Development of a bio-electrochemical immunosensor based on the immobilization of SPINNTKPHEAR peptide derived from HPV-L1 protein on a gold electrode surface

Valencia, Drochss P.; Dantas, Luiza Maria Ferreira; Lara, Alexandre; Garcı́a, Javier; Rivera, Zuly; Rosas, Juan M.; Bertotti, Mauro

doi:10.1016/j.jelechem.2016.03.040

Cited by 21 publications

(12 citation statements)

References 23 publications

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“…Based in our previous results [ 17 ], we designed two peptides: HPV-2 and HPV-3. Both of them contain: (i) an antigenic sequence (underlined); (ii) a cysteine residue, which was introduced since it is well-known that compounds containing thiol groups are strongly coordinated with a variety of metals, including gold, silver, platinum, and copper [ 28 , 29 ].…”

Section: Resultsmentioning

confidence: 99%

“…In this context, we previously designed a bioelectrochemical immunosensor; specifically, the synthetic peptide HPV-1: Fc–Ahx–SPINNTKPHEAR was physically immobilized on a gold electrode, and the coating was done through the drop-casting method, using the Nafion polymer. HPV-1 was designed containing a ferrocene probe, a spacer, and a sequence derived from L1-HPV protein [ 17 ]. The electrochemical behavior of the modified electrode was examined via cyclic voltammetry in order to evaluate the redox behavior of the ferrocene moiety.…”

Section: Introductionmentioning

confidence: 99%

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Design, Synthesis, and Use of Peptides Derived from Human Papillomavirus L1 Protein for the Modification of Gold Electrode Surfaces by Self-Assembled Monolayers

et al. 2017

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In order to obtain gold electrode surfaces modified with Human Papillomavirus L1 protein (HPV L1)-derived peptides, two sequences, SPINNTKPHEAR and YIK, were chosen. Both have been recognized by means of sera from patients infected with HPV. The molecules, Fc-Ahx-SPINNTKPHEAR, Ac–C–Ahx-(Fc)KSPINNTKPHEAR, Ac–C–Ahx-SPINNTKPHEAR(Fc)K, C–Ahx–SPINNTKPHEAR, and (YIK)2–Ahx–C, were designed, synthesized, and characterized. Our results suggest that peptides derived from the SPINNTKPHEAR sequence, containing ferrocene and cysteine residues, are not stable and not adequate for electrode surface modification. The surface of polycrystalline gold electrodes was modified with the peptides C-Ahx-SPINNTKPHEAR or (YIK)2-Ahx-C through self-assembly. The modified polycrystalline gold electrodes were characterized via infrared spectroscopy and electrochemical measurements. The thermodynamic parameters, surface coverage factor, and medium pH effect were determined for these surfaces. The results indicate that surface modification depends on the peptide sequence (length, amino acid composition, polyvalence, etc.). The influence of antipeptide antibodies on the voltammetric response of the modified electrode was evaluated by comparing results obtained with pre-immune and post-immune serum samples.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Design, Synthesis, and Use of Peptides Derived from Human Papillomavirus L1 Protein for the Modification of Gold Electrode Surfaces by Self-Assembled Monolayers

et al. 2017

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“…Especially the electrochemical immunosensors have emerged as an alternative diagnostic methods for the traditional clinical assays in medical diagnostics and pandemic diseases management ( Hussein et al, 2020 ; Ranjan et al, 2021 ; Torrente-Rodríguez et al, 2020 ). This section summarizes the sensor systems in which the recognition matrix is comprised of antibodies for the selective electrochemical detection of hepatitis A ( Mandli et al, 2017 ), of hepatitis B ( Akkapinyo et al, 2020 ; Wei et al, 2020 ), of hepatitis C ( Zhao and Liu, 2016 ), five types of hepatitis ( Tang et al, 2010 ), HPV ( Piro et al, 2011 ; Valencia et al, 2016 ; Zari et al, 2009 ), HIV ( Akkapinyo et al, 2020 ; Macchia et al, 2020 ), Zika ( Cabral-Miranda et al, 2018 ; Draz et al, 2018 ; Faria and Mazon, 2019 ; Kaushik et al, 2018 ), Dengue ( Cheng et al, 2012 ; Nawaz et al, 2018 ), Rotavirus ( Attar et al, 2016 ; Liu et al, 2013 ) and Influenza virus ( Zhou et al, 2013 ).…”

