Development and Validation of HPLC Method Along With Anti-Aging Activity for Ximenynic Acid in Santalum Album Linn.

Abstract

A new analytical approach and validation study were developed to assess the quantity of ximenynic acid (XMA) in Santalum album Linn. extract. High-performance liquid chromatography (HPLC) in reverse phase is the technique that is used in this procedure. The anti-aging properties of the medicine were tested using enzymes that inhibit hyaluronidase and elastase, respectively, in order to gather information and findings. Methanol and water were used as the mobile phase for the analysis, which was carried out using a ZORBAX SB-C18 column with a size 5 µ (4.6 x 250 mm) column size. During the course of this HPLC method validation, a number of different tests that were pertinent to specificity, linearity, accuracy, precision and robustness were carried out. The technique was tested to see whether or not it would be able to fulfill the prerequisites set out by the International Conference on Harmonization (ICH). Research conducted on anti-aging found that XMA was much more efficient than catechin at decreasing the activities of elastase and hyaluronidase. Because of its use, speed and reliability, the HPLC method that was described has the potential to be effectively utilized in industry for the standardization of herbs and phytomedicines. Because XMA has the potential to be a breakthrough anti-aging treatment, the pharmaceutical and cosmetics industries may benefit from its development.

Targeting COVID-19 through active phytochemicals of betel plant by molecular docking

“…Diabetic patients mainly use it. From ancient times people have consumed Betel leaf (Piper betle) as it helps to flush out toxins and restore normal pH and helps in increasing appetite, and it correlates with current phytochemical research (Shivatare et al, 2023;Hajare et al, 2023). Some studies also show that different combination of essential oil with Betel leaf (Piper betle) extract with antibiotics potentiates antibacterial property (Nayaka et al, 2021).…”