Development and validation of ultra-high-performance liquid chromatography for the determination of sennoside A and sennoside B in laxatives based on optimal chromatographic parameters

Tan, Peng; Yan, Zhao; Cao, Junling; Xiao, Xiaohe; Wang, Jiabo

doi:10.1039/c5ay02458g

Cited by 5 publications

(8 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…1 ), which ruled out the presence of sennoside B and other chemical constituent in our extracted sennoside from Cassia angustifolia . Our values of λ max are in good agreement with the λ max of standard sennoside A reported in the literature ( Tan, et al, 2015 ). The sennoside A concentration was calculated by the reported method with molar extinction coefficient, ε = 9430 L/mol/cm ( Hietala, et al, 1988 ).…”

Section: Resultssupporting

confidence: 91%

“…not given). Thus we may state confidently that our extracted dark yellow solid has sennoside A in the greatest concentration ( Sun and Su, 2002 , Tan et al, 2015 ).…”

Section: Resultsmentioning

confidence: 77%

“…260 nm and 440 nm and sennosides A and B exhibited absorption peak at 270 nm and 370 nm in ethylacetate ( Habib and Elsebakhy, 1980 ). Tan et al reported that the standard solution of sennoside A and sennoside B showed absorption peaks at 269 nm, 340 nm and 268 nm and 358 nm, respectively, in water-acetonitrile system ( Tan et al, 2015 ). They also suggested that the separation of both isomers from each other were also difficult.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Sennoside A drug capped biogenic fabrication of silver nanoparticles and their antibacterial and antifungal activities

Al-Ghamdi

Zaheer

Aazam

2020

Saudi Pharmaceutical Journal

View full text Add to dashboard Cite

Sennoside A (dianthrone glycoside) shows laxative properties and used as a folk traditional medicine. Sennoside A capped silver nanoparticles (Ag/sennoside A) were synthesized at room temperature for the first time by using sennoside A as reducing and capping agent. UV–visible spectroscopic data reveals that the absorption peaks of pure sennoside A was appeared at 266, and 340 nm, which red shifted to 304, and 354 nm at higher sennoside A concentration. Upon addition of the Ag + ions, an additional peak also observed at 398 nm, indicating the formation of spherical sennoside A capped silver nanoparticles (Ag/sennoside A). Cetyltrimethylammonium bromide (CTAB) was used a stabilizing agent to determine the role of cationic micelles on the nucleation and growth processes of Ag/sennoside A NPs formation. The 2,2-diphenyl-1-picrylhydrazyl nitrogen radical (DPPH · ), two bacteria strains (Staphylococcus aureus and Escherichia coli) and two yeast strains (Candida albicans ATCC 10231 and Candida parapsilosis ATCC 22019) were used to determine the antioxidant and antimicrobial properties of Ag/sennoside A NPs. In addition, Rhein-9-anthrone (4,5-dihydroxy-10-oxo-9 H -anthracene-2-carboxylate) was isolated from the acidic hydrolysis of glycoside linkage of sennoside A and characterized. The antioxidant and antimicrobial activities of rhein-9-anthrone were also determined against DPPH radical, antibacterial and antifungal strains. The minimum inhibitory concentration was determined and discussed.

show abstract

Section: Resultssupporting

confidence: 91%

“…not given). Thus we may state confidently that our extracted dark yellow solid has sennoside A in the greatest concentration ( Sun and Su, 2002 , Tan et al, 2015 ).…”

Section: Resultsmentioning

confidence: 77%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Sennoside A drug capped biogenic fabrication of silver nanoparticles and their antibacterial and antifungal activities

Al-Ghamdi

Zaheer

Aazam

2020

Saudi Pharmaceutical Journal

View full text Add to dashboard Cite

show abstract

“…Качественное определение антраценпроизводных в сенны листьях. В Государственной фармакопее Республики Беларусь [14] Ряд авторов также применяют метод высокоэффективной жидкостной хроматогрфии в анализе сенны листьев (таблица 3) [20,21,24,26,30,31,37,41,42,43,45,49]. Например, Peng T. и соавт.…”