Section: Electrochemical Diagnosticsmentioning

confidence: 99%

“… S NO Virus Recognition Matrix Method Concentration Range LOD Ref 1 HAV Carbon nanopowder paste electrode/antibody labeled with peroxidase Chronoamperometry 2×10 -4 – 5×10 -3 IU. mL -1 26 ×10 -5 IU mL -1 ( Mandli et al, 2017 ) 2 HPV Au peptide (SPINNTKPHEAR) linked to a 6-aminohexanoic (Ahx) residue and ferrocene (Fc) Chronoamperometry 0.010–0.020 μg L −1 0.010 μg L −1 ( Valencia et al, 2016 ) 3 HPV GCE/anti HPV-16-L1:anti-HPV complex SWV - 0.1 nmol dm −3 ( Piro et al, 2011 ) 4 HPV SCPGE/DNA SWV 0–5 ng mL -1 0.3 ng mL -1 ( Zari et al, 2009 ) 5 HBV Graphene paste electrode|Au NPs/Nafion-cysteine/Antibody DPV 0.5–200 ng mL -1 0.01 ng mL -1 ( Huang et al, 2012 ) 6 HBV SPCE/Graphene Oxide/Fe 3 O 4 /Prussian Blue Nanocomposites/Antibody DPV 0.5 pg mL -1 - 200 ng mL -1 0.166 pg mL -1 ( Wei et al, 2020 ...…”

Section: Electrochemical Diagnosticsmentioning

confidence: 99%

Electrochemical diagnostics of infectious viral diseases: Trends and challenges

Goud

Reddy

Khorshed

et al. 2021

Biosensors and Bioelectronics

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Infectious diseases caused by viruses can elevate up to undesired pandemic conditions affecting the global population and normal life function. These in turn impact the established world economy, create jobless situations, physical, mental, emotional stress, and challenge the human survival. Therefore, timely detection, treatment, isolation and prevention of spreading the pandemic infectious diseases not beyond the originated town is critical to avoid global impairment of life (e.g., Corona virus disease - 2019, COVID-19). The objective of this review article is to emphasize the recent advancements in the electrochemical diagnostics of twelve life-threatening viruses namely - COVID-19, Middle east respiratory syndrome (MERS), Severe acute respiratory syndrome (SARS), Influenza, Hepatitis, Human immunodeficiency virus (HIV), Human papilloma virus (HPV), Zika virus, Herpes simplex virus, Chikungunya, Dengue, and Rotavirus. This review describes the design, principle, underlying rationale, receptor, and mechanistic aspects of sensor systems reported for such viruses. Electrochemical sensor systems which comprised either antibody or aptamers or direct/mediated electron transfer in the recognition matrix were explicitly segregated into separate sub-sections for critical comparison. This review emphasizes the current challenges involved in translating laboratory research to real-world device applications, future prospects and commercialization aspects of electrochemical diagnostic devices for virus detection. The background and overall progress provided in this review are expected to be insightful to the researchers in sensor field and facilitate the design and fabrication of electrochemical sensors for life-threatening viruses with broader applicability to any desired pathogens.

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“…The International Agency for Research on Cancer (IARC) estimates that the number of cases of invasive cervical cancer in 2020 was 557,088 with more than 297,122 deaths caused by the disease. On incidence rates, cervical cancer was the third most common female cancer in the world in 2018 [ 1 ] and, after breast cancer and colorectal cancer, is the third leading cause of death in women worldwide [ 2 ]. HPV infection is a well-established cause of cervical cancer, and there is growing evidence that could be relevant in other anogenital cancers such as anal, vulval, vaginal, and penile, in addition to head and neck cancers [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Resistive DNA Biosensor for the Detection of HPV Type 16

et al. 2021

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In this work, a low-cost and rapid electrochemical resistive DNA biosensor based on the current relaxation method is described. A DNA probe, complementary to the specific human papillomavirus type 16 (HPV-16) sequence, was immobilized onto a screen-printed gold electrode. DNA hybridization was detected by applying a potential step of 30 mV to the system, composed of an external capacitor and the modified electrode DNA/gold, for 750 µs and then relaxed back to the OCP, at which point the voltage and current discharging curves are registered for 25 ms. From the discharging curves, the potential and current relaxation were evaluated, and by using Ohm’s law, the charge transfer resistance through the DNA-modified electrode was calculated. The presence of a complementary sequence was detected by the change in resistance when the ssDNA is transformed in dsDNA due to the hybridization event. The target DNA concentration was detected in the range of 5 to 20 nM. The results showed a good fit to the regression equation ΔRtotal(Ω)=2.99 × [DNA]+81.55, and a detection limit of 2.39 nM was obtained. As the sensing approach uses a direct current, the electronic architecture of the biosensor is simple and allows for the separation of faradic and nonfaradaic contributions. The simple electrochemical resistive biosensor reported here is a good candidate for the point-of-care diagnosis of HPV at a low cost and in a short detection time.

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Development of a bio-electrochemical immunosensor based on the immobilization of SPINNTKPHEAR peptide derived from HPV-L1 protein on a gold electrode surface

Cited by 21 publications

References 23 publications

Design, Synthesis, and Use of Peptides Derived from Human Papillomavirus L1 Protein for the Modification of Gold Electrode Surfaces by Self-Assembled Monolayers

Design, Synthesis, and Use of Peptides Derived from Human Papillomavirus L1 Protein for the Modification of Gold Electrode Surfaces by Self-Assembled Monolayers

Electrochemical diagnostics of infectious viral diseases: Trends and challenges

Electrochemical Resistive DNA Biosensor for the Detection of HPV Type 16

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