Development and validation of diagnostic markers for Fhb1 region, a major QTL for Fusarium head blight resistance in wheat

Su, Zhenqi; Jin, Sujuan; Zhang, Dadong; Bai, Guihua

doi:10.1007/s00122-018-3159-6

Cited by 68 publications

(46 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Like the 2A QTL, the QLr.spa-3B AC Cadillac leaf rust resistance mapped in the region of stripe rust resistance QTL reported in two different studies [22,24]. The most highly associated SNP marker to QLr.spa-3B, Tdurum_contig79629_538, mapped close to UMN10 [41], Xgwm389 and csSr2 markers that define the region of the slow rusting stem rust gene Sr2 [57,58]. QLr.spa-3B is in the chromosome region that restricts the development of different fungal diseases with some possibility of relationship to leaf rust resistance gene Lr27 that is known to be associated with stem rust (Sr2) and powdery mildew resistance [59].…”

Section: Qtl Detected In a Single Cultivarmentioning

confidence: 81%

“…Located on the long arm of the chromosome, this QTL is different from a major QTL, QLr.spa-2D.2, that was identified in Stettler on 2DS about 33 cM from QLr.spa-2D.1. Like QLr.spa-2D.1, the 3B QTL QLr.spa-3B was expressed in two environments albeit marginally in the one and was flanked by UMN10 [41], gwm389, bar147 and xsts3B-142. QLr.spa-4B.1 and QLr.spa-4B.2 were about 75 cM from each other on chromosome 4B.…”

Section: Carberry/ac Cadillac Populationmentioning

confidence: 98%

See 1 more Smart Citation

Mapping quantitative trait loci associated with leaf rust resistance in five spring wheat populations using single nucleotide polymorphism markers

et al. 2020

View full text Add to dashboard Cite

Growing resistant wheat (Triticum aestivum L) varieties is an important strategy for the control of leaf rust, caused by Puccinia triticina Eriks. This study sought to identify the chromosomal location and effects of leaf rust resistance loci in five Canadian spring wheat cultivars. The parents and doubled haploid lines of crosses Carberry/AC Cadillac, Carberry/Vesper, Vesper/Lillian, Vesper/Stettler and Stettler/Red Fife were assessed for leaf rust severity and infection response in field nurseries in Canada near Swift Current, SK from 2013 to 2015, Morden, MB from 2015 to 2017 and Brandon, MB in 2016, and in New Zealand near Lincoln in 2014. The populations were genotyped with the 90K Infinium iSelect assay and quantitative trait loci (QTL) analysis was performed. A high density consensus map generated based on 14 doubled haploid populations and integrating SNP and SSR markers was used to compare QTL identified in different populations. AC Cadillac contributed QTL on chromosomes 2A, 3B and 7B (2 loci), Carberry on 1A, 2B (2 loci), 2D, 4B (2 loci), 5A, 6A, 7A and 7D, Lillian on 4A and 7D, Stettler on 2D and 6B, Vesper on 1B, 1D, 2A, 6B and 7B (2 loci), and Red Fife on 7A and 7B. Lillian contributed to a novel locus QLr.spa-4A, and similarly Carberry at QLr.spa-5A. The discovery of novel leaf rust resistance QTL QLr.spa-4A and QLr.spa-5A, and several others in contemporary Canada Western Red Spring wheat varieties is a tremendous addition to our present knowledge of resistance gene deployment in breeding. Carberry demonstrated substantial stacking of genes which could be supplemented with the genes identified in other cultivars with the PLOS ONE PLOS ONE | https://doi.org/10.1371/journal.pone.0230855 April 8, 2020 1 / 22 OPEN ACCESS Citation: Bokore FE, Knox RE, Cuthbert RD, Pozniak CJ, McCallum BD, N'Diaye A, et al. (2020) Mapping quantitative trait loci associated with leaf rust resistance in five spring wheat populations using single nucleotide polymorphism markers. PLoS ONE 15(4): e0230855. https://doi.org/ 10.

show abstract

Section: Qtl Detected In a Single Cultivarmentioning

confidence: 81%

Section: Carberry/ac Cadillac Populationmentioning

confidence: 98%

Mapping quantitative trait loci associated with leaf rust resistance in five spring wheat populations using single nucleotide polymorphism markers

et al. 2020

View full text Add to dashboard Cite

show abstract

“…To distinguish between PCR failure and null alleles, a multiplex PCR protocol was employed that simultaneously amplified the respective locus in the Fhb1 interval and the transcription elongation factor 1 as reference gene in a single PCR with the given primer sequence for the PCR control gene TEF-F: 5′ATGCACCATGAGTCTCTCC; TEF-R: 5′ CTTGATGACACCAACAGCC. In addition, allelic diversity was evaluated for the Fhb1 -specific marker TaHRC-KASP (Su et al 2018 ).…”

