Development and validation of simultaneous identification of 26 mammalian and poultry species by a multiplex assay

Mori, Chikahiro; Matsumura, Shuichi

doi:10.1007/s00414-021-02711-y

Cited by 10 publications

(4 citation statements)

References 52 publications

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“…New sequencing methods have been developed to assist species identification [ 616 ]. A multiplex PCR assay was developed to simultaneously identify 22 mammalian species (alpaca, Asiatic black bear, Bactrian camel, brown rat, cat, cow, common raccoon, dog, European rabbit, goat, horse, house mouse, human, Japanese badger, Japanese wild boar, masked palm civet, pig, raccoon dog, red fox, sheep, Siberian weasel, and sika deer) and four poultry species (chicken, domestic turkey, Japanese quail, and mallard) [ 617 ]. A number of other species identification assays have also been reported [ [618] , [619] , [620] ].…”

Section: Emerging Technologies Research Studies and Other Topicsmentioning

confidence: 99%

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

Butler

2023

Forensic Science International: Synergy

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Section: Emerging Technologies Research Studies and Other Topicsmentioning

confidence: 99%

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

Butler

2023

Forensic Science International: Synergy

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“…In animals, the most used markers are the mtDNA cytochrome b and cytochrome oxidase subunit 1 (COI) since the mutation rate of the latter coincides strongly with the rate of evolution of the species [111]. When only scant biological material is available, the use of mtDNA increases the success of the analysis [13].…”

Section: Species Identificationmentioning

confidence: 99%

“…To reduce the ambiguity of collaborations between laboratories skilled in animal genetics and law enforcement agencies within a forensic investigation, in 2008, during the International Society for Animal Genetics (ISAG; the Animal Forensic Genetics Standing Committee) conference, members of the committee defined animal forensic genetics as "the application of genetic techniques and theories relevant to legal or law enforcement issues concerning animal biological material". Recently, different genetic tools have been developed to improve the routine forensic procedures that need the identification of mammalian species [13]. In animal or human criminal investigations, the forensic genomics analysis of animal biological materials considers both domestic and wildlife [11].…”

Section: Introductionmentioning

confidence: 99%

The Revolution of Animal Genomics in Forensic Sciences

Cardinali

Tancredi

Lancioni

2023

IJMS

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Nowadays, the coexistence between humans and domestic animals (especially dogs and cats) has become a common scenario of daily life. Consequently, during a forensic investigation in civil or criminal cases, the biological material from a domestic animal could be considered “evidence” by law enforcement agencies. Animal genomics offers an important contribution in attacks and episodes of property destruction or in a crime scene where the non-human biological material is linked to the victim or perpetrator. However, only a few animal genetics laboratories in the world are able to carry out a valid forensic analysis, adhering to standards and guidelines that ensure the admissibility of data before a court of law. Today, forensic sciences focus on animal genetics considering all domestic species through the analysis of STRs (short tandem repeats) and autosomal and mitochondrial DNA SNPs (single nucleotide polymorphisms). However, the application of these molecular markers to wildlife seems to have gradually gained a strong relevance, aiming to tackle illegal traffic, avoid the loss of biodiversity, and protect endangered species. The development of third-generation sequencing technologies has glimmered new possibilities by bringing “the laboratory into the field”, with a reduction of both the enormous cost management of samples and the degradation of the biological material.

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“…Species barcoding can also be achieved using the RAPD approach [21], AFLPs [22], species-specific PCR [23], the SNaPshot assay [24], MRMA analysis [25], or qPCR assays [26]. Another type of barcoding methodology utilizes interspecies insertions/deletions, where the target region is a hypervariable mtDNA D-loop [27], the highly variable regions 12S rRNA 16S rRNA [28], and the control region (CR-mtDNA) length polymorphisms [29].…”

Section: Introductionmentioning

confidence: 99%

Capillary-Electrophoresis-Based Species Barcoding of Big Cats: CR-mtDNA-Length Polymorphism

Vankova,

Vanek

2024

Life

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This study aimed to provide an overview of the methodological approach used for the species determination of big cats. The molecular system described herein employs mitochondrial DNA control region (CR-mtDNA)-length polymorphism in combination with highly sensitive and precise capillary electrophoresis. We demonstrated that the described CR-mtDNA barcoding system can be utilized for species determination where the presence of biological material from big cats is expected or used as a confirmatory test alongside Sanger or massive parallel sequencing (MPS). We have also addressed the fact that species barcoding, when based on the analysis of mtDNA targets, can be biased by nuclear inserts of the mitochondrial genome (NUMTs). The CR-mtDNA barcoding system is suitable even for problematic and challenging samples, such as hair. CR-mtDNA-length polymorphisms can also distinguish hybrids from pure breeds.

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Development and validation of simultaneous identification of 26 mammalian and poultry species by a multiplex assay

Cited by 10 publications

References 52 publications

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

The Revolution of Animal Genomics in Forensic Sciences

Capillary-Electrophoresis-Based Species Barcoding of Big Cats: CR-mtDNA-Length Polymorphism

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