Development and Validation of Quantitative Analysis Method for Phenanthrenes in Peels of the Dioscorea Genus

Abstract

Phenanthrenes are bioactive phenolic compounds found in genus Dioscorea, in which they are distributed more in peel than in flesh. Recent studies on phenanthrenes from Dioscorea sp. peels have revealed the potential for valuable biomaterials. Herein, an analytical method using highperformance liquid chromatography (HPLC) for quantitation of bioactive phenanthrenes was developed and validated. The calibration curves were obtained using the phenanthrenes (1-3) previously isolated from Dioscorea batatas concentrations in the range of 0.625-20.00 μg/ml with a satisfactory coefficient of determination (R 2 ) of 0.999. The limit of detection (LOD) and the limit of quantification (LOQ) values of the isolated phenanthrenes ranged from 0.78-0.89 and 2.38-2.71 μg/ml, respectively. The intraday and interday precision ranged from 0.25-7.58%. The recoveries of the isolated phenanthrenes were from 95 to 100% at concentrations of 1.25, 2.5, and 5.0 μg/ml. Additionally, phenanthrenes (1-3) were found in all investigated peel extracts. Hence, the developed method was encouraging for the quantitative analysis of phenanthrenes in genus Dioscorea.

Quantification of Allantoin in Yams (Dioscorea sp.) Using a 1H NMR Spectroscopic Method

“…Yams (Dioscorea spp.) are economically valued herbs and are widely used for promoting health and folk medication as well [25]. Reported biological studies revealed that D. quinqueloba extract possesses effects on cardiovascular and inflammatory skin diseases [26,27].…”

Dioscorea spp.: Bioactive Compounds and Potential for the Treatment of Inflammatory and Metabolic Diseases

“…Phenanthrene is a group of polycyclic aromatic hydrocarbons naturally occurring in various plants. Kim et al [ 32 ] quantified the contents of three phenanthrenes 2,7-dihydroxy-4,6-dimethoxyphenanthrene, 6,7-dihydroxy-2,4-dimethoxyphenanthrene, and batatasin in the pericarp of D. batatas , D. polystachya , D. quinqueloba , and D. bulbifera . The highest content of 2,7-dihydroxy-4,6-dimethoxyphenanthrene was 173.69 μg/g in D. quinqueloba .…”