Development and validation of LC-MS/MS assay for the determination of Butoconazole in human plasma: Evaluation of systemic absorption following topical application in healthy volunteers

Nouman, Eman G.; Al‐Ghobashy, Medhat A.; Lotfy, Hayam M.

doi:10.1016/j.bfopcu.2017.04.003

Cited by 4 publications

(2 citation statements)

References 14 publications

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“…The extraction recovery and process efficiency were used to assess the ability of the sample extraction method to consistently recover the analyte and IS. The former represents the true recovery value, which is not affected by the matrix effect, whereas the latter shows the overall process efficacy [ 22 ]. The matrix effect was calculated by dividing the mean peak areas of ARV-110 spiked in the extracted blank plasma (set 2) by the peak area of the analyte in acetonitrile (set 1).…”

Section: Methodsmentioning

confidence: 99%

Development of an LC-MS/MS Method for ARV-110, a PROTAC Molecule, and Applications to Pharmacokinetic Studies

et al. 2022

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ARV-110, a novel proteolysis-targeting chimera (PROTAC), has been reported to show satisfactory safety and tolerability for prostate cancer therapy in phase I clinical trials. However, there is a lack of bioanalytical assays for ARV-110 determination in biological samples. In this study, we developed and validated an LC-MS/MS method for the quantitation of ARV-110 in rat and mouse plasma and applied it to pharmacokinetic studies. ARV-110 and pomalidomide (internal standard) were extracted from the plasma samples using the protein precipitation method. Sample separation was performed using a C18 column and a mobile phase of 0.1% formic acid in distilled water–0.1% formic acid in acetonitrile (30:70, v/v). Multiple reaction monitoring was used to quantify ARV-110 and pomalidomide with ion transitions at m/z 813.4 → 452.2 and 273.8 → 201.0, respectively. The developed method showed good linearity in the concentration range of 2–3000 ng/mL with acceptable accuracy, precision, matrix effect, process efficiency, and recovery. ARV-110 was stable in rat and mouse plasma under long-term storage, three freeze-thaw cycles, and in an autosampler, but unstable at room temperature and 37 °C. Furthermore, the elimination of ARV-110 via phase 1 metabolism in rat, mouse, and human hepatic microsomes was shown to be unlikely. Application of the developed method to pharmacokinetic studies revealed that the oral bioavailability of ARV-110 in rats and mice was moderate (23.83% and 37.89%, respectively). These pharmacokinetic findings are beneficial for future preclinical and clinical studies of ARV-110 and/or other PROTACs.

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Section: Methodsmentioning

confidence: 99%

Development of an LC-MS/MS Method for ARV-110, a PROTAC Molecule, and Applications to Pharmacokinetic Studies

et al. 2022

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“…A literature survey revealed several other methods proposed for the determination of BTC from various matrices such as pure form, human plasma and urine, rat plasma and tissue, cream, and water samples. These methods include ultraperformance liquid chromatography (UPLC) with UV detection, reversed-phase liquid chromatography (RPLC) with UV detection, high-performance thin-layer chromatography (HPTLC)-densitometry, fabric phase sorptive extraction-HPLC with photodiode array detection (FPSE-HPLC-PDA), microextraction by packed sorbent-HPLC (MEPS-HPLC-DAD), liquid chromatography-tandem mass spectrometry (LC-MS/MS), , capillary electrophoresis (CE), and spectrophotometry . Since BTC is a chiral molecule, liquid chromatography-electrospray ionization coupled with tandem mass spectrometry (LC-ESI-MS/MS) was proposed to detect its enantiomers. , Although these methods are reliable and generate accurate results, they have several limitations such as high equipment costs, the consumption of large amounts of solvents, and laborious processes of extraction and separation of target analytes.…”

Section: Introductionmentioning

confidence: 99%

N-Methylfulleropyrrolidine-Based Multimode Sensor for Determination of Butoconazole Nitrate

Tuchiu

Staden

et al. 2022

ACS Omega

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A multimode sensor (a sensor responding simultaneously to more than one mode, e.g., stochastic mode, amperometric mode, voltammetric mode) based on graphite paste modified with N-methylfulleropyrrolidine was proposed for the determination of butoconazole nitrate in its pharmaceutical formulation. The stochastic mode and square wave voltammetry mode were applied for the determinations. Both the stochastic mode and square wave voltammetry mode were applied for a qualitative and quantitative assay of butoconazole nitrate. The sensor can be used between 1.68 × 10 −6 and 1.68 × 10 4 μmol L −1 when the stochastic mode is used and between 0.168 and 16.80 μmol L −1 when the square wave voltammetry mode is used. The multimode sensor was reliably used for the determination of butoconazole nitrate in its pharmaceutical formulation, Gynofort cream, the recorded recoveries being higher than 99.00%, with RSD (%) values of lower than 2.00%.

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Extract of radish (R. Sativus Linn) promotes anti-atherosclerotic effect using urine metabolomics in ApoE−/− mice

Hwang

Shin

et al. 2021

Journal of Functional Foods

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Development and validation of LC-MS/MS assay for the determination of Butoconazole in human plasma: Evaluation of systemic absorption following topical application in healthy volunteers

Cited by 4 publications

References 14 publications

Development of an LC-MS/MS Method for ARV-110, a PROTAC Molecule, and Applications to Pharmacokinetic Studies

Development of an LC-MS/MS Method for ARV-110, a PROTAC Molecule, and Applications to Pharmacokinetic Studies

N-Methylfulleropyrrolidine-Based Multimode Sensor for Determination of Butoconazole Nitrate

Extract of radish (R. Sativus Linn) promotes anti-atherosclerotic effect using urine metabolomics in ApoE−/− mice

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