Development of a DIVA (Differentiating Infected from Vaccinated Animals) strategy using a vaccine containing a heterologous neuraminidase for the control of avian influenza

Capua, Ilaria; Terregino, Calogero; Cattoli, Giovanni; Mutinelli, Franco; Rodrı́guez, José F.

doi:10.1080/0307945021000070714

Cited by 179 publications

(107 citation statements)

References 8 publications

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“…Use of vaccination without properly designed serological surveillance will prevent detection of infections in vaccinated flocks and hamper implementation of science-based changes to control programmes, thus greatly limiting eradication efforts (Capua et al ., 2003;Swayne, 2003). Traditional AGP, ELISA and HI serological tests detect antibodies against nucleoprotein/matrix protein and haemagglutinin, respectively, induced by vaccination with inactivated whole AI vaccines.…”

Section: Discussionmentioning

confidence: 99%

“…Traditional AGP, ELISA and HI serological tests detect antibodies against nucleoprotein/matrix protein and haemagglutinin, respectively, induced by vaccination with inactivated whole AI vaccines. To detect infections in such vaccinated populations, special provisions must be preplanned and implemented, such as the use of AGP and HI tests in unvaccinated sentinels or the use of special tests to detect antibodies to non-structural protein or heterologous neuraminidase in vaccinated birds (Beard, 1987;Swayne & Suarez, 2000;Capua et al ., 2003;Swayne, 2003;Tumpey et al ., 2005). In the current study, AGP, H5 HI, and N2 NI antibodies were demonstrated in H5N2 AI vaccinated birds.…”

Section: Discussionmentioning

confidence: 99%

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Inactivated North American and European H5N2 avian influenza virus vaccines protect chickens from Asian H5N1 high pathogenicity avian influenza virus

2006

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High-pathogenicity (HP) avian influenza (AI) virus of the H5N1 subtype has caused an unprecedented epizootic in birds within nine Asian countries/regions since it was first reported in 1996. Vaccination has emerged as a tool for use in managing the infection in view of future eradication. This study was undertaken to determine whether two divergent H5N2 commercial vaccine strains, one based on a European and the other a North American low-pathogenicity AI virus, could protect chickens against a recent Asian H5N1 HPAI virus. The North American and European vaccine viruses had 84 and 91% deduced amino acid sequence similarity to the HA1 segment of haemagglutinin protein of Indonesia H5N1 HPAI challenge virus, respectively. Both vaccine strains provided complete protection from clinical signs and death. The vaccines reduced the number of chickens infected and shedding virus from the respiratory and intestinal tracts at the peak of virus replication. In addition, the quantity of virus shed was reduced by 10 4 to 10 5 median embryo infectious doses. The use of specific neuraminidase inhibition tests allowed identification of infected chickens within the vaccinated groups. These data indicate that the currently available H5 vaccines of European and North American lineages will protect chickens against the Asian H5N1 HPAI virus and reduce environmental contamination by the H5N1 HPAI virus. They will be an adjunct to biosecurity measures to reduce virus transmission.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Inactivated North American and European H5N2 avian influenza virus vaccines protect chickens from Asian H5N1 high pathogenicity avian influenza virus

2006

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“…3,4 Briefly, uncertainty regarding the true value of the Se and Sp of the test and the disease prevalence (p) was modeled using probability distributions referred to as prior distributions. Prior values for the cELISA, based on the uncertainty for the Se and Sp values, were modeled using data reported in peer-reviewed literature [9][10][11]15,27 and at professional conferences (Rigoni M, Viale E, Mancin M, et al: 2009, Development of a competitive ELISA system for the detection of influenza A virus nucleoprotein antibodies and validation in five species of poultry. In: Proceedings of the World Association of Veterinary Laboratory Diagnosticians 14th International Symposium, p. 174.…”

Section: Methodsmentioning

confidence: 99%

A Diagnostic Algorithm for Detection of Antibodies to Influenza A Viruses in Dogs in Italy (2006–2008)

