Development of a common PVS2 vitrification method for cryopreservation of several fruit and vegetable crops

Ромаданова, Н.В.; Кушнаренко, С. В.; Karasholakova, Lazzat

doi:10.1007/s11627-017-9849-y

Cited by 10 publications

(9 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[ 131 ], or ulluco ( Ullucus tuberosus Cal) [ 132 ], without maintaining a standard protocol for a large group, such as grains, fruits, and vegetables. However, a study published in 2017 was essential to adapt a basic protocol for the cryopreservation of a more diverse group of crops in the agronomic sector [ 133 ]. In this study, the shoot tips of four genera ( Malus , Solanum , Lonicera , and Berberis ) were tested with variations of cold acclimation, preculture media, and PVS2 exposure times.…”

Section: Cryopreservation Applications Of Agronomic Interestmentioning

confidence: 99%

Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

Roque‐Borda

Kulus

Souza

et al. 2021

IJMS

View full text Add to dashboard Cite

Numerous environmental and endogenous factors affect the level of genetic diversity in natural populations. Genetic variability is the cornerstone of evolution and adaptation of species. However, currently, more and more plant species and local varieties (landraces) are on the brink of extinction due to anthropopression and climate change. Their preservation is imperative for the sake of future breeding programs. Gene banks have been created worldwide to conserve different plant species of cultural and economic importance. Many of them apply cryopreservation, a conservation method in which ultra-low temperatures (−135 °C to −196 °C) are used for long-term storage of tissue samples, with little risk of variation occurrence. Cells can be successfully cryopreserved in liquid nitrogen (LN) when the adverse effect of ice crystal formation and growth is mitigated by the removal of water and the formation of the so-called biological glass (vitrification). This state can be achieved in several ways. The involvement of key cold-regulated genes and proteins in the acquisition of cold tolerance in plant tissues may additionally improve the survival of LN-stored explants. The present review explains the importance of cryostorage in agronomy and presents an overview of the recent works accomplished with this strategy. The most widely used cryopreservation techniques, classic and modern cryoprotective agents, and some protocols applied in crops are considered to understand which parameters provide the establishment of high quality and broadly applicable cryopreservation. Attention is also focused on the issues of genetic integrity and functional genomics in plant cryobiology.

show abstract

Section: Cryopreservation Applications Of Agronomic Interestmentioning

confidence: 99%

Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

Roque‐Borda

Kulus

Souza

et al. 2021

IJMS

View full text Add to dashboard Cite

show abstract

“…In the present study, the suitability of the cryopreservation method for the whole Finnish national blackcurrant collection was set as a goal. The droplet vitrification method was chosen to be optimized and used as a basic protocol for whole collection of blackcurrants, as it is reported to be a simple and universal protocol and easily implemented for wide range of accessions (Panis et al 2005;Romadanova et al 2017). Moreover, as the protocol was in use in our laboratory with some modifications (Nukari and Uosukainen 2007) for cryopreservation of several other species, the method was easy to implement for laboratory infrastructure and facilities available.…”

Section: Discussionmentioning

confidence: 99%

Droplet vitrification technique for cryopreservation of a large diversity of blackcurrant (Ribes nigrum L.) cultivars

Rantala

Kaseva

Nukari

et al. 2020

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

The aim of plant gene banks is to preserve genetic resources selected based on their phenotypic, agronomic, historical or other cultural values for future utilization. In the present study the modified PVS2 droplet vitrification technique was tested and optimized for cryopreservation of a large diversity of blackcurrant (R. nigrum L.) accessions propagated in vitro and selected into a national gene bank core collection. Out of four accessions tested to optimize the method, three recovered and regenerated by 89–97% on average, but one recalcitrant in vitro line only by 25%. The tested post-cryopreservation recovery media with different macronutrient and growth regulator levels showed no generalized effect on regenerated shoots, but the effect of recovery media was different between cultivars. When the whole regeneration chain from cryopreservation via micropropagation to greenhouse conditions was tested, shoots at least 1 cm in length were found necessary for successful transfer ex vitro. The long-term cryopreservation of 22 blackcurrant accessions was finally conducted, with practices slightly modified from the tested protocol. The estimated recovery of shoot tips after 9 weeks in vitro was 17–94% with at least 75% recovery in seven accessions and at least 40% recovery in 19 out of 22 accessions. Only one accession had no cryopreservation success. The results demonstrated that the modified droplet vitrification technique is appropriate for a large diversity of blackcurrant accessions. However, cultivar-related differences and recovery procedures are to be considered for success in regeneration and ex vitro adaptation.

show abstract

“…Наиболее часто применяют раствор PVS2 (Golmirzaie, Panta, 2000). Метод получил широкое распространение для разных культур, поскольку он проще в исполнении, чем более ранние методы криоконсервации (Romadanova et al, 2017). В ведущем генбанке картофеля CIP (Перу) с использованием метода витрификации с 1995 по 2000 год были криоконсервированы 197 генотипов картофеля со средним уровнем регенерации 46%.…”

Section: Cryoconservation Methods For Vegetatively Propagated Cropsunclassified

An Integrated Approach to Creating Scab-Resistant Apple: Phytopathological Testing and Marker-Assisted Selection

Супрун¹,

Насонов²,

Lоbodina³

et al. 2018

PBT&B

View full text Add to dashboard Cite

show abstract

Development of a common PVS2 vitrification method for cryopreservation of several fruit and vegetable crops

Cited by 10 publications

References 15 publications

Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies

Droplet vitrification technique for cryopreservation of a large diversity of blackcurrant (Ribes nigrum L.) cultivars

An Integrated Approach to Creating Scab-Resistant Apple: Phytopathological Testing and Marker-Assisted Selection

Contact Info

Product

Resources

About