Development and Validation of HPTLC Method for Simultaneous Estimation of Terbinafine Hydrochloride and Mometasone Furoate in Combined Dosage Form

Patel, Mehul; Patel, Heta D

doi:10.4067/s0717-97072016000200021

Cited by 19 publications

(12 citation statements)

References 8 publications

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“…nomenclature: 3-(2-methoxyethyl) 5-(2E)-3phenylprop-2-en-1-yl 2,6-dimethyl-4-(3-nitrophenyl)-1,4dihydropyridine-3,5-dicarboxylate Literature review suggest few RP-HPLC, HPTLC, spectroscopic, stability indicating HPLC determinations were performed. [2][3][4][5][6][7][8][9][10][11][12][13][14][15] The aim of the present study is to develop a simple, precise, accurate, sensitive HPLC method for the determination. After, doing in depth study in the present research work, it was found that the present research work is having various advantages over the previous work.…”

Section: Iupacmentioning

confidence: 99%

Development and Validation of Stability Indicating RP-HPLC Method for Estimation of Cilnidipine

Tiwari

Shirsat

Kulkarni³

2020

J. Drug Delivery Ther.

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Cilnidipine is one of the dihydropyridine calcium antagonists. It was created combinedly by Fuji Viscera Pharmaceutical Company, Ajinomoto and Japan and was approved in the year 1995. Cilnidipine acts on N-type calcium channel where exist the end of sympathetic nerve in addition to common L-type calcium channel like that of other calcium antagonists. China, Japan, India, Korea and several other countries approved this drug. The objective of the method validation is to demonstrate whether the method was suited for the intended purpose. The method was validated as per the ICH guidelines. The method was validated for linearity, precision (repeatability, intermediate precision), accuracy, specificity, robustness, limit of detection and limit of quantification. Cosmosil (4.6 X 250mm, 5 μ) column was used for separation. The selected wavelength for Cilnidipine was 241 nm. The mobile phase consists Methanol: Potassium dihydrogen phosphate buffer (50:50). Flow rate was delivered at 1.0 mL/min. Appropriate dilutions of standard stock solutions were prepared as per the get desired concentrations in the range of 100-500 mcg/ml. The RT obtained was 4.8165 minutes. Keywords: Cilnidipine, UV spectroscopy, RP-HPLC, ICH

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Section: Iupacmentioning

confidence: 99%

Development and Validation of Stability Indicating RP-HPLC Method for Estimation of Cilnidipine

Tiwari

Shirsat

Kulkarni³

2020

J. Drug Delivery Ther.

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“…A mixture of ND and MF are available in cream dosage firms which topically applied for the treatment of dermatoses. Literature surveys revealed that ND is not official in any pharmacopoeia [3] while MF is an official drug in European Pharmacopoeia [4].The literature survey revealed several analytical methods have been reported for the determination of ND alone or in combinations with other drugs including, spectrophotometry [5,6], HPTLC [7,8]HPLC [9][10][11], Different methods were reported for determination of MF alone or in combinations with other drugs including, spectrophotometry, [12][13][14],TLC [8,15]and HPLC [16][17][18][19][20][21][22] To the best of author's knowledge there is only one method has been reported for the determination of ND and MF in combinations using HPTLC technique [23]. The work in this paper was aimed to develop two selective, accurate and precise chromatographic methods for the simultaneous determination of ND and MF in topical cream.…”

Section: Introductionmentioning

confidence: 99%

Simple development and validation of RP-HPLC and TLC- densitometric methods for the simultaneous determination of nadifloxacine and mometasone furoate in their binary mixture

