Development of 34 New Microsatellite Markers from Actinidia arguta: Intra- and Interspecies Genetic Analysis

Abstract

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT The present study investigated the isolation and characterization of 34 polymorphic microsatellite markers developed from Actinidia arguta (Sieb. and Zucc.) Planch. ex Miq. var arguta. These markers produced 349 alleles in eight Actinidia species, with an average of 10.3 alleles per locus. Observed heterozygosity ranged from 0.50 to 0.87 (mean = 0.72), and polymorphism information content ranged from 0.37 to 0.88 (mean = 0.69). The phylogenetic relationship obtained using microsatellite markers showed minor clustering and population differences among species while 38 A. arguta accessions fell into two subgroups. These newly developed polymorphic microsatellite markers will be very useful in sustainable genetic conservation, marker-assisted breeding, and classification of the Actinidia genus.

Novel Microsatellite Markers Acquired from Rubus coreanus Miq. and Cross-Amplification in Other Rubus Species

“…Microsatellite motifs were discovered in 546 clones. The enrichment efficiency of microsatellites (84.5%) was higher than for groundnut at 68% [ 17 ], Japanese apricot at 57.0% [ 18 ], lychee at 52.0% [ 19 ], and Actinidia arguta at 74.2% [ 20 ].…”