Development and validation of analytical method for simultaneous estimation of amlodipine besylate and celecoxib in pure and combined dosage form

Mandale, Tejaswini Rajaram; Manish, Kondawar; Kadam, Sandeep Dilip

doi:10.5958/0974-360x.2020.00756.8

Cited by 5 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this study, the proposed methods for the determination of Metformin Hydrochloride and Pravastatin Sodium were carried out by two analysts [14].…”

Section: Ruggednessmentioning

confidence: 99%

Development and Validation of UV-Spectroscopic Method for Simultaneous Estimation of Metformin Hydrochloride and Pravastatin Sodium

SHARMA

Verma

Kumar

et al. 2023

J. Pharm. Res. Int.

View full text Add to dashboard Cite

Introduction: A new, simple, precise, accurate, and reproducible method was developed and validated for the simultaneous estimation of Metformin Hydrochloride (MH) and Pravastatin Sodium (PS) in pure form. Methodology: Simultaneous estimation of Metformin Hydrochloride (MH) and Pravastatin Sodium (PS) was estimated by ultraviolet (UV) spectrophotometry using the absorbance subtraction method. The method was based on the measurement of absorbance at two wavelengths 232 nm and 238 nm, of metformin and Pravastatin Sodium respectively. These studies were performed at three different levels (75%, 100%, and 125%) and the % recovery of MH and PS was calculated. Results: The LOD and LOQ were found to be 0.481 μg/ml and 0.670μg/ml for MH and 1.15μg/ml and 1.68μg/ml for PS respectively. All the statically analyses were within the standard limits. It proves that the method was repeatable and selective for the simultaneous. Conclusion: Therefore, the present study concludes that it can be successfully used for simultaneous estimation of MH and PS in pure and pharmaceutical dosage forms. The developed method was found to be simple, precise, and accurate.

show abstract

“…In this study, the proposed methods for the determination of Metformin Hydrochloride and Pravastatin Sodium were carried out by two analysts [14].…”

Section: Ruggednessmentioning

confidence: 99%

Development and Validation of UV-Spectroscopic Method for Simultaneous Estimation of Metformin Hydrochloride and Pravastatin Sodium

SHARMA

Verma

Kumar

et al. 2023

J. Pharm. Res. Int.

View full text Add to dashboard Cite

show abstract

Development and Validation of RP-HPLC Method for Estimation of Amlodipine Besylate and Celecoxib in Pharmaceutical Formulation

Nagamani¹,

Manjunath²,

Kumar³

2020

J. Drug Delivery Ther.

View full text Add to dashboard Cite

A simple, precise, accurate, and rapid reverse phase-high performance liquid chromatography (RP-HPLC) method with UV-Visible detector has been developed and subsequently validated for the simultaneous determination of amlodipine besylate(AML) and celecoxib(CEL) in their combined tablet dosage form. The separation was based on the use of a Flowrosil C18 analytical column (250 × 4.6 mm, i.d., 5 µm). The mobile phase consisted of a mixture of 80 volumes of acetonitrile and 20 volumes of water. The chromatography was performed by isocratic elution at a flow rate of 1 mL/min. Analytes were detected at 250 nm, with linear calibration curves at concentration ranges of 2-12 µg/ml and 50-300 µg/ml for AML and CEL respectively. The retention time of AML and CEL were 1.98 and 3.15 min respectively. The recoveries obtained were 99.46‒101.36% for AML, 99.57‒101.42% and 99.96–100.87 % for CEL. The method was validated according to International conference of harmonisation guidelines in terms of accuracy, precision, specificity, robustness, limits of detection and quantitation, and other aspects of analytical validation. The developed method was applied successfully for HPLC analysis of commercial pharmaceutical products including AML and CEL. Keywords: Amlodipine besylate; Celecoxib; RP-HPLC.

show abstract

Validated Green Chromatographic Methods for Determination of Amlodipine and Celecoxib in Presence of Methylacetophenone

et al. 2021

View full text Add to dashboard Cite

Aim: Green, accurate and rapid methods, namely LC–MS/MS and thin layer chromatography-densitometric methods, were developed for determination of amlodipine besylate and celecoxib in presence of its process-related impurities, 4-methylacetophenone in pure and formulated tablets. Results: LC–MS/MS was achieved on ZORBAX Eclipse Plus C18 column using methanol: aqueous solution of 5 mM formic acid (95:5 v/v). High sensitivity with low limit of detection values 0.00028, 0.00027 and 0.0003 for amlodipine, celecoxib and 4-methylacetophenone, respectively were obtained. While, thin layer chromatography-densitometric was established using methanol: water: ammonia (70: 25: 1.5, by volume). Good linearity was obtained in the range of 0.1–10 μg/band, 1–150 μg/band and 0.01–2 μg/band for amlodipine, celecoxib and 4-methylacetophenone, respectively. Conclusion: The proposed method validation was achieved according to ICH guidelines. Those methods possess advantages of being ecofriendly methods which permit their application in quality control laboratories.

show abstract

Development and validation of analytical method for simultaneous estimation of amlodipine besylate and celecoxib in pure and combined dosage form

Cited by 5 publications

References 0 publications

Development and Validation of UV-Spectroscopic Method for Simultaneous Estimation of Metformin Hydrochloride and Pravastatin Sodium

Development and Validation of UV-Spectroscopic Method for Simultaneous Estimation of Metformin Hydrochloride and Pravastatin Sodium

Development and Validation of RP-HPLC Method for Estimation of Amlodipine Besylate and Celecoxib in Pharmaceutical Formulation

Validated Green Chromatographic Methods for Determination of Amlodipine and Celecoxib in Presence of Methylacetophenone

Contact Info

Product

Resources

About