Development and validation of an RP‐HPLC method for quantitative determination of vanillin and related phenolic compounds in Vanilla planifolia

Abstract

A simple and fast method was developed using RP-HPLC for separation and quantitative determination of vanillin and related phenolic compounds in ethanolic extract of pods of Vanilla planifolia. Ten phenolic compounds, namely 4-hydroxybenzyl alcohol, vanillyl alcohol, 3,4-dihydroxybenzaldehyde, 4-hydroxybenzoic acid, vanillic acid, 4-hydroxybenzaldehyde, vanillin, p-coumaric acid, ferulic acid, and piperonal were quantitatively determined using ACN, methanol, and 0.2% acetic acid in water as a mobile phase with a gradient elution mode. The method showed good linearity, high precision, and good recovery of compounds of interest. The present method would be useful for analytical research and for routine analysis of vanilla extracts for their quality control.

Simultaneous Analysis of Guaiacol and Vanillin in a Vanilla Extract by Using High-Performance Liquid Chromatography with Electrochemical Detection

“…The respective LOD values for guaiacol and vanillin were 0.480 mg/L and 0.870 mg/L, and the respective LOQ values for guaiacol and vanillin were 1.60 mg/L and 2.36 mg/L. LOD and LOQ for vanillin by HPLC-ECD were approximately 30-1000 times lower than the values obtained by HPLC-UV in previous published studies, [15][16][17] and those for guaiacol were also similar to those for vanillin. HPLC-ECD detected both vanillin and guaiacol with good specificity and high sensitivity, and quantification at relatively low concentrations in the range 1-1000 mg/L enabled the target compounds to be selectively quantified without any influence from soluble solid in the vanilla extract.…”

“…[14][15][16][17] Four major components in vanilla, namely, p-hydroxybenzaldehyde (p-HBAld), p-hydroxybenzoic acid (p-HB acid), vanillic acid, and vanillin, can be easily measured by HPLC. 14) For this reason, the Direction Générale de la Concurrence, de la Consommation et de la Répression des Fraudes (DGCCRF) y To whom correspondence should be addressed.…”

Methoxybenzaldehydes in Plants:Insight to the Natural Resources, Isolation, Application and Biosynthesis

“…Though numerous quality methods are available for separation and quantification of compounds from vanilla extracts, including TLC, HPTLC (high performance thin layer chromatography), isotope ratio MS and HPLC, the search continues for a simple and rapid HPLC-based method for isolation of this fragrant methoxybenzaldehyde from ethanolic vanilla pod extract [23][24][25][26][27][28] . A RP-HPLC-based method was developed for efficient separation of compounds using which maximum number of compounds, including vanillin has been efficiently separated in a single run using RP Purospher ®-Star RP-18e column and a photodiode array detector 29 . In this method, ACN/methanol (solvent A, 1 : 1 v/v) and water/acetic acid (solvent B, 99.8 : 0.2 v/v, pH 2.88) were used as mobile phase with a gradient elution 29 .…”

“…A RP-HPLC-based method was developed for efficient separation of compounds using which maximum number of compounds, including vanillin has been efficiently separated in a single run using RP Purospher ®-Star RP-18e column and a photodiode array detector 29 . In this method, ACN/methanol (solvent A, 1 : 1 v/v) and water/acetic acid (solvent B, 99.8 : 0.2 v/v, pH 2.88) were used as mobile phase with a gradient elution 29 . Further, an ultra-high performance liquid chromatography (UHPLC) method was developed for rapid and better separation of vanillin from vanilla bean extract using a C 18 RP column (ACQUITY UPLC BEH C 18 , 50 mm  2.1 mm, 1.7 m particle size) 30 .…”

gIsolation and purification of acetylshikonin and β‐acetoxyisovalerylshikonin from cell suspension cultures of Arnebia euchroma (Royle) Johnston using rapid preparative HPLC*

“…The calibration graphs were plotted after linear regression of the peak areas versus concentrations. Limit of detection (LOD) and limit of quantitation (LOQ) were determined as in our earlier reports [44,45].…”