Development and Validation of Methamphetamine Analysis Methods in Dried Blood Spot Sample Using Gas Chromatography – Mass Spectrometry

Abstract

Methamphetamine or known as sabu is one of the amphetamine groups that give a very strong central nervous system stimulant effect. Nowadays, the method used to test the specimens to prove a person who had methamphetamine, for example is urine and blood. This research was aimed to develop methamphetamine analysis method in Dried Blood Spot (DBS). This research used non-invasive bio sampling method namely DBS using Gas Chromatography-Mass Spectrometry (GC-MS) because it is suitable for compounds that are stable to high temperatures and present in small levels in the body. This research started from optimization of gas chromatography condition-optimization mass spectrometry, optimization of sample preparation method from DBS, until validation of bio analysis method. The optimum chromatographic conditions were MS-5 capillary DB columns with a length of 30 m; 0.25 mm inner diameter; Helium gas phase 99.999%; 1.0 mL / min flow rate; detection of MS at m / z value 58.00; 91.00; and 77,00 with ephedrine HCl as an internal standard. Sample preparation using liquid-liquid micro-extraction method (LLM) using methanol solvent then residue dried under the flow of N2 gas and reconstituted with 50 μL ethyl acetate. The method obtained was linear in the concentration range 1.750-35.000 ng / mL with r > 0.9800. (y =-0.0001 + 0.0004x withr = 0.9987). The validation results of the methamphetamine analysis method performed met the validation requirements based on the EMEA Bio analytical Guideline of 2011. This method can be applied in the detection and analysis of amphetamine in drug abuse.