Development and validation of rapid and sensitive LC methods with PDA and fluorescence detection for determination of piribedil in rat plasma and brain tissues and their pharmacokinetic application

Abstract

Simple, selective and sensitive high-performance liquid chromatographic (HPLC) bioanalytical methods using fluorescence (FL) and photodiode array (PDA) detectors were developed and validated for determination of piribedil (PBD), an anti-Parkinson's drug, in rat plasma and brain samples, with telmisartan as internal standard (IS). Protein precipitation technique was used to extract PBD from both biological matrices. Chromatographic separation was achieved on a Phenomenex Kinetex C end-capped column (250 × 4.6 mm, 5 μm), with 38:62 v/v acetonitrile and ammonium acetate buffer (pH 5.0) as mobile phase at 1.0 mL/min flow rate. Linear response in the concentration ranges 5-300 and 150-3000 ng/mL in plasma, and 15-900 and 450-9000 ng/g in brain tissue were achieved in FL and PDA detectors, respectively. The chromatograms were extracted at 239 nm in case of PDA detection and at excitation wavelength of 239 nm and emission wavelength of 385 nm in case of FL detection. FL detection was found to be more sensitive compared with PDA detection. The developed methods were successfully employed in determining the plasma time course, brain distribution and the pharmacokinetic parameters of PBD following intravenous bolus administration of the drug in male Wistar rats.

Simple and rapid LC-MS/MS method for determination of Piribedil in human plasma

“…A non-ergot dopamine receptor agonist with interesting pharmacology in that it is a Ɒ2 and Ɒ3 receptor agonist in addition to a 2-adrenoceptor agonist (Regnier et al 1968;Gobert et al 2003). Several effects on dopamine-related cerebral functioning have been observed (cognitive functions, motor regulation, behavioral Only a few methods for piribedil in human plasma were published (Fanelli and Frigerio 1974;Sarati et al 1991;Sarati and Caccia 1992;Altiokka et al 2008;Uppuluri et al 2018;Sultana et al 2020). Fanelli and Frigerio (Fanelli and Frigerio 1974) described a method to detect piribedil in brain tissue and plasma by gas chromatography with flame ionization detector and liquid chromatography with mass fragmentographic technique.…”

“…mL -1 . Piribedil was also extracted from rat plasma and brain samples using HPLC with fluorescence and photodiode array detectors (Uppuluri et al 2018). In fluorescence and photodiode array detectors, linear response was achieved in the concentration ranges of (15-900), (450-9000) ng.…”

Figure 1 from: Alshishani A, Hasan I, Ghanayem F, Al-khasawneh S, Abu Dayah A (2022) Simple and rapid LC-MS/MS method for determination of Piribedil in human plasma. Pharmacia 69(3): 615-620. https://doi.org/10.3897/pharmacia.63.e86447

“…A non-ergot dopamine receptor agonist with interesting pharmacology in that it is a Ɒ2 and Ɒ3 receptor agonist in addition to a 2-adrenoceptor agonist (Regnier et al 1968;Gobert et al 2003). Several effects on dopamine-related cerebral functioning have been observed (cognitive functions, motor regulation, behavioral Only a few methods for piribedil in human plasma were published (Fanelli and Frigerio 1974;Sarati et al 1991;Sarati and Caccia 1992;Altiokka et al 2008;Uppuluri et al 2018;Sultana et al 2020). Fanelli and Frigerio (Fanelli and Frigerio 1974) described a method to detect piribedil in brain tissue and plasma by gas chromatography with flame ionization detector and liquid chromatography with mass fragmentographic technique.…”

Implementation of Quality by Design Methodology in Development and Validation of a New Stability-Indicating, Reverse Phase High-Performance Liquid Chromatography Method for the Rapid Estimation of Piribedil in Piribedil Prolonged Release Tablets

“…Ibrahim et al published potentiometric methodologies for quantifying piribedil in pharmaceutical preparations and urine [7,8] . The liquid chromatography methods for estimating piribedil and its metabolite in plasma were also reported [9,10] . An analytical method for estimating piribedil in pharmaceutical formulations by micellar electrokinetic capillary chromatography was also published [11] .…”