance, much effort should focus on increasing the photosynthetic efficiency during the stress and growth phases, as this is the most influential parameter on production costs (30 and 14% cost reduction from base case). Next, biological and technological solutions should be implemented for a reduction in cooling requirements (10 and 4.5% cost reduction from base case when active cooling is avoided and cooling setpoint is increased, respectively). When implementing all the suggested improvements, production costs can be decreased to 3.3 € per kg of biomass containing 60% TAG (w/w) within the next 8 years.ConclusionsWith our techno-economic analysis, we indicated a roadmap for a substantial cost reduction. However, microalgal TAGs are not yet cost efficient when compared to their present market value. Cost-competiveness strictly relies on the valorization of the whole biomass components and on cheaper PBR designs (e.g. plastic film flat panels). In particular, further research should focus on the development and commercialization of PBRs where active cooling is avoided and stable operating temperatures are maintained by the water basin in which the reactor is placed.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-017-0873-2) contains supplementary material, which is available to authorized users.

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Development and validation of a screening procedure of microalgae for biodiesel production: Application to the genus of marine microalgae Nannochloropsis

Cited by 59 publications

References 29 publications

Microalgae cultivation in urban wastewater: Nutrient removal and biomass production for biodiesel and methane

Microalgae cultivation in urban wastewater: Nutrient removal and biomass production for biodiesel and methane

Screening of freshwater and seawater microalgae strains in fully controlled photobioreactors for biodiesel production

Towards microalgal triglycerides in the commodity markets

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