Development and validation of glycoprotein‐based native‐subunit vaccine for fish against Aeromonas hydrophila

Abstract

Aeromonas hydrophila is known to be causative agent of an infection named as Bacterial haemorrhagic septicaemia or red pest in freshwater fish. The aim of this study was to develop and validate the glycoprotein-based fish vaccine against Aeromonas hydrophila. For this aim, after identification and characterization of A. hydrophila isolates from fish farms, one A. hydrophila isolate was selected as vaccine strain. Antigenic glycoproteins of this vaccine strain were determined by Western blotting and glycan detection kit. The connection types of these glycoproteins were examined by glycoprotein differentiation kit. Two glycoproteins, molecular weights of 19 and 38 kDa, with SNA connection type were selected for use in vaccination trials. After their purification by SNA-specific lectin and size-exclusion chromatography, protection studies with purified proteins were performed. For challenge trials, four experimental fish groups were designated: Group I (with montanide), Group II (with montanide and ginseng), Group III [with Al(OH)3 ] and Group IV [with Al(OH)3 and ginseng]. The survival ratings of fish were determined, and protection was calculated as 21.56%, 29.41%, 69.83% and 78.88% in groups I, II, III and IV, respectively. In conclusion, A. hydrophila glycoproteins with Al(OH)3 and ginseng could be used as a safe and effective vaccine for fish.

Toxicity studies of drugs and chemicals in animals: an overview

“…Aeromonas veronii affects the quality of fish leading to severe losses to marketing (Cao et al, 2014). However, a glycoprotein-based nativesubunit vaccine for fish against Aeromonas hydrophila known to be causative agent of bacterial haemorrhagic septicaemia or red pest in freshwater fish was developed and validated (Ciftci et al, 2016). Hepatitis A virus and its indicator were concentrated and detected from artificial seawater using zeolite (Cormier & Janes, 2016) using the method of Saganuwan (2011).…”

Bioinformatic analysis of antivirus‐related TRIM genes in Dabry’s sturgeon Acipenser dabryanus

“…To gain information about the role of adtrim genes in response to bacterial infection, the expression pattern of adtrim genes were determine after exposure to A. hydrophila challenge. A. hydrophila , a gram‐negative bacteria, is the causative agent of an infection named Bacterial hemorrhagic septicemia, or red pest, in freshwater fish (Ciftci et al, ). After V. alginolyticus challenge, Hong Kong oyster ( Crassostrea hongkongensis ) trim9 mRNA in hemocytes showed a significant decrease at 6 hr compared with that in the control group (Liu, Li, Wang et al, ).…”

Application of Median Lethal Concentration (LC50) of Pathogenic Microorganisms and Their Antigens in Vaccine Development

“…were translated to LC1 ,whereas effective dose fifty (ED50) for Newcastle Disease vaccines was translated to ED1 in chickens [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. The method of Reed and Muench [21] as modified by Saganuwan [9] was used for LC50 determination in various laboratories.…”

“…Edwardsiella ictaluri is moderately pathogenic in Pangasionodon hypophthalamus with LC50 of 2.8 X10 4 cfu/ml and caused necrosis of liver and haemolysis [18]. Vaccination against A. hydrophila using glycoproteins (5×10 9 cfu/ml) with ginseng, provided reliable immunity in fish and rabbit [19], though the immunity may not be strong. Bacteriovorax strain H2 is relatively safe in infection [25].…”