Development and validation of an ultra high performance liquid chromatography tandem mass spectrometry method for determination of 10 cephalosporins and desacetylcefapirin in milk

Recent bioanalytical methods for quantification of third‐generation cephalosporins using HPLC and LC‐MS(/MS) and their applications in pharmacokinetic studies

“…Quantitative determination of cefoperazone in human plasma (Tsujikawa et al ., ; Zhou et al ., ) and milk (Hou et al ., ; Junza et al ., ; Li et al ., ) was investigated using LC‐MS(/MS). For human plasma samples, protein precipitation (Tsujikawa et al ., ) and liquid–liquid extraction (Zhou et al ., ) were used for sample pre‐treatment, and cefoperazone was separated on a C 18 column (Tsujikawa et al ., ; Zhou et al ., ).…”

“…Calibration curves for cefoperazone were linear over the range 0.07–1.93 µg/mL in HPLC‐MS by SIM in positive mode ( m / z 646; Tsujikawa et al ., ), and 0.1–20 µg/mL in HPLC‐MS/MS by SRM in negative mode ( m / z 644.0 → m / z 115.0; Zhou et al ., ). For bovine milk samples, after liquid–liquid extraction (Li et al ., ), SPE (Hou et al ., ; Junza et al ., ; Li et al ., ) and SPE with C 18 ‐Fe 3 O 4 @mSiO 2 microspheres (Liu et al ., ) for sample processing, cefoperazone was separated from endogenous peaks using C 8 (Junza et al ., ) and C 18 (Hou et al ., ; Junza et al ., ; Li et al ., ; Liu et al ., ) columns. HPLC coupled to tandem mass spectrometry detection (MS/MS) was applied for cefoperazone analysis by multiple reaction monitoring mode (MRM) in positive mode, and calibration curves for cefoperazone were linear over the range of 0.5–1.25 µg/kg ( m / z 646 → m / z 290; Junza et al ., ) and 2–5000 ng/mL ( m / z 530; Liu et al ., ).…”

Detection of the iron complexes with hydrolysis products of cephalexin and cefradine upon high‐performance liquid chromatography/electrospray ionization mass spectrometry analysis

“…Electrospray ionization mass spectrometry, usually coupled with high‐performance liquid chromatography (HPLC/ESI‐MS), has been successfully applied to their analysis . However, there is little nuclear magnetic resonance (NMR) data reported concerning the products of their hydrolysis and, to the best of our knowledge, there is no ESI‐MS data reported concerning the products.…”

New valid spectrofluorimetric method for determination of selected cephalosporins in different pharmaceutical formulations using safranin as fluorophore

“…The wide use of these antibiotics in modern antimicrobial therapy is due to their enhanced intrinsic microbiological activities and favorable safety profile. Chemically cephalosporin antibiotics are derived from the 7-aminocephalosporanic acid (7-ACA) which composed of a β-lactam ring fused with a dihydrothaizine ring (Table 1) Several methods have been reported for the quantitative determination of the cited cephalosporins including spectrophotometry [2][3][4][5][6][7][8], spectrofluorimetry [8][9][10][11][12][13][14], chemiluminescence [15], High performance liquid chromatography (HPLC) [16][17][18], Thin layer chromatography [19], capillary electrophoresis (CE) [20,21] and voltammetry [22][23][24][25][26].…”