Section: методы определения антраценпроизводных в сенны листьяхunclassified

Senna leaves: component composition, pharmacological properties, standardization

Romanyuk¹,

Moiseev²

2021

Humans and their Health

View full text Add to dashboard Cite

Objective: generalization and analysis of scientific data on the component composition, pharmacological properties and standardization of senna leaves. Materials and methods. The article provides an overview of scientific publications in the journals of the search platforms Scopus and PubMed, as well as the databases of the Russian Science Citation Index. The analysis of the range of herbal medicinal products was carried out according to the data of the State Registers of Medicines of the Republic of Belarus, the Russian Federation and the Republic of Kazakhstan using content analysis, comparison and generalization of data. Results. In addition to the laxative effect, determined by the presence of anthracene derivatives in senna leaves, the complex of their biologically active substances has antimicrobial, hypoglycemic, antioxidant, antifungal and antitumor effects. Also, the chemical composition of the plant is represented by fatty acids, triglycerides, flavonoids, macro- and microelements. The analysis of medicinal herbal preparations based on senna leaves registered in the Russian Federation, the Republic of Belarus and the Republic of Kazakhstan has been carried out. The differences in the methods of their standardization, given in the normative documentation on quality, have been revealed. The scientific experience of using the method of high performance liquid chromatography for the determination of anthracene derivatives in senna leaves is generalized. Conclusion. The complex of biologically active substances of senna leaves exhibits various pharmacological effects, which, possibly, will expand the range of combined preparations based on them. The use of a modern and selective method of high-performance liquid chromatography in the analysis of senna leaves is a very promising direction in the field of standardization of this type of medicinal plant material.

show abstract

“…We intend to opt for a faster chromatographic technique UPLC (Ultra high Performance Liquid Chromatography), for the said study and analytical validation 14 of developed method. An attempt has been made to determine whether UPLC can reduce analysis times without compromising the resolution and sensitivity.…”

Section: Fig 1: Chemical Structure Of Brivaracetammentioning

confidence: 99%

Untitled

2018

IJPSR

View full text Add to dashboard Cite

A novel stability-indicating mass compatible gradient Reverse Phase Ultra-Performance Liquid Chromatographic (RP-UPLC) method was developed for the quantitative determination of purity of brivaracetam drug substance samples in the presence of its impurities and degradation products. The method was developed using waters acquity UPLC BEH SHIELD RP18 (100 mm x 2.1 mm, 1.7 µm) column with mobile phase containing a gradient mixture of solvents A and B. The eluted compounds were monitored at 230 nm, the run time was 10 min within which brivaracetam and its four impurities were well separated. Brivaracetam was subjected to the stress conditions of oxidative, acid, base, hydrolytic, thermal and photolytic degradation. Brivaracetam was found to degrade significantly in acidic and slightly in oxidative stress conditions and stable in base, hydrolytic, and photolytic degradation conditions. The degradation products were well resolved from main peak and its impurities, proving the stability-indicating power of the method. The developed method was validated as per ICH guidelines with respect to specificity, linearity, limit of detection, limit of quantification, accuracy, precision and robustness.

show abstract

Development and validation of ultra-high-performance liquid chromatography for the determination of sennoside A and sennoside B in laxatives based on optimal chromatographic parameters

Abstract: A simple and sensitive UHPLC method was developed for simultaneous quantification of sennoside A and sennoside B in laxatives.

Cited by 5 publications

References 16 publications

Sennoside A drug capped biogenic fabrication of silver nanoparticles and their antibacterial and antifungal activities

Sennoside A drug capped biogenic fabrication of silver nanoparticles and their antibacterial and antifungal activities

Senna leaves: component composition, pharmacological properties, standardization

Untitled

Contact Info

Product

Resources

About