Development and validation of KASP markers for the greenbug resistance gene Gb7 and the Hessian fly resistance gene H32 in wheat

Abstract

Greenbug and Hessian fly are important pests that decrease wheat production worldwide. We developed and validated breeder-friendly KASP markers for marker-assisted breeding to increase selection efficiency. Greenbug (Schizaphis graminum Rondani) and Hessian fly [Mayetiola destructor (Say)] are two major destructive insect pests of wheat (Triticum aestivum L.) throughout wheat production regions in the USA and worldwide. Greenbug and Hessian fly infestation can significantly reduce grain yield and quality. Breeding for resistance to these two pests using marker-assisted selection (MAS) is the most economical strategy to minimize losses. In this study, doubled haploid lines from the Synthetic W7984 × Opata M85 wheat reference population were used to construct linkage maps for the greenbug resistance gene Gb7 and the Hessian fly resistance gene H32 with genotyping-by-sequencing (GBS) and 90K array-based single nucleotide polymorphism (SNP) marker data. Flanking markers were closely linked to Gb7 and H32 and were located on chromosome 7DL and 3DL, respectively. Gb7-linked markers (synopGBS773 and synopGBS1141) and H32-linked markers (synopGBS901 and IWB65911) were converted into Kompetitive Allele Specific PCR (KASP) assays for MAS in wheat breeding. In addition, comparative mapping identified syntenic regions in Brachypodium distachyon, rice (Oryza sativa), and sorghum (Sorghum bicolor) for Gb7 and H32 that can be used for fine mapping and map-based cloning of the genes. The KASP markers developed in this study are the first set of SNPs tightly linked to Gb7 and H32 and will be very useful for MAS in wheat breeding programs and future genetic studies of greenbug and Hessian fly resistance.

Genotyping by Sequencing in Almond: SNP Discovery, Linkage Mapping, and Marker Design

“…Among the many technologies that can be used to assay SNPs, allele-specific Kompetitive Allele Specific PCR (KASP) assays (LGC Genomics, Teddington, United Kingdom) are now widely used in plant genetics and breeding ( e.g. , Babiker et al 2016 ; Rasheed et al 2016 ; Tan et al 2017 ).…”

Improving Dual-Purpose Winter Wheat in the Southern Great Plains of the United States

“…During the last two decades, great progress has been made in identifying Hessian fly biotypes, mapping and transferring resistant genes, characterizing resistant germplasm, and developing genetic markers for MAS [54][55][56][57][58]. To date, at least 18 Hessian fly biotypes have been classified, and at least 34 Hessian fly-resistant genes have been described in wheat and its relatives [57]. A few major resistant genes have been precisely mapped and effectively used in breeding wheat cultivars resistant to the Hessian fly using MAS [57,58].…”

“…To date, at least 18 Hessian fly biotypes have been classified, and at least 34 Hessian fly-resistant genes have been described in wheat and its relatives [57]. A few major resistant genes have been precisely mapped and effectively used in breeding wheat cultivars resistant to the Hessian fly using MAS [57,58]. As selecting genotypes resistant to the Hessian fly is impractical in the field, MAS will be the most effective approach in breeding wheat cultivars resistant to the fly, and it has become a common practice in several wheat breeding programs in the Southern Great Plains of the United States.…”

Developing KASP Markers on a Major Stripe Rust Resistance QTL in a Popular Wheat TAM 111 Using 90K Array and Genotyping‐by‐Sequencing SNPs

“…Amplification was performed using the ABI 7500 instrument (Applied Biosystems) following protocols in Tan et al (2017aTan et al ( , 2017b. A total of six consistent and representative SNPs tightly linked to QYr.tamu-2B were converted to the KASP platform.…”

“…Protocols for the preparation and running of KASP reactions are given in the KASP manual (http://www.kbioscience.co.uk). Amplification was performed using the ABI 7500 instrument (Applied Biosystems) following protocols in Tan et al (2017aTan et al ( , 2017b. The KASP assay was validated to detect and distinguish resistant and susceptible alleles for the major QTL on 2BL following the same procedure of Tan et al (2017aTan et al ( , 2017b.…”