Development of a 3D Tissue‐Engineered Skeletal Muscle and Bone Co‐culture System

Wragg, Nicholas M.; Mosqueira, Diogo; Blokpeol‐Ferreras, Lia; Capel, Andrew J.; Player, Darren J.; Martin, Neil R.W.; Liu, Yang; Lewis, Mark P.

doi:10.1002/biot.201900106

Cited by 4 publications

(3 citation statements)

References 90 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Myogenic cell lineages are typically cultured in a high-glucose basal medium with at least 10% FBS while the EGM-2 medium only contains 5% FBS. The negative impact on myogenic cell lineages when defining co-culture media has been described by us and others [10,12,40]. Still, the BAM-2s provided the best trade-off between vasculogenesis and myogenesis in a tissue engineered muscle construct.…”

Section: Discussionmentioning

confidence: 77%

Functional evaluation of prevascularization in one-stage versus two-stage tissue engineering approach of human bio-artificial muscle

et al. 2020

View full text Add to dashboard Cite

A common shortcoming of current tissue engineered constructs is the lack of a functional vasculature, limiting their size and functionality. Prevascularization is a possible strategy to introduce vascular networks in these constructs. It includes among others co-culturing target cells with endothelial (precursor) cells that are able to form endothelial networks through vasculogenesis. In this paper, we compared two different prevascularization approaches of bio-artificial skeletal muscle tissue (BAM) in vitro and in vivo. In a one-stage approach, human muscle cells were directly co-cultured with endothelial cells in 3D. In a two-stage approach, a one week old BAM containing differentiated myotubes was coated with a fibrin hydrogel containing endothelial cells. The obtained endothelial networks were longer and better interconnected with the two-stage approach. We evaluated whether prevascularization had a beneficial effect on in vivo perfusion of the BAM and improved myotube survival by implantation on the fascia of the latissimus dorsi muscle of NOD/SCID mice for 5 or 14 d. Also in vivo, the two-stage approach displayed the highest vascular density. At day 14, anastomosis of implanted endothelial networks with the host vasculature was apparent. BAMs without endothelial networks contained longer and thicker myotubes in vitro, but their morphology degraded in vivo. In contrast, maintenance of myotube morphology was well supported in the two-stage prevascularized BAMs. To conclude, a two-stage prevascularization approach for muscle engineering improved the vascular density in the construct and supported myotube maintenance in vivo.

show abstract

Section: Discussionmentioning

confidence: 77%

Functional evaluation of prevascularization in one-stage versus two-stage tissue engineering approach of human bio-artificial muscle

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Thereafter, 3D-cultures appeared attractive to work out as a model since they include cell-cell contacts and so, putative juxtacrine signals ( 292 , 306 , 307 ). As an example, 3D in vitro structures were proposed for muscle/bone co-cultures using established cell lines for muscle (murine C2C12) and bone (human TE85 ( 308 );). In optimal cases however, cultured cells look as closely as possible to those inside the tissues and keep this phenotype as long as possible to signal properly to the other cell types.…”

Section: New Approaches To Specific Analysis Of Tissue Interactionsmentioning

confidence: 99%

Recent advances in the crosstalk between adipose, muscle and bone tissues in fish

et al. 2023

View full text Add to dashboard Cite

Control of tissue metabolism and growth involves interactions between organs, tissues, and cell types, mediated by cytokines or direct communication through cellular exchanges. Indeed, over the past decades, many peptides produced by adipose tissue, skeletal muscle and bone named adipokines, myokines and osteokines respectively, have been identified in mammals playing key roles in organ/tissue development and function. Some of them are released into the circulation acting as classical hormones, but they can also act locally showing autocrine/paracrine effects. In recent years, some of these cytokines have been identified in fish models of biomedical or agronomic interest. In this review, we will present their state of the art focusing on local actions and inter-tissue effects. Adipokines reported in fish adipocytes include adiponectin and leptin among others. We will focus on their structure characteristics, gene expression, receptors, and effects, in the adipose tissue itself, mainly regulating cell differentiation and metabolism, but in muscle and bone as target tissues too. Moreover, lipid metabolites, named lipokines, can also act as signaling molecules regulating metabolic homeostasis. Regarding myokines, the best documented in fish are myostatin and the insulin-like growth factors. This review summarizes their characteristics at a molecular level, and describes both, autocrine effects and interactions with adipose tissue and bone. Nonetheless, our understanding of the functions and mechanisms of action of many of these cytokines is still largely incomplete in fish, especially concerning osteokines (i.e., osteocalcin), whose potential cross talking roles remain to be elucidated. Furthermore, by using selective breeding or genetic tools, the formation of a specific tissue can be altered, highlighting the consequences on other tissues, and allowing the identification of communication signals. The specific effects of identified cytokines validated through in vitro models or in vivo trials will be described. Moreover, future scientific fronts (i.e., exosomes) and tools (i.e., co-cultures, organoids) for a better understanding of inter-organ crosstalk in fish will also be presented. As a final consideration, further identification of molecules involved in inter-tissue communication will open new avenues of knowledge in the control of fish homeostasis, as well as possible strategies to be applied in aquaculture or biomedicine.

show abstract

“…Both techniques allow a precise control of the TME by applying a tuneable perfusion of media that mimics blood flow and facilitates a continuous access of nutrients, oxygen and drugs [54] , [55] , [56] . In addition, these systems can reproduce the complexity of tissue by adding layers or compartments by co-culture of different various sets of cells in the presence of various ECM components [57] , [58] , [59] .…”

Section: Introductionmentioning

confidence: 99%

In vitro three-dimensional cell cultures for bone sarcomas

Muñoz-García

Jubelin

Loussouarn

et al. 2021

Journal of Bone Oncology

View full text Add to dashboard Cite

Highlights 3D cell cultures present similar drugs resistance features than in vivo tumours. Unlike 2D cell cultures, 3D culture approaches mimic better the tumour microenvironment. Collagen scaffolds result in a better osteosarcoma 3D proliferation than soft hydrogels. Osteosarcoma 3D models constitute an appealing approach for bone mineralisation studies. Combination of microfluidic/bioprinting technology with bone 3D cultures as ideal tools to study bone sarcomas.

show abstract

Development of a 3D Tissue‐Engineered Skeletal Muscle and Bone Co‐culture System

Cited by 4 publications

References 90 publications

Functional evaluation of prevascularization in one-stage versus two-stage tissue engineering approach of human bio-artificial muscle

Functional evaluation of prevascularization in one-stage versus two-stage tissue engineering approach of human bio-artificial muscle

Recent advances in the crosstalk between adipose, muscle and bone tissues in fish

In vitro three-dimensional cell cultures for bone sarcomas

Contact Info

Product

Resources

About