Development and Validation of Uv-Visible Spectrophotometric Method for Analysis of Bosentan in Spiked Human Plasma

Abstract

Objective:The aim of the present study is to develop and validate a simple, efficient, economical and accurate UV-visible spectrophotometric method for estimation of bosentan in spiked human plasma. Methods:The analyte was extracted by Liquid-liquid Extraction (LLE) procedure using acetonitrile and chloroform. Absorbance of the analyte in the extract was measured at 270 nm using ethanol as a diluent. The developed method was validated for linearity, accuracy and robustness. Results:The proposed method was found to be linear in the range of 6 to 18 µg/ml. The correlation coefficient (r 2 Conclusion:The analytical technique presented here demonstrates shorter and easier sample preparation method, decreased analysis time and reduces the need for complicated or expensive equipment. The sample preparation method used in this study can also be further extended to higherend analytical techniques and other biological samples for quantification of bosentan.) was found to be 0.99. The results revealed that the linearity, accuracy and robustness of the developed method were within the acceptable range.

Simple Determination of Bosentan in Plasma Samples by Reversed-Phase High-Performance Liquid Chromatography

“…Bosentan's affinity for ETA receptors is somewhat greater than for ETB receptors 3 . The amount of Bosentan in biological samples has been estimated using methods such as gradient Reverse Phase-High Performance Liquid Chromatography (RP-HPLC), Solid Phase Extraction Liquid Chromatography-tandem Mass Spectrometry (SPE-LC-MS/ MS), and UV-visible spectrophotometric methods, which are costly and challenging to implement [4][5][6][7][8] .…”