Development and Validation of First Order Derivative Spectrophotometric Method for Simultaneous Estimation of Moxifloxacin and Prednisolone Acetate in Pharmaceutical Preparation

Abstract

A simple and sensitive first order derivative spectrophotometric method was developed for the simultaneous estimation of Moxifloxacin and Prednisolone Acetate in pharmaceutical dosage form. The derivative spectrophotometric method was based on the determination of both the drugs at their respective zero crossing point (ZCP). The first order derivative spectra was obtained in methanol and the determinations were made at 258 nm (ZCP of Moxifloxacin) for Prednisolone Acetate and 303 nm (ZCP of Prednisolone Acetate) for Moxifloxacin. The two drugs comply with beer's-lambert's law over the linearity range of 2-40 µg/ml for Moxifloxacin and 5-80 µg/ml for Prednisolone Acetate. The method was validated as per ICH guidelines in terms of linearity, accuracy (recovery study), precision (repeatability, intraday, interday precision), limit of detection and limit of quantification. All the validation parameters were found to be within acceptable limits.The method was found to be simple, sensitive, rapid, cost effective, accurate and precise for the routine analysis of both the drugs in pharmaceutical dosage form.

Comparative evaluation of some moxifloxacin hydrochloride tablet brands marketed in Nigeria using five different validated analytical methods

“…The method has been applied for evaluation of moxifloxacin in commercial tablet and human urine. Patel et al, (2017) worked on simultaneous spectrophotometric determination of moxifloxacin and prednisolone acetate in pharmaceutical preparation using the Q-Absorbance ratio method. In 2019, Attimard et al, worked on simultaneous determination of moxifloxacin (MOX) and flavoxate (FLX) by RP-HPLC, first derivative and ratio first derivative spectrophotometric methods using valsartan as an internal standard.…”

Determination of Levofloxacin, Norfloxacin, and Moxifloxacin in Pharmaceutical Dosage Form or Individually Using Derivative UV Spectrophotometry

“…Scanning with each monochromatic separated by a small constant wavelength difference produces the derivative spectrum. [5,6,7] The synthesis of uorinated quinolones known as uoroquinolones has opened up the eld of quinolone antibacterial chemotherapy. Fluoroquinolones have enhanced activity against a wide range of Gram-negative as well as Gram positive cocci and Enterobacteriaceae.…”