Development and Validation of UV Spectrophotometric Method for Simultaneous Estimation of Metformin and Glipizide in Tablet Dosage Form

Abstract

ABSTRACT:A simple, rapid and precise spectrophotometric method has been developed for simultaneous estimation of Metformin and Glipizide. The method involved estimation of Metformin and Glipizide by simultaneous equation at 272nm and 232nm respectively in their solution in water. This method was validated with respect to linearity, accuracy, precision, LOD and LOQ. Beer's law obeyed in the concentration range of 5-25µg/ml and 20-50µg/ml for Metformin and Glipizide respectively with the correlation coefficient of above 0.99. Limit of detection and quantification values were determined to be 0.214μg/ml and 0.649μg/ml for Metformin and 0.608μg/ml and 1.854μg/ml for Glipizide respectively. Mean recovery of Metformin and Glipizide were found to be in the range of 98-102% signifies the accuracy of the method. The method was found to be precise as %RSD was less than 2.

Stability‐indicating method development and validation for quantitative estimation of organic impurities of the antidiabetic drug glipizide in drug substance and pharmaceutical dosage form using HPLC

“…Glipizide is sold under the brand name Glucotrol. It is an oral hypoglycemic agent (Vaishali et al, 2013) and comes under the drug class of sulfonylureas, which are second‐generation drugs (Sireesha et al, 2016). These second‐generation drugs are very effective for the treatment of control the blood sugar levels in patients who have been suffer from type 2 diabetes mellitus (Lakshmi & Rajesh, 2011).…”

“…A literature survey revealed that several analytical methods are available for the estimation of glipizide in different combinations of drug products by UV, HPLC, UPLC, and UHPLC‐APCI‐TQ‐MS (ultra‐high‐performance liquid chromatography–atmospheric pressure chemical ionization with a triple quadrupole electrospray tandem mass spectrometry) techniques (Lakshmi & Rajesh, 2011; Rao & Vijayalakshmi, 2021; Sireesha et al, 2016; Sushama & Sameer, 2018; Swaroop et al, 2010). The available HPLC‐UV method (Sakshi & Gulshan., 2011) not covered the glipizide methyl carbamate impurity was mentioned in the United States Pharmacopeia (USP) monograph and observed the poor baseline and impurities are not separated clearly from the blank pattern.…”

Investigation of Chloride Anion Binding Properties of Glipizide Drug

“…GLP displays hypoglycemic (Pahwa et al, 2010), anticancer (Qi et al, 2014), and radical scavenging activities (by theoretical approach) (Hussan et al, 2022), and controlled release behaviors (Emami et al, 2014). Besides, a wide range of spectroscopic analyses of GLP has been performed in the literature (Ming et al, 2008;Adhikari et al, 2012;Prasad et al, 2013;Prakash & Iqbal, 2015;Ganesh et al, 2016;Jena et al, 2017;Ambadekar & Keni, 2018;Anwer et al, 2021).…”

Development, Computational Studies and Validation of Spectrophotometric Method of Metformin Hydrochloride in Pharmaceutical Formulations

“…24 Simple ultraviolet (UV) spectrophotometric methods were developed and validated for quantitation of metformin as single or in a binary mixture. [27][28][29][30][31][32][33][34][35][36] The proposed method presents a validated spectrophotometric method for the quantitation of metformin hydrochloride in bulk and pharmaceutical formulations. The method was based on the reaction between metformin and potassium permanganate at room temperature in basic medium.…”