2014
DOI: 10.5937/arhfarm1402069b
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Development and validation of RP-HPLC assay of chlorhexidine in gingival crevicular fluid

Abstract: SummaryA reversed -phase HPLC method with UV detection for determination of chlorhexidine in gingival crevicular fluid (GCF) was optimized and validated, using chlorpheniramine as an internal standard. The chromatographic separation was performed on Discovery C18 HPLC column with 0.01 mol L -1 phosphate buffer (pH=3.0), triethylamine and acetonitrile (66:1:33, V/V/V), as mobile phase. Under the optimized HPLC conditions, linearity was obtained in the range of 0.5-5.0 µg mL -1 with LOD 0.07 µg mL -1 and LLOQ 0.… Show more

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Cited by 5 publications
(5 citation statements)
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“…In order to quantify CHX concentrations in GCF samples after gel application we developed, optimized and validated a RP - HPLC method with UV detection for determination of CHX in GCF. The results from the validation have been published in our previous paper (Bogdanovska et al, 2014). The concentrations of CHX in GCF samples after the local administration are presented in Table 4 and a representative chromatogram of a patient’s GCF sample after the local treatment is shown in Fig.…”
Section: Resultsmentioning
confidence: 99%
“…In order to quantify CHX concentrations in GCF samples after gel application we developed, optimized and validated a RP - HPLC method with UV detection for determination of CHX in GCF. The results from the validation have been published in our previous paper (Bogdanovska et al, 2014). The concentrations of CHX in GCF samples after the local administration are presented in Table 4 and a representative chromatogram of a patient’s GCF sample after the local treatment is shown in Fig.…”
Section: Resultsmentioning
confidence: 99%
“…In modern analytical laboratories, there is a need for significant stability in analysis techniques, and therefore, analytical methods are continuously changing and developing. Over the past dozen years, numerous papers describing these methods have appeared, such as reversed-phase high-performance liquid chromatography [10][11][12], liquid chromatography coupled to tandem mass Summing up, through its activity against both Gram-positive and Gram-negative microorganisms and relatively low toxicity, chlorhexidine has a wide range of applications in human and veterinary medicine and dentistry. A large variety of products makes quality control, pharmacodynamic analytical tests, and pharmacokinetic studies necessary and still valid.…”
Section: Introductionmentioning
confidence: 99%
“…In modern analytical laboratories, there is a need for significant stability in analysis techniques, and therefore, analytical methods are continuously changing and developing. Over the past dozen years, numerous papers describing these methods have appeared, such as reversed-phase high-performance liquid chromatography [10][11][12], liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) [13][14][15], spectrophotometric methods [16,17], and electrochemical methods [18][19][20]. The Polish Pharmacopoeia [21] recommends titration in anhydrous acetic acid medium with perchloric acid at 0.1 mol L −1 .…”
Section: Introductionmentioning
confidence: 99%
“…In this case, UV-spectrophotometry serves as a reference method in the domestic practice of quality control of given drug [11]. At the same time, foreign sources of scientific information contain data on the possibility of analyzing chlorhexidine in various drugs by acid-base titration in protogenic solvent medium, as well as using HPLC and capillary electrophoresis [10,[12][13][14]. Among these methods, capillary electrophoresis is the most promising in controlling the quality of drugs containing chlorhexidine, and its advantages are well known and described in numerous scientific works [15][16][17][18].…”
Section: Introductionmentioning
confidence: 99%