Development, Identification and Validation of a Novel SSR Molecular Marker for Heat Resistance of Grapes Based on miRNA

Zhang, Lipeng; Song, Yue; Li, Junpeng; Liu, Jingjing; Zhang, Zhen; Xu, Yuanyuan; Fan, Dongying; Liu, Mingying; Ren, Yi; Xi, Xiaojun; Chen, Qiuju; He, Juan; Xu, Wenping; Song, Shiren; Liu, Huaifeng; Ma, Chao

doi:10.3390/horticulturae9080931

Cited by 4 publications

(1 citation statement)

References 60 publications

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“…This significantly affects the overall growth, development, and yield of grapevines. Biostimulants have been widely employed in crops due to their safety, non-polluting nature, low residue levels, and their ability to facilitate the growth and development of crops [29][30][31]. However, it remains uncertain whether they effectively work on grapevine seedlings under naturally high temperatures.…”

Section: Discussionmentioning

confidence: 99%

Effects of Different Biostimulants on Growth and Development of Grapevine Seedlings under High-Temperature Stress

Wu,

Zhong,

et al. 2024

Horticulturae

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High temperatures significantly affect the growth and development of grapevines, cause irreversible damage to plants, and severely impact grape production and quality. Biostimulants can promote the growth of plants and enhance their resistance to adverse stress. However, the effects of biostimulants on grapevines under high temperatures have not been studied in detail. To analyze the effects of various biostimulants on the growth and development of grape seedlings under high temperatures, we measured chlorophyll fluorescence parameters with observed seedling phenotypes under high temperatures in open field conditions in Turpan. We conducted a comprehensive analysis of the effects of different biostimulants on the growth, development, and photosynthesis of grapevine seedlings. Our study aimed to provide scientific evidence to improve cultivation methods for grapevines under high-temperature stress. The results revealed that biostimulants have a positive effect on promoting the growth of grapevine seedlings under high-temperature stress conditions. They also positively affect the accumulation of chlorophyll components in grapevine leaves, inhibiting chlorophyll degradation and maintaining photosynthesis. However, the effects of different biostimulants were inconsistent. A comprehensive analysis revealed the following effectiveness order: T2 > T1 > T3 > Control. These findings suggest that T2 is the most effective in alleviating high-temperature stress and promoting grapevine growth. We recommend the use of T2 to improve the cultivation of grapevine seedlings during high-temperature periods. This has implications for grape production in hot and arid climatic areas.

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Section: Discussionmentioning

confidence: 99%

Effects of Different Biostimulants on Growth and Development of Grapevine Seedlings under High-Temperature Stress

Wu,

Zhong,

et al. 2024

Horticulturae

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Assessment of heat tolerance and identification of miRNAs during high-temperature response in grapevine

Zhang,

Teng,

Song

et al. 2024

Front. Plant Sci.

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With global warming, heat stress has been recognized as a significant factor limiting grapevine development and fruit quality. MicroRNAs (miRNAs) are a class of small non-coding RNAs known to play crucial regulatory roles in stress resistance. Hence, there is an immediate requirement to cultivate and identify grapevine varieties that are resistant to heat and explore miRNA-mediated heat stress defense mechanisms. In this study, we assessed the thermal resistance of 38 grape germplasm resources and identified a series of miRNAs involved in heat stress resistance. The CK (25°C) and HS (45°C) groups of “Shenyue” cuttings of grapes were used as experimental materials for next-generation sequencing and construct libraries of small RNAs. A total of 177 known and 20 novel miRNAs were detected in the libraries. Differential expression analysis identified 65 differentially expressed miRNAs (DEMs) using the DE-Seq procedure. Furthermore, RT-qPCR validation confirmed complementary expression profiles of eight DEMs and their target genes between the HS and CK groups. Heterologous transformation further identified the function of Vvi-miR3633a downregulated under heat stress in Arabidopsis. In the heterologous expression lines, the survival rate was reduced by high temperature treatment indicating the ability of Vvi-miR3633a to regulate heat resistance. Assessing the heat resistance of grape species and the expression patterns of miRNA in response to high temperatures may reveal the molecular processes of heat resistance regulation mediated by miRNA in grapes under heat stress.

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Negative Regulatory Role of Non-Coding RNA Vvi-miR3633a in Grapevine Leaves and Callus under Heat Stress

Zhang,

Teng,

et al. 2024

Horticulturae

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The grapevine, a globally significant fruit and an essential fruit tree species in China, is vulnerable to the adverse effects of high temperatures. Understanding the roles of microRNA and transcription factors in plant development and stress resistance is crucial for mitigating the impact of high temperature on grape growth and yield. This study investigates the response of miRNA to high-temperature stress in grape leaves. The expression level of Vvi-miR3633a was found to be inhibited under heat treatment in both Thompson seedless and Shen yue varieties, while its potential target genes (Vv-Atg36 and Vv-GA3ox2) were induced. Through transgenic overexpression experiments, it was demonstrated that Vvi-miR3633a plays a role in thermal response by affecting the expression of target genes. Furthermore, under heat stress conditions, overexpression of Vvi-miR3633a in grape callus decreased heat resistance compared to the control group (CK). The study also revealed that the target genes of Vvi-miR3633a regulate the expression of oxidase synthesis genes VvSOD and VvCAT, leading to reduced oxidase synthesis which may compromise the oxidation system. Additionally, the expression level of heat shock proteins in the transgenic lines was changed compared to the control (CK). Overall, this research provides valuable insights into understanding the molecular mechanisms involved in different crossing/breeding programs to produce heat-resistant grape varieties. Such varieties can be appropriate to propagate in warm climate areas with high temperature conditions.

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Development, Identification and Validation of a Novel SSR Molecular Marker for Heat Resistance of Grapes Based on miRNA

Cited by 4 publications

References 60 publications

Effects of Different Biostimulants on Growth and Development of Grapevine Seedlings under High-Temperature Stress

Effects of Different Biostimulants on Growth and Development of Grapevine Seedlings under High-Temperature Stress

Assessment of heat tolerance and identification of miRNAs during high-temperature response in grapevine

Negative Regulatory Role of Non-Coding RNA Vvi-miR3633a in Grapevine Leaves and Callus under Heat Stress

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