Development and validation of an ultra-high performance liquid chromatography–tandem mass-spectrometry (UHPLC–MS/MS) method for the simultaneous determination of neurotransmitters in rat brain samples

González, Roberto Romero; Fernández, Remedios Fernández; Vidal, José Luis Martı́nez; Frenich, Antonia Garrido; Pérez, María Luz Gómez

doi:10.1016/j.jneumeth.2011.03.023

Cited by 148 publications

(71 citation statements)

References 35 publications

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“…Serotonin has been determined by several techniques, which integrate separation methods-such as liquid chromatography [36][37][38] and capillary electrophoresis 39 -or direct assays using potentiometry 27,29,40 and sensor-based applications. 41,42 In general, as verified in Table 1, our set up shows a LOD lower than those reported in the literature even when a preconcentration step was employed.…”

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confidence: 99%

Glass/PDMS hybrid microfluidic device integrating vertically aligned SWCNTs to ultrasensitive electrochemical determinations

et al. 2012

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This communication reports a promising platform for rapid, simple, direct, and ultrasensitive determination of serotonin. The method is related to integration of vertically aligned singlewalled carbon nanotubes (SWCNTs) in electrochemical microfluidic devices. The required microfabrication protocol is simple and fast. In addition, the nanomaterial influenced remarkably the obtained limit-of-detection (LOD) values. Our system achieved a LOD of 0.2 nmol L 21 for serotonin, to the best of our knowledge one of the lowest values reported in the literature.Glass/elastomer hybrid microfluidic platforms with integrated electrochemical detectors are powerful analytical tools. Their main advantages include: (i) low cost; (ii) chemical inertia; (iii) optical transparency; (iv) thermal, mechanical, and chemical stability; (v) automation capacity, and; (vi) easy microfabrication. In addition, they offer high values of reproducibility and sensitivity.

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confidence: 99%

Glass/PDMS hybrid microfluidic device integrating vertically aligned SWCNTs to ultrasensitive electrochemical determinations

et al. 2012

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“…Using HPLC-MS/MS provides specific structural information with high sensitivity, fast separation speed, and is simple and quick. Therefore, this method has been increasingly used for different cerebral neurotransmitter quantitative analysis (Carrozzo et al, 2010;González et al, 2011). A recent study also used HPLC-MS/MS to simultaneously determine a number of rat brain neurotransmitters (Zhu et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective effects of alkaloids fromPiper longumin a MPTP-induced mouse model of Parkinson’s disease

Wang

et al. 2015

Pharmaceutical Biology

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“…For example, GABA is known to be relatively concentrated in brain tissue (ca. 50 µg/g in striatum and amygdala) [29] . Direct analysis (m/z 104→87), showed no localization to the white or gray matter of the cerebellum and significant background signal was obtained on target (not shown).…”

Section: Imaging Ms/ms Analysis Of Underivatized Amino Acids and Neurmentioning

confidence: 99%

A derivatization and validation strategy for determining the spatial localization of endogenous amine metabolites in tissues using MALDI imaging mass spectrometry

Manier¹,

Spraggins²,

Reyzer³

et al. 2014

J. Mass Spectrom.

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Imaging mass spectrometry (IMS) studies increasingly focus on endogenous small molecular weight metabolites and consequently bring special analytical challenges. Since analytical tissue blanks do not exist for endogenous metabolites, careful consideration must be given to confirm molecular identity. Here we present approaches for the improvement in detection of endogenous amine metabolites such as amino acids and neurotransmitters in tissues through chemical derivatization and matrix-assisted laser desorption/ionization (MALDI) IMS. Chemical derivatization with 4-hydroxy-3-methoxycinnamaldehyde (CA) was used to improve sensitivity and specificity. CA was applied to the tissue via MALDI sample targets precoated with a mixture of derivatization reagent and ferulic acid (FA) as a MALDI matrix. Spatial distributions of chemically derivatized endogenous metabolites in tissue were determined by high-mass resolution and MS n imaging mass spectrometry. We highlight an analytical strategy for metabolite validation whereby tissue extracts are analyzed by high-performance liquid chromatography (HPLC)-MS/MS to unambiguously identify metabolites and distinguish them from isobaric compounds.

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Development and validation of an ultra-high performance liquid chromatography–tandem mass-spectrometry (UHPLC–MS/MS) method for the simultaneous determination of neurotransmitters in rat brain samples

Cited by 148 publications

References 35 publications

Glass/PDMS hybrid microfluidic device integrating vertically aligned SWCNTs to ultrasensitive electrochemical determinations

Glass/PDMS hybrid microfluidic device integrating vertically aligned SWCNTs to ultrasensitive electrochemical determinations

Neuroprotective effects of alkaloids fromPiper longumin a MPTP-induced mouse model of Parkinson’s disease

A derivatization and validation strategy for determining the spatial localization of endogenous amine metabolites in tissues using MALDI imaging mass spectrometry

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