DEVELOPMENT AND VALIDATION OF RAPID REVERSE PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR SIMULTANEOUS ESTIMATION OF STIGMASTEROL  AND Β-Sitosterol IN EXTRACTS OF VARIOUS PARTS (LEAVES, STEMS, AND ROOTS) OF XANTHIUM STRUMARIUM LINN.

Kamboj, Anjoo; Bhatti, Manisha; Atri, Pooja

doi:10.22159/ajpcr.2017.v10i1.15021

Cited by 3 publications

(1 citation statement)

References 5 publications

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“…Gradient pattern way of two combined methods was rejected due to big changes in backpressure during analysis, very long chromatographic run time, and other different chromatographic conditions, required for acceptable separation of different triterpenoids. An isocratic elution requires only one pump and minimizes the variation of baseline and ghost peaks [37]. Therefore, two simple isocratic mode methods for different triterpenoids were further optimized.…”

Section: Optimization Of Methodsmentioning

confidence: 99%

Optimization, Validation and Application of HPLC-PDA Methods for Quantification of Triterpenoids in Vaccinium vitis-idaea L.

Vilkickytė

Raudonė

2021

Molecules

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Triterpenoids have regained much attention as promising multi-targeting bioactive agents of natural origin in the treatment of numerous disorders. Due to the high potential for phytopharmaceutical development, accurate qualitative and quantitative analysis of triterpenoids for screening and quality control is required. Vaccinium vitis-idaea L. (lingonberry) raw materials have aroused interest as a rich source of triterpenoids. However, currently, no validated, rapid, and easy-to-perform quantification method is available for the routine control of these compounds in lingonberries. This research aimed at developing and validating HPLC-PDA methods for the determination and screening of triterpenoids in extracts of lingonberry leaves, fruits, and flowers. The developed methods were deemed satisfactory by validation, which revealed acceptable analytical specificity, linearity (r2 > 0.9999), precision (RSD < 2%), trueness (94.70–105.81%), and sensitivity (LOD: 0.08–0.65 µg/mL). The real sample analysis demonstrated established methods applicability for quantification of 13 triterpenoids in lingonberries and emphasized differences between raw materials. Lingonberry fruits were distinguished by the richness of ursolic acid; lingonberry flowers by similar profile to fruits, but low content of neutral triterpenoids; whereas lingonberry leaves by the particularly high level of α-amyrin. Thus, the proposed methods proved to be reliable and applicable for quantification and routine analysis of triterpenoids in lingonberry samples.

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Section: Optimization Of Methodsmentioning

confidence: 99%

Optimization, Validation and Application of HPLC-PDA Methods for Quantification of Triterpenoids in Vaccinium vitis-idaea L.

Vilkickytė

Raudonė

2021

Molecules

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Improvement and prediction of the extraction parameters of lupeol and stigmasterol metabolites of Melia azedarach with response surface methodology

Rabbani,

Garoosi,

Haddad

et al. 2024

BMC Biotechnol

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Background Melia azedarach is known as a medicinal plant that has wide biological activities such as analgesic, antibacterial, and antifungal effects and is used to treat a wide range of diseases such as diarrhea, malaria, and various skin diseases. However, optimizing the extraction of valuable secondary metabolites of M. azedarach using alternative extraction methods has not been investigated. This research aims to develop an effective, fast, and environmentally friendly extraction method using Ultrasound-assisted extraction, methanol and temperature to optimize the extraction of two secondary metabolites, lupeol and stigmasterol, from young roots of M. azedarach using the response surface methodology. Methods Box-behnken design was applied to optimize different factors (solvent, temperature, and ultrasonication time). The amounts of lupeol and stigmasterol in the root of M. azedarach were detected by the HPLC-DAD. The required time for the analysis of each sample by the HPLC-DAD system was considered to be 8 min. Results The results indicated that the highest amount of lupeol (7.82 mg/g DW) and stigmasterol (6.76 mg/g DW) was obtained using 50% methanol at 45 °C and ultrasonication for 30 min, and 50% methanol in 35 °C, and ultrasonication for 30 min, respectively. Using the response surface methodology, the predicted conditions for lupeol and stigmasterol from root of M. azedarach were as follows; lupeol: 100% methanol, temperature 45 °C and ultrasonication time 40 min (14.540 mg/g DW) and stigmasterol 43.75% methanol, temperature 34.4 °C and ultrasonication time 25.3 min (5.832 mg/g DW). Conclusions The results showed that the amount of secondary metabolites lupeol and stigmasterol in the root of M. azedarach could be improved by optimizing the extraction process utilizing response surface methodology.

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Rapid Isolation of the Bioactive Metabolite of Cymbopogon Proximus and Development of Lc Method

El-Houssini

Metwally

2017

Int J Pharm Pharm Sci

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Objective: Development of a rapid isolation method to afford the bioactive metabolite (proximadiol: prox) from its herb (Cymbopogan proximus Stapf.) in the purest form and validate an LC method for its determination was our goal in this work. Methods:Prox was isolated by chromatographic techniques from the dichloromethane extract of the herb on alumina column, using petroleum ether-ethyl acetate solvent mixtures with increasing polarity.The determination of prox was achieved by LC method. The chromatographic separation was carried out on Thermo Hypersil ODS (4.5 x 250 mm, 10 µm) column, in the presence of 8-chlorotheophylline as an internal standard. The mobile phase was composed of 0.1 M phosphate buffer (pH: 3.5): methanol (60:40 v/v) and was pumped at a flow rate of 1 ml/min. The detection and quantification were done at 210 nm. Results:The structure elucidation of the isolated compound was identified on the basis of its spectral data.A simple and reproducible LC method was developed for the determination of the isolated purified Prox. Adequate separation and good resolution were obtained between prox and 8-chlorotheophylline. Quantification was achieved at 210 nm over concentration range 12.00-33.60 µg/ml with mean percentage recovery of 99.29±0.340. The method was validated in accordance with USP specifications. All analytical criterions were within acceptable range. The validity of the results was assessed by applying standard addition technique. The results obtained were compared with the reported method. Conclusion:The proposed method could be applied for routine quality control analysis of pharmaceutical formulations containing prox.

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DEVELOPMENT AND VALIDATION OF RAPID REVERSE PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR SIMULTANEOUS ESTIMATION OF STIGMASTEROL AND Β-Sitosterol IN EXTRACTS OF VARIOUS PARTS (LEAVES, STEMS, AND ROOTS) OF XANTHIUM STRUMARIUM LINN.

Cited by 3 publications

References 5 publications

Optimization, Validation and Application of HPLC-PDA Methods for Quantification of Triterpenoids in Vaccinium vitis-idaea L.

Optimization, Validation and Application of HPLC-PDA Methods for Quantification of Triterpenoids in Vaccinium vitis-idaea L.

Improvement and prediction of the extraction parameters of lupeol and stigmasterol metabolites of Melia azedarach with response surface methodology

Rapid Isolation of the Bioactive Metabolite of Cymbopogon Proximus and Development of Lc Method

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