Development of a Cell Sheet Transportation Technique for Regenerative Medicine

Oie, Yoshinori; Nozaki, Taiji; Takayanagi, Hiroshi; Hara, Shotaro; Hayashi, Ryuhei; Takeda, Satomi; Mori, Keisuke; Moriya, Noboru; Soma, Takeshi; Tsujikawa, Motokazu; Saitō, Kazuo; Nishida, Kohji

doi:10.1089/ten.tec.2013.0266

Cited by 23 publications

(24 citation statements)

References 21 publications

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“…Overall, our results align with a study by Oie Y. et al . in 2014, where viability after transport control was shown to increase by ~4% following a 12 hour transport period at above-freezing temperature 33 . Our cell viability, cell death, PCNA, and Caspase-3 results indicated optimal maintenance of CES viability at 12 °C and 16 °C following one-week storage.…”

Section: Discussionmentioning

confidence: 99%

Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets

Jackson

Reppe

Eidet

et al. 2017

Sci Rep

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Cultured epidermal cell sheets (CES) containing undifferentiated cells are useful for treating skin burns and have potential for regenerative treatment of other types of epithelial injuries. The undifferentiated phenotype is therefore important for success in both applications. This study aimed to optimize a method for one-week storage of CES for their widespread distribution and use in regenerative medicine. The effect of storage temperatures 4 °C, 8 °C, 12 °C, 16 °C, and 24 °C on CES was evaluated. Analyses included assessment of viability, mitochondrial reactive oxygen species (ROS), membrane damage, mitochondrial DNA (mtDNA) integrity, morphology, phenotype and cytokine secretion into storage buffer. Lowest cell viability was seen at 4 °C. Compared to non-stored cells, ABCG2 expression increased between temperatures 8–16 °C. At 24 °C, reduced ABCG2 expression coincided with increased mitochondrial ROS, as well as increased differentiation, cell death and mtDNA damage. P63, C/EBPδ, CK10 and involucrin fluorescence combined with morphology observations supported retention of undifferentiated cell phenotype at 12 °C, transition to differentiation at 16 °C, and increased differentiation at 24 °C. Several cytokines relevant to healing were upregulated during storage. Importantly, cells stored at 12 °C showed similar viability and undifferentiated phenotype as the non-stored control suggesting that this temperature may be ideal for storage of CES.

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Section: Discussionmentioning

confidence: 99%

Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets

Jackson

Reppe

Eidet

et al. 2017

Sci Rep

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“…And since maintenance of the temperature and pressure is critical for cell viability during long distance transportation, several devices have been tested for use as safe and efficient transportation containers. Examples of such devices are those developed by Nozaki et al (2008) [100] and Oie et al (2014) [101] for transportation of the engineered tissues from a production facility to a medical operation facility while maintaining tissue viability and preventing contamination.…”

Section: Transportation Of Regenerated Tissuesmentioning

confidence: 99%

Tissue engineering in oral and maxillofacial reconstruction

Melek

2015

Tanta Dental Journal

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The artificial generation of tissues, organs, or even more complex living organisms was throughout the history of mankind a matter of myth and dream. During the last decades this vision became feasible and has been recently introduced in clinical medicine. The interest and attention that this rapidly developing area has received are based on the vision that the growing understanding of tissue healing and the achievements of biotechnology will be of profound therapeutic relevance. Clinically, reconstructive surgery has arrived at a standard of care that allows for repair and restoration of the vast majority of tissues/organs with established techniques. The real challenge of tissue engineering in clinical treatment is the reduction of surgical morbidity by the application of biological signals or bio-artificial components cultivated from the patient's own cells, that can replace the lost body part or accomplish its repair without the need for autogenous tissue transfer. Initially, activities in this area were mainly focused on cell-based approaches aiming at the generation of tissue-like constructs by combining ex vivo expanded cell populations with various types of scaffolds. Today, the field of tissue engineering has expanded tremendously, in that not only cells and scaffolds but also growth factors, controlled release carriers, engineering of biomaterials and many other areas of basic and applied research are considered to be part of the field of tissue engineering.

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“…34 We created a transportation container that performs the basic functions of maintaining interior temperature, air pressure, and sterility (Fig. 4).…”

Section: Multicenter Clinical Research Using Oral Mucosal Epithelial mentioning

confidence: 99%

Translational Research on Ocular Surface Reconstruction Using Oral Mucosal Epithelial Cell Sheets

Oie

Nishida

2014

Cornea

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Ocular surface reconstruction using autologous oral mucosal epithelial cell sheets has drastically changed the treatment of limbal stem-cell deficiency. The morphological and functional characteristics of oral mucosal epithelial cell sheets are similar to those of normal corneal epithelium. Ocular surface reconstruction can prevent potential problems associated with limbal transplantation, including immune rejection and donor tissue shortages. Thus far, ocular reconstruction using epithelial cell sheets has been limited to clinical research. Although the effectiveness and safety of this surgical approach have been confirmed to some extent, efforts to make its use more widespread are required. "Translational research" refers to the process of developing a new treatment based on basic research findings with useful practical applications in the field of health care. Medical centers for translational research are required to promote translational research in academic institutes. The Pharmaceutical Affairs Law was revised to promote technologies in the field of regenerative medicine in Japan. This article reviews translational research of ocular surface reconstruction using oral mucosal epithelial cell sheets.

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Development of a Cell Sheet Transportation Technique for Regenerative Medicine

Abstract: The newly developed transportation technique for air travel is essential technology for regenerative medicine and promotes the standardization and spread of regenerative therapies.

Cited by 23 publications

References 21 publications

Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets

Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets

Tissue engineering in oral and maxillofacial reconstruction

Translational Research on Ocular Surface Reconstruction Using Oral Mucosal Epithelial Cell Sheets

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