Section: Methodsmentioning

confidence: 99%

Exploring and exploiting the genetic variation of Fusarium head blight resistance for genomic-assisted breeding in the elite durum wheat gene pool

et al. 2018

View full text Add to dashboard Cite

Key message Genomic selection had a higher selection response for FHB resistance than phenotypic selection, while association mapping identified major QTL on chromosome 3B unaffected by plant height and flowering date. Abstract Fusarium head blight (FHB) is one of the most destructive diseases of durum wheat. Hence, minimizing losses in yield, quality and avoiding contamination with mycotoxins are of pivotal importance, as durum wheat is mostly used for human consumption. While growing resistant varieties is the most promising approach for controlling this fungal disease, FHB resistance breeding in durum wheat is hampered by the limited variation in the elite gene pool and difficulties in efficiently combining the numerous small-effect resistance quantitative trait loci (QTL) in the same line. We evaluated an international collection of 228 genotyped durum wheat cultivars for FHB resistance over 3 years to investigate the genetic architecture and potential of genomic-assisted breeding for FHB resistance in durum wheat. Plant height was strongly positively correlated with FHB resistance and led to co-localization of plant height and resistance QTL. Nevertheless, a major QTL on chromosome 3B independent of plant height was identified in the same chromosomal interval as reported for the prominent hexaploid resistance QTL Fhb1, though haplotype analysis highlighted the distinctiveness of both QTL. Comparison between phenotypic and genomic selection for FHB resistance revealed a superior prediction ability of the former. However, simulated selection experiments resulted in higher selection responses when using genomic breeding values for early generation selection. An earlier identification of the most promising lines and crossing parents was feasible with a genomic selection index, which suggested a much faster short-term population improvement than previously possible in durum wheat, complementing long-term strategies with exotic resistance donors.

show abstract

“…Based on sequencing of the Fhb1 region of near-isogenic lines, fragment deletions and SNP variations of His (histidine-rich calcium binding protein) gene have been identified, and most of the materials lacking 752 bp fragments were scab resistant materials. Su et al [107] designated the His gene as TaHRC, and developed PCR molecular markers and KASP markers. These markers are more effective than UMN10 and other SSR markers developed earlier for the Fhb1.…”

Section: Molecular Marker-assisted Selectionmentioning

confidence: 99%

Breeding for the resistance to Fusarium head blight of wheat in China

Ma¹,

Zhang²,

Jinbao³

et al. 2019

Front. Agr. Sci. Eng.

View full text Add to dashboard Cite

With the changes of climate and cultivation systems, the Fusarium head blight (FHB) epidemic area in China has extended since 2000 from the reaches of the Yangtze River to the north and west winter wheat region. Breeding for FHB resistance in wheat is an effective way to control the disease. Chinese wheat breeders commenced research on FHB in the 1950s. Sumai 3, Ning 7840, Yangmai 158, Ningmai 9 and other cultivars with improved FHB resistance were developed through standard breeding methods and widely applied in production or breeding programs. In addition to intervarietal crosses, alien germplasm was used to improve FHB resistance of wheat. Addition, substitution and translocation lines with alien chromosomes or chromosome fragments were created to enhance FHB resistance. Somaclonal variation was also used to develop a FHB resistant cv. Shengxuan 3 and other cultivars with moderate resistance to FHB were released by such methods. QTL (quantitative trait loci) for FHB resistance were characterized in cultivars originating from China. The major QTL, Fhb1, was identified on chromosome 3BS in Sumai 3, Ning 894037, Wangshuibai and other Chinese resistant sources. Diagnostic molecular markers for Fhb1 have been applied in wheat breeding and breeding lines with improved FHB resistance and desirable agronomic traits have been obtained. However, breeding for FHB resistance is a long-term task, new technologies are likely to increase the efficiency of this process and better FHB resistance of new cultivars is expected to be achieved within the next decade.

show abstract

Development and validation of diagnostic markers for Fhb1 region, a major QTL for Fusarium head blight resistance in wheat

Cited by 68 publications

References 26 publications

Mapping quantitative trait loci associated with leaf rust resistance in five spring wheat populations using single nucleotide polymorphism markers

Mapping quantitative trait loci associated with leaf rust resistance in five spring wheat populations using single nucleotide polymorphism markers

Exploring and exploiting the genetic variation of Fusarium head blight resistance for genomic-assisted breeding in the elite durum wheat gene pool

Breeding for the resistance to Fusarium head blight of wheat in China

Contact Info

Product

Resources

About