Development and Validation of Spectrophotometric Methods for Quantitative Estimation of Oxfendazole in Presence of Its Alkali-induced Degradation Product: A Comparative Study

Abstract

Objectives: This study aimed to develop and validate three simple, accurate, selective, reproducible and sensitive spectrophotometric methods for the determination of oxfendazole in the presence of its alkali-induced degradation product without preliminary separation. Methods: (A) first derivative spectrophotometry ( 1 D) method at 298.9 nm, (B) first derivative of ratio spectra ( 1 DD) method at 295.4 nm and (C) area under the curve (AUC) method at wavelength ranges of (290-294 nm) and (297-301 nm). All methods were applied in the range of (1-10µg mL -1 ). Results: These methods were validated and successfully applied for determination of oxfendazole in Unifendazole® suspension. The obtained results were statistically compared with those of the reported method by applying t-test and F-test at 95% confidence level and no significant difference was observed regarding accuracy and precision. Conclusion: The proposed methods are simple, rapid, economic, accurate and precise to determine oxfendazole in the presence of its alkali-induced degradation product without previous separation steps.