Development of a colloidal gold nanoparticle‐based immunochromatographic strip for the one‐step detection of miroestrol and puerarin

Abstract

Pueraria candollei or White Kwao Krua (Leguminosae) is an indigenous plant in Thailand which has long been used in Thai traditional medicine. The tuberous root of this plant is widely used for rejuvenation, particularly in elder women. Among the bioactive compounds in P. candollei, miroestrol and puerarin exhibit estrogenic activity. This study aims to develop an immunochromatographic strip (ICS) with a colloidal gold-based detection system for the simultaneous detection of miroestrol and puerarin in a one-step analysis. The developed method is sensitive and specific for the detection of miroestrol and puerarin in raw materials and marketed products. The detection limits of miroestrol and puerarin were 0.15 and 4.5 μg, respectively. In addition, the results from the developed ICS were confirmed with an enzyme-linked immunosorbent assay and presented a good correlation between these two methods. This is the first report on the development of an ICS that can detect miroestrol and puerarin in one step. The developed ICS provides a simplified method for the detection of miroestrol and puerarin in P. candollei and Pueraria spp.

Lateral flow immunoassay for small-molecules detection in phytoproducts: a review

“…The selection of these reactions depends on the structure of the target molecule. Considering that the reactive aldehyde is easily formed at the vicinal diol of the sugar part, which readily reacts with the amine group of the protein carrier, the sodium periodate reaction is typically preferred in the conjugation of sugar-containing compounds [37,39,42,51,[89][90][91][92][93][94]. However, the cross-reaction of the antibody against the aglycone of the glycosides should be considered when the antigen is prepared by this reaction.…”

“…Nevertheless, there are exceptions in certain haptens. For puerarin, pAb [ 94 ], and mAb [ 37 ] were applied in LFA and exhibited similar cross-reactivity profiles.…”

Analysis of Isoflavones in Pueraria by UHPLC-Q-Orbitrap HRMS and Study on α-Glucosidase Inhibitory Activity

“…Several methods have previously been developed for the analysis of pueraria components. These include enzyme-linked immunosorbent assay (ELISA) [ 19 , 20 ], high-performance liquid chromatography (HPLC) [ 21 , 22 ], and liquid chromatography-mass spectrometry (LC-MS/MS) [ 23 , 24 ]. However, most of these methods are limited to the determination of only 3 to 6 compounds.…”

Development of an Immunochromatographic Strip for Rapid Detection of H7 Subtype Avian Influenza Viruses

“…Compared with other detection methods, the immunochromatographic test strip labled with colloidal gold is more attractive because it is rapid and does not require extra equipment for detection [15]. Therefore, in this study McAbs were prepared using the inactivated H7N9 virus as an immunogen.…”