Development and validation of ultra-performance liquid chromatographic method with tandem mass spectrometry for determination of lenalidomide in rabbit and human plasma

Abstract

BackgroundLenalidomide (LND) is a potent novel thalidomide analog which demonstrated remarkable clinical activity in treatment of multiple myeloma disease via a multiple-pathways mechanism. Validated sensitive method with high throughput is required for the determination of lenalidomide for pharmacokinetics and toxicokinetic studies. Ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) is a preeminent analytical tool for rapid biomedical analysis.ResultsA simple, highly sensitive UPLC-MS/MS method was developed and validated for the determination of LND in rabbit and human plasma. After a simple protein precipitation using methanol, LND and carbamazepine (IS) were separated on Acquity UPLC BEH™ C18 column (50 × 2.1 mm, i.d. 1.7 μm, Waters, USA) using a mobile phase consisted of acetonitrile:water:formic acid (65:35:0.1%, v/v/v) pumped at a flow rate of 0.2 mL/min. LND and IS were eluted at 0.71 and 1.92 min, respectively. The mass spectrometric determination was carried out using an electrospray interface operated in the positive mode with multiple reaction monitoring (MRM) mode. The precursor to product ion transitions of m/z 260.1 > 149.0 and m/z 237.0 > 179.0 were used to quantify LND and IS, respectively. The method was linear in the concentration range of 0.23–1000 ng/mL with a limit of quantitation of 0.23 ng/mL. All the validation parameters were in the ranges acceptable by the guidelines of analytical method validation.ConclusionThe proposed UPLC-MS/MS method is simple, rapid and highly sensitive, and hence it could be reliable for pharmacokinetic and toxicokinetic study in both animals and humans.

Palm Kernel Shell as an effective adsorbent for the treatment of heavy metal contaminated water

“…Table 2 represents the correlation coefficient, R 2 value for each model and the value of rate constant, K 2 for the pseudo-second order. The kinetics of any reaction which follows the Pseudo second order reaction indicates that the adsorption has occurred via chemisorption 30,31 . As discussed in earlier, adsorption involves the chelation, adsorbents functional groups interactions with metal ions.…”

Ultra-high performance liquid chromatography-MS/MS (UHPLC-MS/MS) in practice: analysis of drugs and pharmaceutical formulations

“…A variety of sample preparation techniques have been applied in a bioanalytical method such as protein precipitation, liquid-liquid extraction, and solid-phase extraction [32]. Among these, the most widely used are protein precipitation and liquid-liquid extraction followed by solid-phase extraction which are used in different category such as antibiotic [33][34][35][36][37][38][39][40], anticancer [36,[41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56][57][58][59][60][61][62], antiviral [63][64][65][66][67], antifungal [68][69][70][71][72], cardiovascular [73][74][75][76][77]…”

Serum Pharmacochemistry Analysis Using UPLC-Q-TOF/MS after Oral Administration to Rats of Shenfu Decoction

“…Researchers have reported quantitative analysis of aconitum alkaloids of SFD in vitro as well as the pharmacokinetic behavior of Fuzi and drug-drug interaction mechanisms in herb pair decoctions [ 17 – 19 ]. Relatively few studies have been conducted on the material basis of SFD in vivo , and most researchers focused on Shenfu injection which is a kind of processed Shenfu formulation [ 20 , 21 ].…”