Development and validation of miRNA based method for rapid identification of offal meats in processed chicken meat products

Vishnuraj, M. R.; Devatkal, Suresh; Vaithiyanathan, S.; Kumar, Renuka; Mendiratta, S. K.

doi:10.1016/j.foodcont.2020.107593

Cited by 11 publications

(3 citation statements)

References 30 publications

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“…Meat authentication techniques are based on the identification of targeted analytes, namely biomarkers, or the identification of a chemical analyte pattern unique for the tested meat species. Classification of these techniques based on the group of the analytes to be detected includes DNA-based (classical techniques and genomics) (Dirong et al, 2021;Azad, 2023), RNA-based (transcriptomics) (Vishnuraj, Devatkal, Vaithiyanathan, Uday Kumar & Mendiratta, 2021), proteins-based (proteomics) (Stachniuk, Sumara, Montowska & Fornal, 2021;Suratno, Windarsih, Warmiko, Khasanah, Indrianingsih & Rohman, 2023), metabolites-based (metabolomics) (Zhang, Chen, Xie, Wang & Pan, 2021;Harlina, Maritha, Musfiroh, Huda, Sukri & Muchtaridi, 2022;Suratno, 2023), lipids-based (lipidomics) (Harlina et al, 2022;Jia, Di & Shi, 2023) and glycans-based (glycomics) based techniques (Shi et al, 2019;Chia et al, 2022).…”

Section: Analytical Approaches On Meat Adulteration Controlmentioning

confidence: 99%

Detection of Authentication of Meat Products by Low Cost Closed-tube Molecular Methods

Laliotis,

Batrinou,

Giannakourou

et al. 2023

JFR

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Nowadays adulteration of meat products, especially of ground meat products which form an easy case scenario for implementing adulteration practices due to their structure and texture, emerges a critical issue of raised concern threatening fair trade, food quality and consumers’ health and protection. Food authentication testing is the tool to address this kind of fraud. There is several analytical methodologies applied for meat authentication targeting at different biomarkers and using a variety of analytical techniques. However, the applied methodologies should exhibit suitable performance characteristics such as reliability, sensitivity, reproducibility and availability in order to be fit for purpose. During the last 20 years, amplification tests have emerged as an important diagnostic tool, not only for clinical applications, but also for food quality and safety. It was urgent to develop molecular techniques fast and sensitive. The introduction of new DNA technologies has facilitated the ease and accuracy of of methods for fraud detection. The closed-tube methods of Loop-mediated isothermal amplification (LAMP) and Gold Nanoparticles linked with oligonucleotides used as molecular probes are well known for their robust and highly sensitive and specific amplification of target DNA. Moreover, these techniques are rapid, low-cost diagnostics and available on site. This review provides a comprehensive overview of the molecular methods developed that can be applied for investigating ground meat adul-teration and focuses on the advantages of the rapid closed tube methods that can yield color results interpreted with the naked eye. The application of such time- and cost-effective molecular tools in the food market is proposed to provide a first-level filter for meat adulterated products, serving as a complementary tool to the more in-depth -omics approach.

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Section: Analytical Approaches On Meat Adulteration Controlmentioning

confidence: 99%

Detection of Authentication of Meat Products by Low Cost Closed-tube Molecular Methods

Laliotis,

Batrinou,

Giannakourou

et al. 2023

JFR

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“…In the recent past, Vishnuraj et al [88] developed an innovative miRNA-based approach to detect the presence of liver, heart, and gizzard in chicken skeletal meat. This study performed deep sequencing on miRNAs obtained from the different chicken body parts.…”

Section: Transcriptomics-based Approachesmentioning

confidence: 99%

Omics-Based Analytical Approaches for Assessing Chicken Species and Breeds in Food Authentication

et al. 2021

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Chicken is known to be the most common meat type involved in food mislabeling and adulteration. Establishing a method to authenticate chicken content precisely and identifying chicken breeds as declared in processed food is crucial for protecting consumers’ rights. Categorizing the authentication method into their respective omics disciplines, such as genomics, transcriptomics, proteomics, lipidomics, metabolomics, and glycomics, and the implementation of bioinformatics or chemometrics in data analysis can assist the researcher in improving the currently available techniques. Designing a vast range of instruments and analytical methods at the molecular level is vital for overcoming the technical drawback in discriminating chicken from other species and even within its breed. This review aims to provide insight and highlight previous and current approaches suitable for countering different circumstances in chicken authentication.

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“…Currently, there is no standard or universal method to identify frauds; therefore, the primary goal is to identify specific biomarkers capable of revealing and validating potential food adulterations. At the same time, it is crucial to develop methods of analysis for biomarkers recognized by regulatory agencies as unique, reproducible, and effective target biomolecules to be determined [ 22 , 23 ]. miRNAs have the potential to become fundamental biomarkers that would allow food testing for the presence of undeclared species in meat-based products, ensuring highly reliable results.…”

Section: Introductionmentioning

confidence: 99%

miRNAs as Biomolecular Markers for Food Safety, Quality, and Traceability in Poultry Meat—A Preliminary Study

Baraldo,

Buzzoni,

Pasti

et al. 2024

Molecules

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In this study, the expression and abundance of two candidate chicken (Gallus gallus; gga) microRNAs (miRNAs, miR), gga-miR-21-5p (miR-21) and gga-miR-126-5p (miR-126), have been analyzed in order to identify biomarkers for the traceability and quality of poultry meat. Two breeds of broiler chickens were tested: the most common Ross308 (fast-growing) and the high-quality Ranger Gold (slow-growing). A preliminary analysis of the two miRNAs expressions was conducted across various tissues (liver, lung, spleen, skeletal muscle, and kidney), and the three tissues (lung, spleen, and muscle) with a higher expression were chosen for further analysis. Using quantitative reverse transcription polymerase chain reaction (RT-qPCR), the expression of miRNAs in the three tissues of a total of thirteen animals was determined. The results indicate that miR-126 could be a promising biomarker for the lung tissue in the Ranger Gold (RG) breed (p < 0.01), thus suggesting a potential applicability for tracing hybrids. RG exhibits a significantly higher miR-126 expression in the lung tissue compared to the Ross308 broilers (R308), an indication of greater respiratory capacity and, consequently, a higher oxidative metabolism of the fast-growing hybrid. During sampling, two R308 broilers presented some anomalies, including airsacculitis, hepatic steatosis, and enlarged spleen. The expression of miR-126 and miR-21 was compared in healthy animals and in those presenting anomalies. Chickens with airsacculitis and hepatic steatosis showed an up-regulation of miR-21 and miR-126 in the most commercially valuable tissue, the skeletal muscle or breast (p < 0.05).

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Development and validation of miRNA based method for rapid identification of offal meats in processed chicken meat products

Cited by 11 publications

References 30 publications

Detection of Authentication of Meat Products by Low Cost Closed-tube Molecular Methods

Detection of Authentication of Meat Products by Low Cost Closed-tube Molecular Methods

Omics-Based Analytical Approaches for Assessing Chicken Species and Breeds in Food Authentication

miRNAs as Biomolecular Markers for Food Safety, Quality, and Traceability in Poultry Meat—A Preliminary Study

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