Development of a designed comparison method based on isotope dilution liquid chromatography–tandem mass spectrometry for determining plasma renin activity and its clinical assessment of renin activity stability in plasma

Liu, Zhenni; Jin, Lizi; Zhang, Jiangtao; Zhang, Tianjiao; Zeng, Jie; Zhou, Weiyan; Zhang, Chuanbao

doi:10.1039/d2ay01646j

Cited by 3 publications

(2 citation statements)

References 27 publications

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“…[1][2][3] Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) is one of the most powerful analytical techniques for quantitative bioanalysis. [4][5][6] Study of LC-MS/MS analysis for quantication of nucleobases, nucleosides, and nucleotides has been widely reported, 7,8 and reviews on this subject can be found in the literature. 9,10 In an LC-MS/MS analysis of biological uids such as urine, a sample pretreatment by solid-phase extraction (SPE) is normally carried out to isolate the analytes from the complex sample matrix.…”

Section: Introductionmentioning

confidence: 99%

“…It involves the conversion of these compounds to their respective ethenoderivatives with chloroacetaldehyde (CAA). CAA is known to react with nucleobases, forming etheno-derivatives: 1,N 6 etheno-adenine (3-A), 3,N 4 -etheno-cytosine (3-C), N 2,3 -etheno-guanine (N2,3-3-G), and 1,N 2 -etheno-guanine (1,N 2 -3-G). 1,27 It's expected that the increased structural aromaticity in ethenoderivatives overcomes the difficulties associated with the high hydrophilicity of intact nucleobases and nucleosides in the LC-MS analysis.…”

Section: Introductionmentioning

confidence: 99%

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An on-line SPE-LC-MS/MS method for quantification of nucleobases and nucleosides present in biological fluids

Liu,

Wang,

Dickenson

et al. 2024

Anal. Methods

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

An on-line SPE-LC-MS/MS method for quantification of nucleobases and nucleosides present in biological fluids

Liu,

Wang,

Dickenson

et al. 2024

Anal. Methods

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Laboratory Testing for Endocrine Hypertension: Current and Future Perspectives

Courcelles,

Stoenoiu,

Haufroid

et al. 2024

Clinical Chemistry

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Background Secondary hypertension (SH) is a form of high blood pressure caused by an identifiable underlying condition. Although, it accounts for a small fraction of the overall hypertensive population, detection and management of SH is of utmost importance, because SH phenotypes carry a high cardiovascular risk and can possibly be cured by timely treatment. Content This review focuses on the endocrine causes of SH, such as primary aldosteronism, Cushing syndrome, thyroid disease, pheochromocytoma and paraganglioma, acromegaly, and rare monogenic forms. It discusses current biomarkers, analytical methods, and diagnostic strategies, highlighting advantages and limitations of each approach. It also explores the emerging -omics technologies that can provide a comprehensive and multidimensional assessment of SH and its underlying mechanisms. Summary Endocrine SH is a heterogeneous and complex condition that requires proper screening and confirmatory tests to avoid diagnostic delays and improve patient outcomes. Careful biomarker interpretation is essential due to potential interferences, variability, and method-dependent differences. Liquid chromatography–tandem mass spectrometry is a superior method for measuring low-concentration hormones and metabolites involved in SH, but it requires expertise. Omics approaches have great potential to identify novel biomarkers, pathways, and targets for SH diagnosis and treatment, especially considering its multifactorial nature.

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Commutability assessment of candidate reference materials for plasma renin activity measurement: current challenges

Liu,

Jin,

et al. 2023

Clinical Chemistry and Laboratory Medicine (CCLM)

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Objectives This study aims to evaluate the commutability of external quality assessment (EQA) materials and candidate reference materials (RMs) for plasma renin activity (PRA) assay. Methods Commutabilities of 16 candidate RMs were measured along with 40 clinical samples by the four different routine PRA assays, including three LC‒MS/MS assays and one chemiluminescence immunoassay. Sixteen candidate RMs included native/spiked human plasma pools (small-scale pools with <50 individuals) and current EQA materials (large-scale pools with >1,000 individuals). Difference in bias approach and linear regression with prediction interval approach were adopted to determine the commutability. Two-way variance analysis was used to estimate the effects of spiked and pool size on the commutability. Stability and homogeneity studies were performed. Results Precision and correlation performance of all assays was acceptable. In the difference in bias approach, the commutability results were not satisfactory (noncommutability: 14/16) and significant sample-specific effects were detected in assay pairs using different incubation buffers. For the prediction interval approach, no commutability was observed in the spiked small-scale pools; EQA materials (4/9) had more satisfactory commutability among all assays than the small-scale pools (2/7); RMs of large-scale pools tend to have better commutability no matter spiked or not. Conclusions Commutable RMs were obtainable but challenging. Current EQA materials with relatively good commutability, stability, and homogeneity were appropriate RMs. Large-scale pools are tending to be commutable. Spiking in small-scale pools was not suggested to prepare RMs. MPs adopting a uniform incubation buffer would be preferable for further commutability research.

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Development of a designed comparison method based on isotope dilution liquid chromatography–tandem mass spectrometry for determining plasma renin activity and its clinical assessment of renin activity stability in plasma

Abstract: This study explored the optimized plasma incubation strategies and a traceable, robust, and reproducible designed comparison method based on LC-MS/MS for quantifying plasma renin activity, which is expected to harmonize laboratory measurement.

Cited by 3 publications

References 27 publications

An on-line SPE-LC-MS/MS method for quantification of nucleobases and nucleosides present in biological fluids

An on-line SPE-LC-MS/MS method for quantification of nucleobases and nucleosides present in biological fluids

Laboratory Testing for Endocrine Hypertension: Current and Future Perspectives

Commutability assessment of candidate reference materials for plasma renin activity measurement: current challenges

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