Benedictis

Anderson²,

Pérez

et al. 2010

J VET Diagn Invest

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Abstract. Since 2004, there have been several reports of Influenza A virus (FLUAV) infection in dogs. Dogs have been infected with equine influenza H3N8, avian influenza H3N2 and H5N1, and the pandemic H1N1 virus. Because of recent avian and equine influenza outbreaks in Italy, the objectives of the present study were to estimate the level of exposure of Italian dogs to influenza A viruses and to assess a diagnostic algorithm for detection of FLUAV exposure in dogs. Sera collected from 6,858 dogs from 2006 to 2008 were screened in a competitive enzyme-linked immunosorbent assay (cELISA) for antibodies to the highly conserved influenza A nucleoprotein. Samples positive in the cELISA were confirmed by testing in hemagglutination inhibition (HI) and fluorescent antibody test (FAT). Two seropositive dogs had antibodies to H3 hemagglutinin proteins, consistent with exposure to recent canine and equine subtype H3N8 viruses. Using a Bayesian model, the sensitivity and specificity of the cELISA were estimated as 93.98% (probability intervals [PI]: 81.67-99.08%) and 98.71% (PI: 98.43-98.96%), respectively. After accounting for the imperfect sensitivity and specificity of the cELISA, the Bayesian posterior prevalence of FLUAV exposure among tested Italian dogs was 0.5% (PI: 0.1-1.4%). The study results indicate that screening with a cELISA for influenza A nucleoprotein antibody, followed by confirmatory testing with HI and/or FAT, is a highly sensitive and highly specific approach for diagnosing FLUAV exposure in dogs.

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“…The sera of vaccinated birds were tested by an indirect immunofluorescence (iIFAT) anti-N1 antibody detection test (Capua et al, 2003).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the protection induced by avian influenza vaccines containing a 1994 Mexican H5N2 LPAI seed strain against a 2008 Egyptian H5N1 HPAI virus belonging to clade 2.2.1 by means of serological andin vivotests

et al. 2010

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Since 2006 Egypt has been facing an extensive epidemic of H5N1 highly pathogenic avian influenza (HPAI) with a huge number of outbreaks both in rural and intensively reared poultry areas. The use of efficacious vaccines in this country has been, and still remains, essential for the control and possible eradication of HPAI. The present study was performed to establish whether the administration of inactivated vaccines containing an H5 virus belonging to a different lineage to the Eurasian H5N1 HPAI viruses guarantees protection from clinical signs, provides significant immune response and is able to achieve a reduction of viral shedding in the face of a challenge with a contemporary H5N1 virus isolated in Egypt. Despite the genetic and antigenic differences between the vaccine strain (H5N2/Mexico) and the challenge strain (H5N1/ Egypt), confirmed by molecular and serological (haemagglutination inhibition) tests, it was established that the immune response induced by these conventional vaccines is sufficient to prevent infection in the majority of birds challenged with a contemporary H5N1 Egyptian strain. The data reported in this study also indicate that there may be a low degree of correlation between haemagglutination inhibition titres, clinical protection and reduction of shedding.

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Development of a DIVA (Differentiating Infected from Vaccinated Animals) strategy using a vaccine containing a heterologous neuraminidase for the control of avian influenza

Cited by 179 publications

References 8 publications

Inactivated North American and European H5N2 avian influenza virus vaccines protect chickens from Asian H5N1 high pathogenicity avian influenza virus

Inactivated North American and European H5N2 avian influenza virus vaccines protect chickens from Asian H5N1 high pathogenicity avian influenza virus

A Diagnostic Algorithm for Detection of Antibodies to Influenza A Viruses in Dogs in Italy (2006–2008)

Evaluation of the protection induced by avian influenza vaccines containing a 1994 Mexican H5N2 LPAI seed strain against a 2008 Egyptian H5N1 HPAI virus belonging to clade 2.2.1 by means of serological andin vivotests

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