Development and validation of an LC–MS/MS method for the quantification of artificial sweeteners in human matrices

Greibe, Eva; Leth-Møller, Magnus; Stampe, Sofie; Ovesen, Per; Pedersen, Michael; Hoffmann-Lücke, Elke

doi:10.1002/bmc.5350

Cited by 7 publications

(5 citation statements)

References 10 publications

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“…AUC ratios may look different if calculated after complete elimination, but this was not possible in our study. Unfortunately, it was not possible to calculate the AUC ratios for sucralose as a majority of the samples fell below the LOQ of the analytical method [22].…”

Section: Discussionmentioning

confidence: 99%

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Artificial Sweeteners in Breast Milk: A Clinical Investigation with a Kinetic Perspective

et al. 2022

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Artificial sweeteners (ASs) are calorie-free chemical substances used instead of sugar to sweeten foods and drinks. Pregnant women with obesity or diabetes are often recommended to substitute sugary products with ASs to prevent an increase in body weight. However, some recent controversy surrounding ASs relates to concerns about the risk of obesity caused by a variety of metabolic changes, both in the mother and the offspring. This study addressed these concerns and investigated the biodistribution of ASs in plasma and breast milk of lactating women to clarify whether ASs can transfer from mother to offspring through breast milk. We recruited 49 lactating women who were provided with a beverage containing four different ASs (acesulfame-potassium, saccharin, cyclamate, and sucralose). Blood and breast milk samples were collected before and up to six hours after consumption. The women were categorized: BMI < 25 (n = 20), BMI > 27 (n = 21) and type 1 diabetes (n = 8). We found that all four ASs were present in maternal plasma and breast milk. The time-to-peak was 30–120 min in plasma and 240–300 min in breast milk. Area under the curve (AUC) ratios in breast milk were 88.9% for acesulfame-potassium, 38.9% for saccharin, and 1.9% for cyclamate. We observed no differences in ASs distributions between the groups.

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Section: Discussionmentioning

confidence: 99%

“…Analytical separation was performed on a Luna Omega C18 column (2.1 × 50 mm, 1.6 µm) (Phenomenex, Copenhagen, Denmark) at a temperature of 30 • C controlled by a column heater. The method is described in detail by Greibe et al [22].…”

Section: Biochemical Analysesmentioning

confidence: 99%

Artificial Sweeteners in Breast Milk: A Clinical Investigation with a Kinetic Perspective

et al. 2022

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“…The RP-HPLC purified sample was analyzed with LCMS/MS (16)(17) and the synthesized antimicrobial peptide (AMP) sample exhibited a wide range of antimicrobial activity against Staphylococcus aureus-ATCC 25923/ MCC 2408, Pseudomonas aeruginosa-ATCC 27853/ MCC 2080, Aspergillus niger-MTCC 9983 and Candida albicans-MTCC 183 which represent Gram-positive, Gram-negative, and fungal pathogens. High-performance liquid chromatography authorizes us to separate the peptide mixture, and protein sequence was identified with potential antimicrobial peptide from MS/MS data through de novo sequencing.…”

Section: Discussionmentioning

confidence: 99%

Characterization and biochemical activities of novel functional antimicrobial peptide (AMP) from Trichogramma chilonis

Sunil¹,

Kumar²,

Pramod³

et al. 2022

Biomedicine

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Introduction and Aim: The antimicrobial peptides (AMPs) are generally found in invertebrates, mammals, birds, plants and insects. AMPs produced by insect parasitoids contribute to innate immunity to resist infection due to lack of adaptive immunity. T. chilonis is one of the most effective endoparasitoid wasps for controlling lepidopterous insects. Several attempts have been made to isolate, characterize and develop a commercially viable product of AMPs from various insect sources. The present study aimed to characterize AMP from T. chilonis for potential antimicrobial and anti-cancer properties. Methods: AMP was identified through T. chilonis transcriptome sequence and designed in silico and synthesized. Its purity was quantified using RP-HPLC, and the mass identified by mass spectrophotometry. LC/MS-MS was employed to predict the sequence and the BLAST program used to compare the sequence. AMP was tested for haemolytic activity and antimicrobial activity. Two pathogenic bacteria and fungal strains were used and IC50 values and MIC values were predicted against microbial strains. Results: Synthetic peptide was found to be 95% homogenous with molecular weight of 3.48 kD. The peptide was identified to be a novel antimicrobial peptide consisting of 33 amino acid residues, and has a low computed instability index of -0.1.55 with high hydrophobic ratio of 27.27%. The antimicrobial activity revealed that T. chilonis antimicrobial peptide (TC-AMP) strongly inhibits the growth of selected human bacterial and fungal pathogens. While the haemolytic assay showed that the peptide did not obliterate human RBC in vitro. TC-AMP also showed an efficient inhibition of angiogenesis by in vivo model as evident by inhibition of vascularization. Conclusions: AMP derived from the parasitoid has a potent antibiotic and anti-angiogenesis property. The peptide can be used as a potential antimicrobial and anticancer drug in near future with more detailed studies on its targeted applications.

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“…The method for measuring the sweeteners is described elsewhere [12]. In brief, concentrations of the ASs were measured by High-Performance Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS).…”

Section: Methodsmentioning

confidence: 99%

“…Forty-five women were invited to participate, of whom thirty-five accepted. In the control group, one participant was excluded because of an intake of an AS on the morning The method for measuring the sweeteners is described elsewhere [12]. In brief, concentrations of the ASs were measured by High-Performance Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS).…”

Section: Participantsmentioning

confidence: 99%

Transplacental Transport of Artificial Sweeteners

et al. 2023

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The prevalence of obesity is increasing, and the origins of obesity and metabolic dysfunction may be traced back to fetal life. Currently, overweight pregnant women are advised to substitute sugar-sweetened beverages with diet drinks containing artificial sweeteners. Recent evidence suggests that the consumption of artificial sweeteners during pregnancy increases the risk of obesity in the child, but the mechanism is unknown. We hypothesized the transportation of artificial sweeteners across the placenta into the fetal circulation and the amniotic fluid. We included 19 pregnant women who were given an oral dose of acesulfame, cyclamate, saccharin, and sucralose immediately before a planned caesarean section. Nine women were included as controls, and they refrained from an intake of artificial sweeteners. The maternal and fetal blood and amniotic fluid were collected during the caesarean section, and concentrations of artificial sweeteners were measured using mass spectrometry. We found a linear relationship between the fetal plasma concentrations of artificial sweeteners and the maternal plasma concentrations, with adjusted coefficients of 0.49 (95% CI: 0.28–0.70) for acesulfame, 0.72 (95% CI: 0.48–0.95) for cyclamate, 0.51 (95% CI: 0.38–0.67) for saccharin, and 0.44 (95% CI: 0.33–0.55) for sucralose. We found no linear relationship between amniotic fluid and fetal plasma concentrations, but there were positive ratios for all four sweeteners. In conclusion, the four sweeteners investigated all crossed the placenta and were present in the fetal circulation and amniotic fluid.

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Development and validation of an LC–MS/MS method for the quantification of artificial sweeteners in human matrices

Cited by 7 publications

References 10 publications

Artificial Sweeteners in Breast Milk: A Clinical Investigation with a Kinetic Perspective

Artificial Sweeteners in Breast Milk: A Clinical Investigation with a Kinetic Perspective

Characterization and biochemical activities of novel functional antimicrobial peptide (AMP) from Trichogramma chilonis

Transplacental Transport of Artificial Sweeteners

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