Development of a 16 X-STR multiplex PCR system for kinship analysis and its applicability for the Sinhalese population in Sri Lanka

X-chromosomal STR based genetic polymorphisms and demographic history of Sri Lankan ethnicities and their relationship with global populations

“…The cluster II, which had the highest presentation of LD among the four clusters, displayed a highly significant ( P = 0.0000) LD between the two marker pairs, DXS10074-XS10075 (in Sinhalese, Indian Tamils and Moors) and DXS7132-DXS10075 (among the Sinhalese). However, there was no LD detected between DXS10079 and DXS10074 among Sinhalese in contrast to the results obtained for our initial study, which was conducted with 120 Sinhalese males 14 . This disparity of observations might have caused by the limitations in the initial study with respect to the sample size requirements specified for LD analysis 49 .…”

“…Finger pricked blood samples were collected from 838 unrelated individuals from the four ethnic groups in the Sri Lankan population; 426 samples from Sinhalese (60.6% males), 154 samples from the Sri Lankan Tamils (50% males), 128 samples from Indian Tamils (49.2% males), and 130 samples from Sri Lankan Moors (51.5% males). Genomic DNA was extracted using Chelex-100 method 59 and subjected to PCR amplification using the single tube 16 X-STR multiplex system described in Perera et al 14 . Amplified products were resolved with capillary gel electrophoresis using ABI 3500 Genetic Analyzer (Thermo Fisher Scientific, USA) and data analysis allele designation was performed using GeneMapper IDX software (Thermo Fisher Scientific, USA).…”

“…Recognizing this vital need exist in the field of molecular genetics in Sri Lanka, we recently developed a multiplex X-STR system with 16 X-STR markers (Fig. 1 ) distributed from 9.198 to 149.460 Mb of the X chromosome 14 with the aim of incorporating X-STR analysis in to molecular forensic and evolutionary genetics investigations in the country. Thirteen of these STR markers are in four closely linked clusters (each spanning < 3 cM) that are likely to produce stable haplotypes (Cluster I; DXS10148-DXS10135-DXS8378 (Xp22), Cluster II; DXS7132-DXS10079-DXS10074-DXS10075 (Xq12), Cluster III: DXS6801-DXS6809-DXS6789 (Xq21), Cluster IV; DXS7424-DXS101-DXS7133 (Xq22)).…”

Genetic Polymorphisms and Forensic Efficiency of 16 X-chromosomal STR&nbsp;Loci for Sri Lankan Population

“…Finger pricked blood samples were collected from 838 unrelated individuals from the four ethnic groups in the Sri Lankan population; 426 samples from Sinhalese (60.6% males), 154 samples from the Sri Lankan Tamils (50% males), 128 samples from Indian Tamils (49.2% males), and 130 samples from Sri Lankan Moors (51.5% males). Genomic DNA was extracted using Chelex-100 method 57 and subjected to PCR ampli cation using the single tube 16 X-STR multiplex system described in Perera et al 14 . Ampli ed products were resolved with capillary gel electrophoresis using ABI 3500 Genetic Analyzer (Thermo Fisher Scienti c, USA) and data analysis allele designation was performed using GeneMapper IDX software (Thermo Fisher Scienti c, USA).…”

“…Recognizing this vital need exist in the eld of molecular genetics in Sri Lanka, we recently developed a multiplex X-STR system with 16 X-STR markers distributed from 9.198 Mb to 149.460 Mb of the X chromosome 14 with the aim of incorporating X-STR analysis in to molecular forensic and evolutionary genetics investigations in the country. Thirteen of these STR markers are in four closely linked clusters (each spanning < 3 cM) that are likely to produce stable haplotypes (Cluster I; DXS10148-DXS10135-DXS8378 (Xp22), Cluster II; DXS7132-DXS10079-DXS10074-DXS10075 (Xq12), Cluster III: DXS6801-DXS6809-DXS6789 (Xq21), Cluster IV; DXS7424-DXS101-DXS7133 (Xq22)).…”