Development and validation of an HPLC-DAD analytical method to quantify 5-methoxyflavones in methanolic extracts of Vochysia divergens Pohl cultured under stress conditions

Abstract

Vochysia divergens Pohl, known as "Cambara" in Brazil, is an invasive species that is expanding throughout Pantanal in Brazil, to form mono-dominant communities. This expansion is affecting the agricultural areas that support the typical seasonal flood and drought conditions of this biome. This article describes the development and validation of an HPLC-DAD analytical method to quantify 5-methoxyflavones in methanolic extracts of greenhouse-grown V. divergens associated with one of two endophytic fungal species Zopfiella tetraspora (Zt) or Melanconiella elegans (Me) and later subjected to water stress. The developed method gave good validation parameters and was successfully applied to quantify the flavones 3′,5-dimethoxy luteolin-7-O-β-glucopyranoside (1), 5-methoxy luteolin (2), and 3′,5-dimethoxy luteolin (3) in the target extracts. Inoculation of the plant with Zt decreased the concentration of flavone 1 in the extract by 2.69-fold as compared to the control. Inoculation of the plant with Zt or Me did not significantly alter the contents of flavones 2 and 3 in the extracts as compared to the control. Therefore, the aerial parts of germinated V. divergens plants inoculated with either Zt or Me responded differently in terms of the production of flavones. These results can cast light on the symbiosis between fungal microorganisms and V. divergens, which most likely influences the response of V. divergens to changes in the availability of water in Pantanal.

Genotoxic and Chemopreventive Effects of Vochysia divergens Leaves (Pantanal, Brazil)

“…The VdE presents flavones in its chemical composition, being the flavone 3′5 DL its major component [9, 10]. Polyphenolic compounds, as flavones, can act as prooxidants in some in vitro systems [16].…”

“…Pimenta et al [9, 10] demonstrated flavones as constituents of Vochysia extracts. Flavones are a class of flavonoids that are a subject of increasing interest because of their biological activities in vitro and in vivo , especially their antioxidant activity.…”

“…Reverse-phase ODS chromatography was performed with Fraction 3 (80 mg), which was purified by preparative RPHPLC [CH3OH-H2O-CH3COOH (50:49.9:0.1, v/v/v)] to yield 3′5 DL (10 mg) [9]. The flavone was quantified in VdE by HPLC-DAD according to Pimenta et al [10]. …”

Metabolomic Analysis of Combretum lanceolatum Plants Interaction with Diaporthe phaseolorum and Trichoderma spirale Endophytic Fungi through 1H‐NMR

“…Plants are the basis of our daily diet, providing primary‐metabolite nutrients such as carbohydrates, amino acids and lipids as well as a wide variety of secondary metabolites such as flavonoids, alkaloids, coumarins, terpenoids and steroids. Plant based metabolites have found widespread application in the agrochemical and pharmaceutical industries as well as food additives and flavours [1] . Secondary metabolites perform different roles in plant physiological processes and mediate their interactions with physical, chemical and biological agents, mainly by regulating the biochemical and molecular changes which the plant undergoes as a response to these stimuli [2] …”

Chemical composition and in vitro cytotoxicity of Corynespora olivacea (V18) associated with Vochysia divergens / Composição química e citotoxicidade in vitro de Corynespora olivacea (V18) associada a Vochysia divergens

“…Because the association of V. divergens with endophytic microorganisms is poorly understood, and since the chemical profile and biological potential of its microbiota remains partially unknown, our research group has been dedicated to the chemical study of this plant species (Pimenta et al, 2015) and its chemical interaction with its endophytic microbiota (Parpinelli et al, 2017;Pimenta et al, 2017;de Oliveira et al, 2018;Farias et al, 2019).…”