Development and validation of HPLC-DAD method for the simultaneous determination of amoxicillin, metronidazole and rabeprazole sodium. Application to spiked simulated intestinal fluid samples

Sabry, Suzy M.; Abdel-Hay, Mohamed H.; Belal, Tarek S.; Mahgoub, Ahmed

doi:10.1016/j.pharma.2015.04.008

Cited by 12 publications

(4 citation statements)

References 12 publications

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“…The pharmaceutical levels in the gathered mixtures were assessed using HPLC, which was performed on a Hitachi L-2200R multi-solvent delivery system (Tokyo, Japan). Acetonitrile:PBS at a ratio of 30:70 ( v / v ) (pH 7.0) was used as the mobile phase for metronidazole [ 18 ]. The wavelength and flow rate for the assay were 319 nm and 1.0 mL/min, respectively.…”

Section: Methodsmentioning

confidence: 99%

Fabrication of Drug-Eluting Polycaprolactone/poly(lactic-co-glycolic Acid) Prolapse Mats Using Solution-Extrusion 3D Printing and Coaxial Electrospinning Techniques

Chen

Lin

et al. 2021

Polymers

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We developed biodegradable drug-eluting prolapse mats using solution-extrusion 3D printing and coaxial electrospinning techniques. The mats were composed of polycaprolactone (PCL) mesh and lidocaine-, estradiol-, metronidazole-, and connective tissue growth factor (CTGF)-incorporated poly(lactic-co-glycolic acid) (PLGA) nanofibers that mimic the structure of the natural extracellular matrix of most connective tissues. The mechanical properties of degradable prolapse membrane were assessed and compared to commercial non-degradable polypropylene knitted meshes clinically used for pelvic organ prolapse (POP) repair. The release behaviors of the drug-loaded hybrid degradable membranes were also characterized. The experimental results suggest that 3D-printed PCL meshes exhibited comparable strengths to commercial POP meshes and survived through 10,000 cycles of fatigue test without breakage. Hybrid PCL meshes/PLGA nanofibrous membranes provided a sustainable release of metronidazole, lidocaine, and estradiol for 4, 25, and 30 days, respectively, in vitro. The membranes further liberated high levels of CTGF for more than 30 days. The animal tests show that the mechanical property of PCL mesh decreased with time, mainly due to degradation of the polymers post-implantation. No adverse effect of the mesh/nanofibers was noted in the histological images. By adopting solution-extrusion 3D printing and coaxial electrospinning, degradable drug-eluting membranes can be fabricated for POP applications.

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Section: Methodsmentioning

confidence: 99%

Fabrication of Drug-Eluting Polycaprolactone/poly(lactic-co-glycolic Acid) Prolapse Mats Using Solution-Extrusion 3D Printing and Coaxial Electrospinning Techniques

Chen

Lin

et al. 2021

Polymers

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“…For the determination of AM in biological fluids 6,9,13,15,18 (blood plasma, intestinal fluid, urine), multicomponent drugs, 5,6,[9][10][11] and food products 7,8,12,16 (milk, honey, chicken meat), highly efficient liquid chromatography as well as liquid chromatography coupled with tandem mass spectrometry are used. [5][6][7][8][9][10][11][12] These techniques are expensive, time-consuming and require the use of organic solvent. Furthermore, spectrophotometry, different types of voltammetry, [13][14][15][16][17][18] and titrimetry 2 as well are used for quantification of AM content in medicines and industrial wastewater.…”

Section: Structural Formula Characteristicmentioning

confidence: 99%

Development and Validation of the Simple and Sensitive Spectrophotometric Method of Amoxicillin Determination in Tablets using Sulphanilamides

Kostiv

Korkuna

Rydchuk

2020

ACSi

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A rapid, simple and sensitive spectrophotometric method for the determination of amoxicillin (AM) is described. The method is based on the previous sulphanilamide (SA) and sulphathiazole (STZ) diazotization in the medium of 0.6-0.7 M hydrochloric acid and their subsequent interaction with amoxicillin at pH = 10.5 with formation of yellow-colored azo compouds. Effective molar absorptivities at the absorbance maxima at 445 nm (SA) and 448 nm (STZ) for azo compounds were (1.74 ± 0.06) • 10 4 L × mol-1 × cm-1 and (1.97 ± 0.05) • 10 4 L × mol-1 × cm-1 , respectively. Stoichiometric ratios of the components of azo compounds were determined using continuous variations method. Based on the optimum reaction conditions, new methods were developed. These methods allow to determine the amoxicillin in concentration range 1.3-32.9 mg × mL-1 with sulphanilamide and 0.7-27.4 mg × mL-1 with sulphathiazole. The methods were successfully validated for amoxicillin determination in tablets "Amoxil".

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“…The development and adoption of new techniques for the determination of biologically active compounds in more effective combination drugs still remains one of important tasks of modern analytical and pharmaceutical chemistry. The methods of high performance liquid chromatography [6][7][8][9][10][11] and spectrophotometry [1][2][3][12][13][14][15] are often suggested for the quantitative determination of amoxicillin. The first one is a time-consuming and expensive method and the second is not a sufficiently selective and sensitive method.…”

Section: Introductionmentioning

confidence: 99%

A New Approach for the Voltammetric Determination of Amoxicillin in the Dosage Form Using Azo Coupling Reaction with Sulphanilamide

et al. 2021

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The novel method of amoxicillin (AM) determination has been developed using single-sweep polarography. The proposed method is based on the obtaining of yellow coloured azo compound due to azo coupling reaction of previous diazotized sulphanilamide (SA) (in the medium of 0.6 M hydrochloric acid) with amoxicillin at pH = 9.0 with the further reduction of the formed analytical form on a dropping mercury electrode. Voltammetric determination of amoxicillin is carried out due to the reduction peak of azo group of the obtained azo compound in the presence of 0.05 mol • L À 1 Na 2 B 4 O 7 as a background electrolyte at the potential E c p2 = À 0.55 V and potential sweep rate of 2.5 V • s À 1 . The developed voltammetric method has two linear ranges of the determined concentrations (0.05-2.0) • 10 À 5 mol • L À 1 and (0.2-1.0) • 10 À 4 mol • L À 1 and the high sensitivity: LOD without the removing of unreacted sodium nitrite is 1.1 • 10 À 6 mol • L À 1 , and 7.2 • 10 À 7 mol • L À 1 , when NaNO 2 excess is removed using urea. The developed voltammetric technique of AM determination has been approved during the analyses of tablets and oral suspension.

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Development and validation of HPLC-DAD method for the simultaneous determination of amoxicillin, metronidazole and rabeprazole sodium. Application to spiked simulated intestinal fluid samples

Cited by 12 publications

References 12 publications

Fabrication of Drug-Eluting Polycaprolactone/poly(lactic-co-glycolic Acid) Prolapse Mats Using Solution-Extrusion 3D Printing and Coaxial Electrospinning Techniques

Fabrication of Drug-Eluting Polycaprolactone/poly(lactic-co-glycolic Acid) Prolapse Mats Using Solution-Extrusion 3D Printing and Coaxial Electrospinning Techniques

Development and Validation of the Simple and Sensitive Spectrophotometric Method of Amoxicillin Determination in Tablets using Sulphanilamides

A New Approach for the Voltammetric Determination of Amoxicillin in the Dosage Form Using Azo Coupling Reaction with Sulphanilamide

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