Development of a capillary electrophoresis method for the screening of human urine for multiple drugs of abuse

Alnajjar, Ahmed O.; Idris, Abubakr M.; Multzenberg, Marika; McCord, Bruce

doi:10.1016/j.jchromb.2007.05.038

Cited by 26 publications

(17 citation statements)

References 29 publications

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“…Hyphenation with MS also makes possible accurate identification of the target compounds in this kind of complex sample. To the best of our knowledge, by applying in-line SPE-CE-MS we have obtained the lowest detection limits for these drugs in urine samples [2,6,8]. These LODs are very useful for rapid screening for illegal drugs in doping control or forensic analysis.…”

Section: Discussionmentioning

confidence: 89%

“…Different analytical methods based on liquid chromatography or gas chromatography, coupled to mass spectrometry for identification, have been developed for determination of drugs of abuse in environmental and biological samples [1][2][3][4]. Capillary electrophoresis (CE) has been proved to have great potential in the determination of drugs of abuse because of its excellent separation efficiency, low sample and solvent volumes, and high versatility in terms of separation modes [5][6][7]. These characteristics of CE make it suitable for the needs of forensic science laboratories [6,8,9].…”

Section: Introductionmentioning

confidence: 99%

“…Capillary electrophoresis (CE) has been proved to have great potential in the determination of drugs of abuse because of its excellent separation efficiency, low sample and solvent volumes, and high versatility in terms of separation modes [5][6][7]. These characteristics of CE make it suitable for the needs of forensic science laboratories [6,8,9].…”

Section: Introductionmentioning

confidence: 99%

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In-line solid-phase extraction–capillary electrophoresis coupled with mass spectrometry for determination of drugs of abuse in human urine

et al. 2012

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In-line solid-phase extraction-capillary electrophoresis coupled with mass spectrometric detection (SPE-CE-MS) has been used for determination of 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), codeine (COD), hydrocodeine (HCOD), and 6-acetylmorphine (6AM) in urine. The preconcentration system consists of a small capillary filled with Oasis HLB sorbent and inserted into the inlet section of the electrophoresis capillary. The SPE-CE-MS experimental conditions were optimized as follows: the sample (adjusted to pH 6.0) was loaded at 930 mbar for 60 min, elution was performed with methanol at 50 mbar for 35 s, 60 mmol L(-1) ammonium acetate at pH 3.8 was used as running buffer, the separation voltage was 30 kV, and the sheath liquid at a flow rate of 5.0 μL min(-1) was isopropanol-water 50:50 (v/v) containing 0.5% acetic acid. Analysis of urine samples spiked with the four drugs and diluted 1:1 (v/v) was studied in the linear range 0.08-10 ng mL(-1). Detection limits (LODs) (S/N = 3) were between 0.013 and 0.210 ng mL(-1). Repeatability (expressed as relative standard deviation) was below 7.2%. The method developed enables simple and effective determination of these drugs of abuse in urine samples at the levels encountered in toxicology and doping.

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Section: Discussionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In-line solid-phase extraction–capillary electrophoresis coupled with mass spectrometry for determination of drugs of abuse in human urine

et al. 2012

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“…LOD values varied between 5 and 30 ng/mL. As the method minimizes sample and solvent consumption, it was proposed for the analysis of drugs of abuse (Alnajjar et al, 2007). The enantiomers of salbutamol and bupivacaine were also separated by CE using dual mixtures of neutral CDs.…”

Section: Pharmaceuticalsmentioning

confidence: 99%

New applications of cyclodextrins in electrically driven chromatographic systems: a review

Cserháti

2008

Biomedical Chromatography

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“…There are also other methods described in the literature, such as oxidative colorimetric analysis [22], determination of opiates by circular dichroism [23], colorimetric determination [24], TLC analysis of opiate alkaloids and drugs [25,26], ELISA (enzyme-linked immunosorbent assay) analysis of thebaine [27], FTIR spectroscopy of opiates and chemometric analysis [28], differential pulse polarography [29] and even a voltammetric analysis of thebaine [30]. (6) and noscapine (7) T a b l e 2 [31], gas chromatography-ion trap mass spectrometry (GC-MS) analysis [32], liquid chromatographicatmospheric pressure chemical ionization mass spectrometric analysis [33] or HPLC analysis [34,35], GC-MS analysis [36][37][38][39]. Alkaloids are also being analyzed in environmental studies to trace the pollution of the environment [40].…”

Section: Introductionmentioning

confidence: 99%

Development of chromatographic methods for thebaine detection and quantification along with some of related alkaloid derivatives

Spasevska

Bogdanov

Babunovska³

2015

Maced. J. Chem. Chem. Eng.

