Development of 10 polymorphic microsatellite markers from herbicide‐bleached tissues of the brooding pocilloporid coral <i>Seriatopora hystrix</i>

Underwood, Jim N.; Souter, Petra; Ballment, E.; Lutz, Adrian; Oppen, Madeleine J. H. van

doi:10.1111/j.1471-8286.2005.01183.x

Cited by 38 publications

(37 citation statements)

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“…Sh2-002, Sh2-006, Sh3-004, Sh3-007 and Sh4-001 were developed by Underwood et al (2006) and previously used in S. hystrix from Australia van Oppen et al 2008;Noreen et al 2009). Locus Sh4.24 was originally employed in S. hystrix from the Red Sea (Maier et al 2005).…”

Section: Molecular Analysesmentioning

confidence: 99%

“…In recent years, a number of species-specific microsatellites were developed (Maier et al 2001(Maier et al , 2005Underwood et al 2006) and successfully applied to investigate population genetic processes on different spatial scales (e.g., Maier et al 2005Maier et al , 2009Underwood et al 2007Underwood et al , 2009van Oppen et al 2008van Oppen et al , 2011Noreen et al 2009). Given that these and other surveys include individual-based analyses, the aims of our study were to (1) investigate whether genetically heterogeneous colonies occur in natural S. hystrix stands and (2) distinguish between the two underlying mechanisms, mosaicism and chimerism.…”

Section: Introductionmentioning

confidence: 99%

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Intracolonial genetic variation in the scleractinian coral Seriatopora hystrix

et al. 2011

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In recent years, increasing numbers of studies revealed intraorganismal genetic variation, primarily in modular organisms like plants or colonial marine invertebrates. Two underlying mechanisms are distinguished: Mosaicism is caused by somatic mutation, whereas chimerism originates from allogeneic fusion. We investigated the occurrence of intracolonial genetic variation at microsatellite loci in five natural populations of the scleractinian coral Seriatopora hystrix on the Great Barrier Reef. This coral is a widely distributed, brooding species that is at present a target of intensive population genetic research on reproduction and dispersal patterns. From each of 155 S. hystrix colonies, either two or three samples were genotyped at five or six loci. Twenty-seven (*17%) genetically heterogeneous colonies were found. Statistical analyses indicated the occurrence of both mosaicism and chimerism. In most cases, intracolonial variation was found only at a single allele. Our analyses suggest that somatic mutations present a major source of genetic heterogeneity within a single colony. Moreover, we observed large, apparently stable chimeric colonies that harbored clearly distinct genotypes and contrast these findings with the patterns typically observed in laboratory-based experiments. We discuss the error that mosaicism and chimerism introduce into population genetic analyses.

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Section: Molecular Analysesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Intracolonial genetic variation in the scleractinian coral Seriatopora hystrix

et al. 2011

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“…DNA was extracted following Wilson et al (2002). Ten loci were amplified using fluorescently labelled primers in three multiplex PCRs as described in Underwood et al (2006). Samples with rare (frequency , 0.05) private alleles were PCR amplified and genotyped at least twice to confirm the allele's authenticity.…”

Section: Methodsmentioning

confidence: 99%

Genetic diversity and connectivity in a brooding reef coral at the limit of its distribution

2009

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Remote populations are predicted to be vulnerable owing to their isolation from potential source reefs, and usually low population size and associated increased extinction risk. We investigated genetic diversity, population subdivision and connectivity in the brooding reef coral Seriatopora hystrix at the limits of its Eastern Australian (EA) distribution and three sites in the southern Great Barrier Reef (GBR). Over the approximately 1270 km survey range, high levels of population subdivision were detected (global F ST ¼ 0.224), with the greatest range in pairwise F ST values observed among the three southernmost locations: Lord Howe Island, Elizabeth Reef and Middleton Reef. Flinders Reef, located between the GBR and the more southerly offshore reefs, was highly isolated and showed the signature of a recent bottleneck. High pairwise F ST values and the presence of multiple genetic clusters indicate that EA subtropical coral populations have been historically isolated from each other and the GBR. One putative first-generation migrant was detected from the GBR into the EA subtropics. Occasional long-distance dispersal is supported by changes in species composition at these high-latitude reefs and the occurrence of new species records over the past three decades. While subtropical populations exhibited significantly lower allelic richness than their GBR counterparts, genetic diversity was still moderately high. Furthermore, subtropical populations were not inbred and had a considerable number of private alleles. The results suggest that these high-latitude S. hystrix populations are supplemented by infrequent longdistance migrants from the GBR and may have adequate population sizes to maintain viability and resist severe losses of genetic diversity.

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“…Most coral species produce eggs and larvae that lack Symbiodinium, making these early life stages amenable for inoculation with stress-tolerant Symbiodinium types to explore the phenotypic benefits of such novel symbioses. Additionally, larvae of maternally transmitting species can be experimentally bleached (82) and similarly exposed to novel Symbiodinium types. A major challenge with this approach will be to find a way for manipulated coral-Symbiodinium associations to remain stable (83,84).…”

Section: Assisted Evolution Approaches To Build Coral Reef Resiliencementioning

confidence: 99%

Building coral reef resilience through assisted evolution

Oppen

Oliver

Putnam

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

755

659

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The genetic enhancement of wild animals and plants for characteristics that benefit human populations has been practiced for thousands of years, resulting in impressive improvements in commercially valuable species. Despite these benefits, genetic manipulations are rarely considered for noncommercial purposes, such as conservation and restoration initiatives. Over the last century, humans have driven global climate change through industrialization and the release of increasing amounts of CO 2 , resulting in shifts in ocean temperature, ocean chemistry, and sea level, as well as increasing frequency of storms, all of which can profoundly impact marine ecosystems. Coral reefs are highly diverse ecosystems that have suffered massive declines in health and abundance as a result of these and other direct anthropogenic disturbances. There is great concern that the high rates, magnitudes, and complexity of environmental change are overwhelming the intrinsic capacity of corals to adapt and survive. Although it is important to address the root causes of changing climate, it is also prudent to explore the potential to augment the capacity of reef organisms to tolerate stress and to facilitate recovery after disturbances. Here, we review the risks and benefits of the improvement of natural and commercial stocks in noncoral reef systems and advocate a series of experiments to determine the feasibility of developing coral stocks with enhanced stress tolerance through the acceleration of naturally occurring processes, an approach known as (human)-assisted evolution, while at the same time initiating a public dialogue on the risks and benefits of this approach.adaptation | climate change | microbial symbionts | selective breeding | transgenerational acclimatization

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Development of 10 polymorphic microsatellite markers from herbicide‐bleached tissues of the brooding pocilloporid coral Seriatopora hystrix

Cited by 38 publications

References 9 publications

Intracolonial genetic variation in the scleractinian coral Seriatopora hystrix

Intracolonial genetic variation in the scleractinian coral Seriatopora hystrix

Genetic diversity and connectivity in a brooding reef coral at the limit of its distribution

Building coral reef resilience through assisted evolution

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