Development and validation of a novel multiplexed DNA analysis system, InnoTyper® 21

Brown, Hiromi; Thompson, Robyn; Murphy, Gina; Peters, Daniel R.; Rue, Bobby La; King, Jonathan L.; Montgomery, Anne; Carroll, Marion L.; Baus, James; Sinha, Sid; Wendt, Frank R.; Song, Bing; Chakraborty, Ranajit; Budowle, Bruce; Sinha, Sudhir K.

doi:10.1016/j.fsigen.2017.03.017

Cited by 24 publications

(10 citation statements)

References 43 publications

(75 reference statements)

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“…At 10 ng DNA input, no allelic artifacts were observed in the pig, rat, chicken, and E. coli DNA, while five allelic artifacts were observed in monkey DNA (Supporting Information Fig. 2) due to the genomic similarity between monkeys and humans [28]. No influential artifact generated from profiles of an increasing amount of E. coli DNA detected using the 18 Multi-InDels panel (Supporting Information Fig.…”

Section: Species Specificitymentioning

confidence: 98%

An 18 Multi‐InDels panel for analysis of highly degraded forensic biological samples

Lin

Jiang

et al. 2021

Electrophoresis

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DNA genotyping from trace and highly degraded biological samples is one of the most significant challenges of forensic DNA identification. There is a lack of simple and effective methods for genotyping highly degraded samples. In this study, a multiple loci insertion/deletion polymorphisms (Multi-InDels) panel was designed for detecting 18 autosomal Multi-InDels through capillary electrophoresis (CE) with amplicon sizes no longer than 125 bp. Studies of sensitivity, degradation, and species specificity were performed and a population study was carried out using 192 samples from Han populations in Hunan province in the south of China. The combined random match probability (CMP) of these 18 Multi-InDels was 3.23 × 10 -12 and the cumulative probability of exclusion (CPE) was 0.9989, suggesting this panel could be used independently for human identification and could provide efficient supporting information for parentage testing. Complete profiles were obtained from as low as 62.5 pg of total input DNA after increasing the number of PCR cycles. Moreover, all alleles were detected from artificially highly degraded DNA after 80 min of boiling water bath treatment. This 18 Multi-InDels panel is simple, fast, and effective for the forensic analysis of highly degraded DNA.

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Section: Species Specificitymentioning

confidence: 98%

An 18 Multi‐InDels panel for analysis of highly degraded forensic biological samples

Lin

Jiang

et al. 2021

Electrophoresis

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“…In this study, amplification was performed with InnoTyper ® 21 (Innogenomics), a bi-allelic marker assay targeting 20 retrotransposon insertion polymorphism loci and amelogenin [18].…”

Section: Introductionmentioning

confidence: 99%

“…In this study, amplification was performed with InnoTyper ® 21 (Innogenomics), a bi‐allelic marker assay targeting 20 retrotransposon insertion polymorphism loci and amelogenin [18]. The ForenSeq™ DNA Signature Prep chemistry primer set B (Verogen™, San Diego, USA) containing over 200 markers was also used.…”

Section: Introductionmentioning

confidence: 99%

Novel extraction chemistry and alternative amplification strategies for use with rootless hair shafts

Gutierrez

LaRue²,

Houston

2021

Journal of Forensic Sciences

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Rootless hair shafts are often considered unsuitable for STR genotyping due to the known high failure rate. The same samples can be reliably processed with mitochondrial sequencing. However, the minimal discriminatory power of widely implemented control region mitochondrial sequencing techniques limits its utility in some forensic casework. In this research, multiple variables were tested to provide information on rootless hair shaft sample genotyping success. Results showed external decontamination procedures decreased drop-in alleles but also greatly reduced profile recovery. The novel InnoXtract ™ chemistry was comparable to automated EZ1 DNA Investigator extraction. With thoroughly decontaminated hairs, InnoTyper ® 21 amplification generated random match probabilities higher than STR chemistry in 71.875% of samples and 18.75% of samples benefitted from the use of InnoTyper ® 21 amplification compared with estimated mtDNA profile rarity. Compared with the capillary electrophoresis-based amplification chemistries tested, the ForenSeq™ DNA Signature Prep chemistry paired with massively parallel sequencing was the most discriminatory amplification strategy tested.

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“…The kit improved discrimination power between Y-STR profiles of related and unrelated males compared to conventional Y-STRs (17,18). Despite the features improvement of Y-SYR, there is a need to develop new methods to allow the complete DNA profile of the unbalanced mixed stains to be determined regardless of the gender of the contributors; two methods have been developed for this purpose: DIP-STR and SNP-STR (18)(19)(20)(21)(22). These markers, marked on autosomal and sex chromosomes, show the expected characteristics of forensic medicine, such as the absence of a peak stutter, lower mutation rates than STRs, and short amplicons.…”

Section: Y Strsmentioning

confidence: 99%

“…These characteristics are of great importance for the analysis of destroyed samples, determination of human identity, human paternity, etc. (20)(21)(22)(23)(24)(25)(26)(27).…”

Section: Y Strsmentioning

confidence: 99%

Application of Y-STR, DIP-STR and SNP-STR Markers in Interpretation of Forensic Genetic Profiling: A Narrative Review

Forouzesh¹,

Irani²,

Soleimani³

et al. 2022

ijph

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Y-STR, DIP-STR, and SNP-STR are useful alternatives for testing the low quantity of DNA in solving the challenges in interpreting forensic genetic profiling. In an unbalanced mixed DNA, partial DNA is often not detected due to the effect of masking by the dominant DNA. Therefore, in such cases interpretation of the results is limited. Furthermore, profiling of these specimens cannot be performed using conventional forensic genetic methods. Biomarkers including Y-STR, DIP-STR and SNP-STR perform well in detecting DNA contributes in the mixed sample. In the present research, the performance of each is evaluated separately.

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Development and validation of a novel multiplexed DNA analysis system, InnoTyper® 21

Cited by 24 publications

References 43 publications

An 18 Multi‐InDels panel for analysis of highly degraded forensic biological samples

An 18 Multi‐InDels panel for analysis of highly degraded forensic biological samples

Novel extraction chemistry and alternative amplification strategies for use with rootless hair shafts

Application of Y-STR, DIP-STR and SNP-STR Markers in Interpretation of Forensic Genetic Profiling: A Narrative Review

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