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A RP-HPLC-UVD method for qualitative analysis and quantification of thebaine was developed utilizing RP-C18 monolithic column, gradient elution with trifluoroacetic acid and formic acid in water and in acetonitrile and detection wavelength of 285 nm. The effects of the relevant chromatographic conditions were investigated and the optimized and validated method was usеd for analysis of three in-house prepared batches of thebaine. Additionally, a complementary GC-MS method was developed for identification of thebaine and potential impurities. In parallel the batches were analyzed by routine methods for preliminary quality assessment based on physical and spectroscopic properties (melting point, specific optical rotation, UV-Vis and IR spectroscopy). There was correlation between the samples purity as determined by the developed HPLC method and the melting point ranges. The HPLC method herein presented is suitable for routine quantitative analysis regardless of the method of thebaine preparation and it is suitable for its corresponding free base or salt form. Additionally, considering the mobile phase compatibility, it can be adapted/transferred easily for HPLC-MS analysis, especially if the identity of the impurities needs to be determined.Keywords: thebaine; HPLC; purity; GC-MS; physical properties; spectroscopic properties; РАЗВОЈ НА ХРОМАТОГРАФСКИ МЕТОДИ ЗА ДЕТЕКЦИЈА И КВАНТИФИКАЦИЈА НА ТЕБАИН ЗАЕДНО СО НЕКОИ СРОДНИ АЛКАЛОИДНИ ДЕРИВАТИMетодатa RP-HPLC-UVD за квалитативна анализа и квантификација на тебаин е развиена со користење на монолитна колона RP-C18. Методата е со градиентна елуција со трифлуорооцетна и мравја киселина во вода и ацетонитрил и со детекција на 285 nm. Беа евалуирани ефектите на релевантните хроматографски услови и оптимизираната и валидирана метода беше применета за анализа на три подготвени серии тебаин. Дополнително беше развиена комплементарна GC-MS метода за идентификација на тебаин и потенцијалните нечистотии. Паралелно со хромато-графските методи, примероците на тебаин беа анализирани со рутински методи за прелиминарно одредување на квалитет, базирани на физички и спектроскопски својства (температура на топење, оптичка ротација, УВ-видлива и инфрацрвена спектроскопија). Постои корелација помеѓу чистотата на примероците одредена со HPLC и соодветните опсези на температурите на топење. Прикажаната HPLC метода е соодветна за рутинска анализа без разлика на методата на подготовка и може да се користи за тебаин како слободна база и за негови соли. Додатно, ако се земе предвид компатибилноста на мобилната фаза, развиената метода лесно може да се адаптира/трансферира за анализи со HPLC-MS, особено ако е потребно да се одреди идентитетот на нечистотиите.Клучни зборови: тебаин; HPLC; GC-MS; физички својства; спектроскопски својства; M. Spasevska, J. Bogdanov, H. Babunovska Maced. J. Chem. Chem. Eng. 34 (2), 231-243 (2015) 232

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Development of a capillary electrophoresis method for the screening of human urine for multiple drugs of abuse

Cited by 26 publications

References 29 publications

In-line solid-phase extraction–capillary electrophoresis coupled with mass spectrometry for determination of drugs of abuse in human urine

In-line solid-phase extraction–capillary electrophoresis coupled with mass spectrometry for determination of drugs of abuse in human urine

New applications of cyclodextrins in electrically driven chromatographic systems: a review

Development of chromatographic methods for thebaine detection and quantification along with some of related alkaloid derivatives

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