Development of a “Continuous-Flow Adhesion Cell” for the Assessment of Hydrogel Adhesion

Abstract

The purpose of this study was to develop an in vitro perfusion technique or "continuous-flow adhesion cell" model to predict the in vivo performances of different mucoadhesive drug delivery systems based on hydrogels. Two studies were performed, either using a rabbit small intestine or a polyethylene surface; the adhesion of four gels--two poly(acrylic acid)s (PAAs) (carbomer [CM] and polycarbophil [PC]), an ethyleneoxide-propyleneoxide block copolymer (Poloxamer 407 [PM]), and a polysaccharide (scleroglucane [SG])--were evaluated. In this respect, scleroglucane was used as a control. The adhesiveness of the different gels for both supports is in accordance with that described in the literature, that is, polycarbophil adhered more strongly than carbomer, which itself adhered more strongly than poloxamer. This study proved that the gels adhere more strongly to the polyethylene tube than to the rabbit small intestine, thus indicating that evidence for adhesion properties does not need any presence of mucus. Therefore, our in vitro model could be a good method, more precise and more simple than an ex vivo technique, to predict the bioadhesion of gelified devices.

A Review of Poloxamer 407 Pharmaceutical and Pharmacological Characteristics

“…Retaining the drug in the rectum is a very important factor in avoiding first-pass hepatic elimination. Le Ray et al (53) have performed (bio)adhesion tests using galls tube made of flexible inert material (polyethylene) or freshly excised rabbit small intestine membrane. A dynamic continuous-flow adhesion cell was developed by the authors.…”

Pluronic F-68 and F-127 Based Nanomedicines for Advancing Combination Cancer Therapy

“…Therefore, retaining the drug maximally in the rectum is an important consideration to prevent first-pass hepatic elimination. The visualization of the adhesive properties of Pluronic F-127 gel was performed by incorporating a colored marker (such as Ponceau S) into Pluronic F-127 gel [ 73 ]. Suppositories with a colorant (such as Blue n°1 lake 0.1% w / w ) were administered in the rectums of rats to verify bioadhesive behavior and migration distance.…”