Development and validation of an improved method for the analysis of vancomycin by liquid chromatography

Diana, J; Visky, D.; Roets, Eugène; Hoogmartens, Jos

doi:10.1016/s0021-9673(03)00535-1

Cited by 29 publications

(19 citation statements)

References 46 publications

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“…For ceftriaxone the chromogen was linear over the range of 2.0-36 µg/ml. The separation and identification of vancomycin was done by thin-layer chromatography, normal and reverse phases chromatography [21][22][23][24] . Thus we developed a single method for the combined analysis of both drugs, saving time and other resources.…”

Section: Methods Development and Validationmentioning

confidence: 99%

“…In the clinical laboratory the use of this technique has been efficient for determination of analytes in several biological fluids. Therefore, HPLC has been developed for the analysis of vancomycin in medicines, as well as for the determination in biological fluids 17,24,[26][27][28][29][30][31][32][33][34] . Kitahashi and Furuta 35 developed an assay for measuring vancomycin concentration by micellar electrokinetic capillary chromatography with detection at 210 nm using direct serum injection.…”

Section: Clinical Applicationmentioning

confidence: 99%

See 1 more Smart Citation

Untitled

Tariq¹,

Siddiqui²,

Kumar³

et al. 2010

ScienceAsia

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A reverse phase-liquid chromatographic method with UV detection at 280 nm is described for simultaneous determination of ceftriaxone sodium and vancomycin hydrochloride. Chromatographic separation of the two drugs was achieved on a Betasil C-1 column using a mobile phase consisting of a binary mixture of acetonitrile and triethylamine buffer adjusted to pH 3.5 ± 0.1 with orthophosphoric acid in a ratio of 20:80. The liquid chromatographic method developed offers symmetric peak shape, good resolution, and reasonable retention time for both drugs. Linearity, accuracy, and precision were found to be acceptable over the concentration ranges 125-750 ppm for ceftriaxone and 62.5-375 ppm for vancomycin. The liquid chromatographic method was successfully applied to the quality control of formulated products, plasma, and cerebrospinal fluid samples containing ceftriaxone and vancomycin.

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Section: Methods Development and Validationmentioning

confidence: 99%

Section: Clinical Applicationmentioning

confidence: 99%

Untitled

Tariq¹,

Siddiqui²,

Kumar³

et al. 2010

ScienceAsia

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show abstract

“…In the clinical laboratory the use of this technique has been efficient for analytes determination of in several biologic fluids. Therefore, HPLC technique has been developed for the analysis of vancomycin in medicines [48][49][50][51][52] , as well as for the determination in biological fluids 25,26,53-67 . Generally, it was observed that the majority of the applied methods in the determination of vancomycin with HPLC use UV detectors, working in a wavelength range from 198 nm 59 to 282 nm 60 .…”

Section: Chromatographic Methodsmentioning

confidence: 99%

Analytical methods for vancomycin determination in biological fluids and in pharmaceuticals

et al. 2007

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Recebido em 25/8/05; aceito em 7/4/06; publicado na web em 31/10/06Vancomycin is a glycopeptide antibiotic employed in the treatment of infections caused by certain methicillin-resistant staphylococci. It is indicated also for patients allergic to penicillin or when there is no response to penicillins or cephalosporins. The adequate vancomycin concentration levels in blood serum lies between 5 and 10 mg/L. Higher values are toxic, causing mainly nephrotoxicity and ototoxicity. Various analytical methods are described in the literature: spectrophotometric, immunologic, biologic and chromatographic methods. This paper reviews the main analytical methods for vancomycin determination in biological fluids and in pharmaceutical preparations.

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“…The concentrations were calculated from the corresponding calibration standard line (experimental concentrations) and were compared with the theoretical concentrations [32][33][34][35][36][37][38]. The extraction recovery was estimated according to Equation 1 and is shown in Table 2.…”

Section: Linearity and Loqmentioning

confidence: 99%