Development of a dispersive liquid–liquid microextraction method for the determination of fluoroquinolones in chicken liver by high performance liquid chromatography

Moema, D.; Nindi, Mathew Muzi; Dube, Simiso

doi:10.1016/j.aca.2011.11.036

Cited by 82 publications

(59 citation statements)

References 36 publications

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“…The incorporation of UAE and SPE techniques in the 35 sample preparation allows for exhaustive extraction of the target analytes and effective clean-up of the sample matrices prior to instrumental analysis. The described method has been validated with good specificity, excellent sensitivity, and satisfactory accuracy.…”

Section: Discussionmentioning

confidence: 99%

“…Figure S2 demonstrates the influence of the liquid-to-solid ratio on the extraction yield of six selected FQs (NOR, CIP, PEF, DAN, ENR, and MAR). The results indicated that an increase of extraction efficiency of FQs could be observed 35 with the increase of the solvent volume from 5 to 10 mL, and maintained almost constant (above 92%) from 10 to 30 mL. Hence, 10 mL was selected as the volume of extraction solvent.…”

Section: Optimization Of Sample Pretreatment Conditionsmentioning

confidence: 95%

“…In view of the fact that chemical nature of column packing 35 materials may have a significant effect on chromatographic performance, a wide variety of commercially available UPLC columns with sub-2 μm particles were compared for the separation of the 16 FQs during method development, such as BEH C 18 shape and detection sensitivity. The packing particles of the BEH Shield RP18 column are synthesized on high-pressure bearing ethylene bridged hybrid (BEH) substrate, and contain an embedded carbamate polar group.…”

Section: Optimization Of Uplc-ms/ms Conditionsmentioning

confidence: 99%

“…Ultraperformance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-MS/MS) was applied for the separation and analysis of the multicomponent FQ analytes. 35 …”

Section: Introductionmentioning

confidence: 97%

“…In general, the vast majority of cosmetics manufacturers act responsibly, and voluntary addition of prohibited ingredients is very scarce. 35 However, in order to achieve anti-acne, anti-freckle, anti-wrinkle, skin-whitening or astringent efficacy in a short term, illicit substances, e.g., antibiotics 2 and corticosteroids 3,4 , might be intentionally added to cosmetic products by a few lawbreakers for economic benefit, hence posing a serious risk to human health. 40 Fluoroquinolones (FQs) are a large group of broad-spectrum synthetic antibiotics, which have been initially used for the treatment of the infectious diseases of humans and foodproducing animals, since they are effective against both Grampositive and Gram-negative bacteria 5 .…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Simultaneous determination of 16 fluoroquinolone antibiotics in cosmetics by ultra-performance liquid chromatography/triple quadrupole mass spectrometry with ultrasound-assisted extraction and solid-phase extraction

Meng

Zhang

et al. 2015

Anal. Methods

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Section: Discussionmentioning

confidence: 99%

Section: Optimization Of Sample Pretreatment Conditionsmentioning

confidence: 95%

Section: Optimization Of Uplc-ms/ms Conditionsmentioning

confidence: 99%

“…Ultraperformance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-MS/MS) was applied for the separation and analysis of the multicomponent FQ analytes. 35 …”

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Simultaneous determination of 16 fluoroquinolone antibiotics in cosmetics by ultra-performance liquid chromatography/triple quadrupole mass spectrometry with ultrasound-assisted extraction and solid-phase extraction

Meng

Zhang

et al. 2015

Anal. Methods

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Determination of levofloxacin, ciprofloxacin, moxifloxacin and gemifloxacin in urine and plasma by HPLC–FLD–DAD using pentafluorophenyl core–shell column: Application to drug monitoring

Yıldırım

Karakoç

Yaşar

et al. 2020

Biomedical Chromatography

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Concentrations of fluoroquinolones, which are used in the treatment of many bacterial infections, should be monitored in biological fluids as they exhibit concentration‐dependent bactericidal activity. In this study, a liquid chromatography method for the determination of levofloxacin, ciprofloxacin, moxifloxacin and gemifloxacin in human urine and plasma was developed for the first time. The efficiency of five different columns for the separation of these fluoroquinolones was compared. Experimental parameters that affect the separation, such as percentage of organic solvent, pH, temperature, gradient shape and detector wavelength, were optimized by a step‐by‐step approach. Using a pentafluorophenyl core–shell column (100 × 4.6 mm, 2.7 μm), the separation of four analytes was accomplished in <7.5 min. The developed method was validated for the determination of analytes in both urine and plasma with respect to sensitivity, specificity, linearity (r ≥ 0.9989), recovery (79.46–102.69%), accuracy, precision and stability (85.79–111.07%). The intra‐ and inter‐day accuracies were within 89.55–111.94% with relative standard deviations of 0.35–8.05%. The feasibility of method was demonstrated by analyzing urine and plasma samples of patients orally receiving levofloxacin, ciprofloxacin or moxifloxacin. The developed method is suitable for therapeutic drug monitoring of these fluoroquinolones and can be applied to pharmacokinetic and toxicological studies.

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Liquid‐liquid Extraction Coupled to Batch Injection Analysis for Electroanalysis of Levofloxacin at Low Concentration Level

et al. 2017

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This work presents the integration between phase‐separation by magnetic‐stirring salt‐induced high‐temperature liquid‐liquid extraction (PS‐MSSI‐HT‐LLE) and batch injection analysis with amperometric detection (BIA‐AD) as an alternative strategy for pre‐concentration of analytes before hydrodynamic electroanalysis. To demonstrate the performance of this analytical system, the emerging contaminant levofloxacin was quantified in tap, aquarium and lake water at low concentration level. In the optimized conditions, BIA‐AD enabled fast (160 h−1) and reproducible results (RSD<2 %) and the PS‐MSSI‐HT‐LLE allowed the detection of levofloxacin concentration levels not detected by direct electroanalysis (70 and 80 nmol L−1) corresponding to 100‐folds enrichment factors. The performance of proposed method was evaluated by addition‐recovery test and was obtained satisfactory recovery values (between 70 and 96 %). Moreover, PS‐MSSI‐HT‐LLE allows the pre‐concentration of many samples simultaneously, which is advantageous over pre‐concentration on working electrode surface (stripping methods).

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Development of a dispersive liquid–liquid microextraction method for the determination of fluoroquinolones in chicken liver by high performance liquid chromatography

Cited by 82 publications

References 36 publications

Simultaneous determination of 16 fluoroquinolone antibiotics in cosmetics by ultra-performance liquid chromatography/triple quadrupole mass spectrometry with ultrasound-assisted extraction and solid-phase extraction

Simultaneous determination of 16 fluoroquinolone antibiotics in cosmetics by ultra-performance liquid chromatography/triple quadrupole mass spectrometry with ultrasound-assisted extraction and solid-phase extraction

Determination of levofloxacin, ciprofloxacin, moxifloxacin and gemifloxacin in urine and plasma by HPLC–FLD–DAD using pentafluorophenyl core–shell column: Application to drug monitoring

Liquid‐liquid Extraction Coupled to Batch Injection Analysis for Electroanalysis of Levofloxacin at Low Concentration Level

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