Development and Validation of Q-Absorbance Ratio Method for Simultaneous Estimation of Valsartan and Nifedipine in Synthetic Mixture

Patel, Chiragkumar B; Patel, Satish A.

doi:10.7897/2230-8407.07111

Cited by 7 publications

(6 citation statements)

References 1 publication

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“…Upon reviewing the literature, it has been divulged that binary mixtures of AMX and MET were previously determined using hydrophilic interaction chromatography (HILIC) [ 21 ], UV spectroscopy [ 22 , 23 ] and HPLC [ 24 ]. Also, AMX and MET but this time with famotidine (FAM), an HPTLC report for their analysis in simulated gastric juice was found [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

An eco-friendly liquid chromatographic analysis of the triple therapy protocol of amoxicillin, metronidazole and vonoprazan for H. Pylori eradication: application to combined dosage forms and simulated gastric fluid

Mahgoub,

Ragab,

Tarek

et al. 2024

BMC Chemistry

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Helicobacter pylori has a big sway when peptic ulcers are concerned. For its eradication, different protocols have been approved. Among which, the tripartite therapy protocol which embraces vonoprazan as potassium competitive acid blocker in combination with amoxicillin and metronidazole as antibiotics. An environmentally benign HPLC method is addressed in order to simultaneously determine amoxicillin (AMX), metronidazole (MET) and vonoprazan (VPZ) in bulk powder and combined tablet mixture. Full separation of AMX, MET and VPZ is accomplished using C8 column, and a gradient mobile phase system, composed of methanol and phosphate buffer of a pH value of 5. Fine linearity in the concentration ranges 50–600 µg mL−1 amoxicillin, 50–400 µg mL−1 metronidazole and 10–100 µg mL−1 vonoprazan was denoted by the high correlation coefficient (0.9999). The method accuracy and precision are confirmed upon analyzing AMX, MET and VPZ triple therapy not only in their synthetic mixtures and combined tablet mixtures but also in their combined tablet mixtures in simulated gastric fluid. AMX, MET and VPZ triple therapy could be routinely analyzed in QC labs, in case of being co-formulated, using the presented method. Three different assessment tools were adopted revealing the benign environmental impact of presented method.

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Section: Introductionmentioning

confidence: 99%

An eco-friendly liquid chromatographic analysis of the triple therapy protocol of amoxicillin, metronidazole and vonoprazan for H. Pylori eradication: application to combined dosage forms and simulated gastric fluid

Mahgoub,

Ragab,

Tarek

et al. 2024

BMC Chemistry

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“…An extensive literature survey revealed that few analytical methods such as HPLC, HPTLC, and UV spectrophotometric are available on individual drugs and in combination with other drugs. Literature reveals that only a many few methods like Q‐absorbance UV [20], HPLC [21, 22] method, and so forth are reported for their simultaneous estimation. Hence, the research study focuses on the development and validation of a sensitive, accurate, precise, and simple HPTLC method for simultaneous estimation of IVA and CAR in bulk and pharmaceutical formulation.…”

Section: Introductionmentioning

confidence: 99%

Quality by Design approach for a planar chromatographic method for simultaneous estimation of Ivabradine Hydrochloride and Carvedilol in pharmaceutical formulation

Chadha

Parmar

Shah

et al. 2023

Separation Science Plus

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Box-Behnken design was applied for the development of a novel, precise, simple, and accurate high-performance thin-layer chromatography method for simultaneous estimation of mentioned drugs in the formulation. Optimization was performed using Quality by Design. Principles of risk assessment followed by application of design for optimization of chromatographic conditions to determine critical parameters: volume of hexane, chamber saturation time, solvent front and their effects on critical method attributes: R f values of both drugs. Optimized conditions were determined through Derringer's desirability approach of the multi-criteria decision-making technique. Good resolution using optimized mobile phase, hexane:ethyl acetate:ammonium acetate in methanol (5.1:3:2) with R f values 0.34 and 0.67 for Ivabradine Hydrochloride and Carvedilol, respectively, was obtained. The method was validated as per International Council on Harmonization having a linear range of 1000-5000 ng/band of Ivabradine and 1250-6250 ng/band for Carvedilol, respectively. %Recovery was in range of 99.23-101.3 and 99.8-100.5, respectively. Precision and robustness were found to be in an acceptable range. Thus, Quality by Design based method development assisted in making design space with knowledge of all method performance characteristics with a better understanding of high-performance thin-layer chromatography. The Analytical GREEnness profile was assessed using AGREE tool with an overall reliable score of 0.68.

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“…Numerous analytical methods for determining ESZ have been developed either alone, or in mixture with other https://aijpms.journals.ekb.eg/ drugs in pharmaceuticals and in biological fluids 27,28 . Limited methods were published for the simultaneous estimation of ASP and ESZ as spectrophotometric 29 , HPTLC 30 , UPLC 31 and HPLC methods 32 . Green analytical chemistry was announced to minimize the hazardous of procedures on workers and the environment.…”

Section: Introductionmentioning

confidence: 99%

Green UV Spectrophotometric Methods for Simultaneous Determination of Aspirin and Esomeprazole in Laboratory Prepared Capsules

Fouad,

Rashed,

Hosameldin

2023

Azhar International Journal of Pharmaceutical and Medical Scien

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Simple, green and accurate UV spectrophotometric methods were established aiming for simultaneous estimation of aspirin and esomeprazole without prior separation. These methods are zero order absorption, ratio subtraction, mean centering and absorbance subtraction. In zero order method esomeprazole was measured at 302.0 nm at which aspirin has no absorbance while aspirin was determined by a ratio subtraction at 225.0 nm after deduction of overlapping caused by esomeprazole. In mean centering, the mean centered values were estimated at 228.0 nm and 262.0 nm for aspirin and esomeprazole respectively. In absorbance subtraction method the two drugs intersect at isoabsorptive point at 237.0 nm and esomeprazole spectrum is further prolonged where aspirin has no absorbance. The isoabsorptive point can be used for their determination using calculated factor called absorbance factor. These methods are effective for estimation of aspirin and esomeprazole in dosage form. Moreover, the greenness of the suggested methods was evaluated demonstrating minimum hazardous effect on the environment.

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Development and Validation of Q-Absorbance Ratio Method for Simultaneous Estimation of Valsartan and Nifedipine in Synthetic Mixture

Cited by 7 publications

References 1 publication

An eco-friendly liquid chromatographic analysis of the triple therapy protocol of amoxicillin, metronidazole and vonoprazan for H. Pylori eradication: application to combined dosage forms and simulated gastric fluid

An eco-friendly liquid chromatographic analysis of the triple therapy protocol of amoxicillin, metronidazole and vonoprazan for H. Pylori eradication: application to combined dosage forms and simulated gastric fluid

Quality by Design approach for a planar chromatographic method for simultaneous estimation of Ivabradine Hydrochloride and Carvedilol in pharmaceutical formulation

Green UV Spectrophotometric Methods for Simultaneous Determination of Aspirin and Esomeprazole in Laboratory Prepared Capsules

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