Development of a competitive ELISA for detecting antibodies to the peste des petits ruminants virus using a recombinant nucleobrotein

Libeau, Geneviève; Préhaud, Christophe; Lancelot, Renaud; Colas, François; Guerre, L.; Bishop, David H. L.; Diallo, Adama

doi:10.1016/0034-5288(95)90088-8

Cited by 233 publications

(192 citation statements)

References 16 publications

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“…A confidence interval of 95%, a margin of error of 5%, an infinite population, an assumed true overall prevalence of 50% to obtain maximum sample size, and the sensitivity (94.5%) and specificity (99.4%) of the PPR competitive enzyme-linked immunosorbent assay (cELISA) [35] were used [36,37] in calculations of the sample size (PPR cELISA was used to calculate sample size as PPR was the main focus of the study). This gave the needed sample size of 435 samples for each of the two years when herds (containing sheep and/or goats) were visited.…”

Section: Methodsmentioning

confidence: 99%

“…Commercial enzyme-linked immunosorbent assay (ELISA) kits were used to analyse the presence of antibodies to the selected pathogens: ID screen PPR competition ELISA (detects anti-PPRV nucleoprotein antibodies [35], sensitivity 94.5%, specificity 99.4%; ID. Vet, Grabels, France), IDEXX CCPP Ab test (uses monoclonal antibody ‘4.52’ against Mycoplasma sp.…”

Section: Methodsmentioning

confidence: 99%

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Seroprevalence and risk factors for peste des petits ruminants and selected differential diagnosis in sheep and goats in Tanzania

Torsson

Berg

Misinzo

et al. 2017

Infection Ecology & Epidemiology

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Introduction: Livestock husbandry is critical for food security and poverty reduction in a low-income country like Tanzania. Infectious disease is one of the major constraints reducing the productivity in this sector. Peste des petits ruminants (PPR) is one of the most important diseases affecting small ruminants, but other infectious diseases may also be present. Objective: The objective of this study was to determine the seroprevalence and risk factors for exposure to PPR, contagious caprine pleuropneumonia (CCPP), foot-and-mouth disease (FMD), bluetongue (BT), and bovine viral diarrhoea (BVD) in sheep and goats in Tanzania. Methods: Serum samples were collected in 2014 and 2015, and analysed using enzyme-linked immunosorbent assays to detect antibodies to the five pathogens. Results and discussion: This is the first description of seroprevalence of FMD and BT among small ruminants in Tanzania. Risk factor analysis identified sex (female) (OR for 2014: PPR: 2.49, CCPP: 3.11, FMD: 2.98, BT: 12.4, OR for 2015: PPR: 14.1, CCPP: 1.10, FMD: 2.67, BT: 1.90, BVD: 4.73) and increasing age (>2 years) (OR for 2014: PPR: 14.9, CCPP: 2.34, FMD: 7.52, BT: 126, OR for 2015: PPR: 8.13, CCPP: 1.11, FMD: 2.98, BT: 7.83, BVD: 4.74) as risk factors for exposure to these diseases.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Seroprevalence and risk factors for peste des petits ruminants and selected differential diagnosis in sheep and goats in Tanzania

Torsson

Berg

Misinzo

et al. 2017

Infection Ecology & Epidemiology

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“…As a rapid, simple and sensitive assay, ELISA has been widely used in serological profiling of PPRV in mass screening of samples for sermonitoring/serosurveillance or clinical prevalence. Various workers have used MAb produced against PPRV and RPV for detection of antibodies and antigens in ELISA [2,83,117]. Saliki et al [118] and Anderson and Mckay [2], used neutralizing MAbs against H protein of PPRV for specific detection of PPRV antibodies in competitive ELISA (c-ELISA) and blocking ELISA (B-ELISA) [83,118,131,136] for antibody detection.…”

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

“…Various workers have used MAb produced against PPRV and RPV for detection of antibodies and antigens in ELISA [2,83,117]. Saliki et al [118] and Anderson and Mckay [2], used neutralizing MAbs against H protein of PPRV for specific detection of PPRV antibodies in competitive ELISA (c-ELISA) and blocking ELISA (B-ELISA) [83,118,131,136] for antibody detection. Use of the virus neutralising MAb was more advantageous as it produced better correlation between VNT and ELISA [83,117].…”

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

“…Saliki et al [118] and Anderson and Mckay [2], used neutralizing MAbs against H protein of PPRV for specific detection of PPRV antibodies in competitive ELISA (c-ELISA) and blocking ELISA (B-ELISA) [83,118,131,136] for antibody detection. Use of the virus neutralising MAb was more advantageous as it produced better correlation between VNT and ELISA [83,117]. The sensitivity and specificity of B-ELISA was found to be 90.4 and 98.8 %, respectively when compared to VNT [117].…”

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

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Diagnosis and control of peste des petits ruminants: a comprehensive review

Balamurugan¹,

Hemadri²,

Gajendragad³

et al. 2013

VirusDis.

135

118

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Peste des petits ruminants (PPR) is an acute, highly contagious, world organization for animal health (OIE) notifiable and economically important transboundary viral disease of sheep and goats associated with high morbidity and mortality and caused by PPR virus. PPR is considered as one of the main constraints in augmenting the productivity of small ruminants in developing countries and particularly severely affects poor farmer's economy. The disease is clinically manifested by pyrexia, oculo-nasal discharges, necrotizing and erosive stomatitis, gastroenteritis, diarrhoea and bronchopneumonia. The disease can be diagnosed from its clinical signs, pathological lesions, and specific detection of virus antigen/antibodies/genome in the clinical samples by various serological tests and molecular assays. PPR is the one of the priority animal diseases whose control is considered important for poverty alleviation in enzootic countries. Availability of effective and safe live attenuated cell culture PPR vaccines and diagnostics have boosted the recently launched centrally sponsored control programme in India and also in other countries. This review article primarily focus on the current scenario of PPR diagnosis and its control programme with advancement of research areas that have taken place in the recent years with future perspectives.

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Peste des Petits Ruminants

Diallo¹,

Singh²

2021

Veterinary Vaccines

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Development of a competitive ELISA for detecting antibodies to the peste des petits ruminants virus using a recombinant nucleobrotein

Cited by 233 publications

References 16 publications

Seroprevalence and risk factors for peste des petits ruminants and selected differential diagnosis in sheep and goats in Tanzania

Seroprevalence and risk factors for peste des petits ruminants and selected differential diagnosis in sheep and goats in Tanzania

Diagnosis and control of peste des petits ruminants: a comprehensive review

Peste des Petits Ruminants

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