Abdallaha

Abdel-Megiede

Abdelrahman

et al. 2020

Journal of advanced Biomedical and Pharmaceutical Sciences

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The present work is concerned with development, optimization and validation of two chromatographic methods for the simultaneous determination of nadifloxacine (ND) and mometasone furoate (MF). The first developed method was RP-HPLC depended on chromatographic separation using Phenyl-hexyl column and a mobile phase composed of acetonitrile: acidified water with orthophosphoric acid up to (pH 2.5 ± 0.1) in the proportion of (65: 35 v/v) pumped at a flow rate of 1.25 mL min −1. All measurements were performed with UV detection at 254 nm. The second method was TLC-densitometry, chromatographic separation was established on aluminum TLC plates pre-coated with silica gel GF254 as the stationary phase and chloroform: methanol: hexane: ethyl acetate: acetic acid (9: 1: 3: 3: 0.1, by volume) as the mobile phase followed by densitometric measurement of the separated bands at 254 nm. Validation of the suggested methods was successfully applied with respect to ICH guidelines. The proposed chromatographic methods were used to determine both drugs binary mixture in pure form and dosage form. The proposed methods give good linearity in the range 0.5-5.0 μg/mL and 0.5-40 µg/spot for HPLC and HPTLC methods, respectively. While MF standard solutions in the range 0.2-1.2 μg/mL and 0.5-3.8 µg/ spot for HPLC and HPTLC methods, respectively. The obtained results were statistically compared with those achieved by the reported methods, showing no significant difference with respect to accuracy and precision at p = 0.05.

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“…It was found that UV spectroscopic, stability-indicating RP-HPLC, HPLC, and bioanalytical methods have been reported for analysis of these two drugs in combination [5][6][7][8][9][10][11][12]. However, there is only one paper reported for the analysis of the combination by high-performance thin-layer chromatography (HPTLC) [13].…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of High-Performance Thin-Layer Chromatography Method for Simultaneous Estimation of Nebivolol Hydrochloride and Cilnidipine

Ghante¹,

Akhade²,

Gota³

et al. 2019

Asian J Pharm Clin Res

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Objective: The proposed method describes method development and validation of nebivolol hydrochloride and cilnidipine in combined pharmaceutical tablet dosage form by high-performance thin-layer chromatography (HPTLC) having adequate specificity, sensitivity, and reproducibility. Methods: Nebivolol hydrochloride and cilnidipine drug combination is used for the treatment of hypertension. Precoated aluminum plates with silica gel 60 F254 (E-Merck, Germany) were used for the chromatographic separation which was carried using chloroform:glacial acetic acid:methanol, in 8.5:1:0.5 (v/v/v) as a mobile phase. HPTLC separation of two drugs was carried by densitometric measurement at 270 nm. Reults: The drugs were satisfactorily resolved with retardation factor values of 0.0.29±0.008 and 0.69±0.007 for nebivolol hydrochloride and cilnidipine, respectively. The method was found to be linear in the range of 100–1000 ng/spot and 50–500 ng/spot for nebivolol hydrochloride and cilnidipine, respectively. The correlation coefficient was found to be 0.989 and 0.996 for nebivolol hydrochloride and cilnidipine, respectively. Limit of detection and limit of quantitation values were 16.395 ng/band and 49.681 ng/band and 31.788ng/band and 96.328 ng/band, respectively. The mean recovery was found to be 100.570–101.936 and 100.269–101.333 for nebivolol hydrochloride and cilnidipine, respectively. The intra- and inter-day precision was found to be within the limit. Conclusion: A simple, accurate, precise, and sensitive HPTLC method has been developed and validated in combined pharmaceutical tablet dosage form for simultaneous estimation of nebivolol hydrochloride and cilnidipine.

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Development and Validation of HPTLC Method for Simultaneous Estimation of Terbinafine Hydrochloride and Mometasone Furoate in Combined Dosage Form

Cited by 19 publications

References 8 publications

Development and Validation of Stability Indicating RP-HPLC Method for Estimation of Cilnidipine

Development and Validation of Stability Indicating RP-HPLC Method for Estimation of Cilnidipine

Simple development and validation of RP-HPLC and TLC- densitometric methods for the simultaneous determination of nadifloxacine and mometasone furoate in their binary mixture

Development and Validation of High-Performance Thin-Layer Chromatography Method for Simultaneous Estimation of Nebivolol Hydrochloride and Cilnidipine

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